ID   E5_VACCW                Reviewed;         341 AA.
AC   P21606; Q76ZW0;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   23-FEB-2022, entry version 68.
DE   RecName: Full=Protein E5;
GN   OrderedLocusNames=VACWR061; ORFNames=E5R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2398897; DOI=10.1128/mcb.10.10.5433-5441.1990;
RA   Ahn B.-Y., Gershon P.D., Jones E.V., Moss B.;
RT   "Identification of rpo30, a vaccinia virus RNA polymerase gene with
RT   structural similarity to a eucaryotic transcription elongation factor.";
RL   Mol. Cell. Biol. 10:5433-5441(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   INDUCTION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=10854161; DOI=10.1016/s0168-1702(98)00114-2;
RA   Murcia-Nicolas A., Bolbach G., Blais J.C., Beaud G.;
RT   "Identification by mass spectroscopy of three major early proteins
RT   associated with virosomes in vaccinia virus-infected cells.";
RL   Virus Res. 59:1-12(1999).
CC   -!- FUNCTION: Major early protein present in virus factories.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm. Note=Localizes in cytoplasmic
CC       virus factories.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:10854161}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae E5 family. {ECO:0000305}.
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DR   EMBL; M36339; AAB59825.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89340.1; -; Genomic_DNA.
DR   PIR; D35928; D35928.
DR   RefSeq; YP_232943.1; NC_006998.1.
DR   SMR; P21606; -.
DR   DNASU; 3707594; -.
DR   GeneID; 3707594; -.
DR   KEGG; vg:3707594; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   InterPro; IPR018379; BEN_domain.
DR   InterPro; IPR004334; Poxvirus_E5R.
DR   Pfam; PF10523; BEN; 2.
DR   PIRSF; PIRSF015691; VAC_E5R; 1.
DR   PROSITE; PS51457; BEN; 2.
PE   1: Evidence at protein level;
KW   Early protein; Host cytoplasm; Reference proteome; Repeat.
FT   CHAIN           1..341
FT                   /note="Protein E5"
FT                   /id="PRO_0000099452"
FT   DOMAIN          112..222
FT                   /note="BEN 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00784"
FT   DOMAIN          233..328
FT                   /note="BEN 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00784"
SQ   SEQUENCE   341 AA;  40380 MW;  B74B44B2CFB5054C CRC64;
     MLILTKVNIY MLIIVLWLYG YNFIISESQC PMINDDSFTL KRKYQIDSAE STIKMDKKRT
     KFQNRAKMVK EINQTIRAAQ THYETLKLGY IKFKRMIRTT TLEDIAPSIP NNQKTYKLFS
     DISAIGKASR NPSKMVYALL LYMFPNLFGD DHRFIRYRMH PMSKIKHKIF SPFKLNLIRI
     LVEERFYNNE CRSNKWRIIG TQVDKMLIAE SDKYTIDARY NLKPMYRIKG KSEEDTLFIK
     QMVEQCVTSQ ELVEKVLKIL FRDLFKSGEY KAYRYDDDVE NGFIGLDTLK LNIVHDIVEP
     CMPVRRPVAK ILCKEMVNKY FENPLHIIGK NLQECIDFVS E