ID   E6_VACCW                Reviewed;         567 AA.
AC   P21607; Q76ZV9;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   29-SEP-2021, entry version 62.
DE   RecName: Full=Protein E6;
GN   OrderedLocusNames=VACWR062; ORFNames=E6R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2398897; DOI=10.1128/mcb.10.10.5433-5441.1990;
RA   Ahn B.-Y., Gershon P.D., Jones E.V., Moss B.;
RT   "Identification of rpo30, a vaccinia virus RNA polymerase gene with
RT   structural similarity to a eucaryotic transcription elongation factor.";
RL   Mol. Cell. Biol. 10:5433-5441(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION.
RX   PubMed=19217136; DOI=10.1016/j.virol.2009.01.009;
RA   Resch W., Weisberg A.S., Moss B.;
RT   "Expression of the highly conserved vaccinia virus E6 protein is required
RT   for virion morphogenesis.";
RL   Virology 386:478-485(2009).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=20116821; DOI=10.1016/j.virol.2010.01.012;
RA   Boyd O., Turner P.C., Moyer R.W., Condit R.C., Moussatche N.;
RT   "The E6 protein from vaccinia virus is required for the formation of
RT   immature virions.";
RL   Virology 399:201-211(2010).
RN   [5]
RP   FUNCTION, AND INDUCTION.
RC   STRAIN=Mutant Cts52;
RX   PubMed=20116822; DOI=10.1016/j.virol.2010.01.010;
RA   Boyd O., Strahl A.L., Rodeffer C., Condit R.C., Moussatche N.;
RT   "Temperature-sensitive mutant in the vaccinia virus E6 protein produce
RT   virions that are transcriptionally inactive.";
RL   Virology 399:221-230(2010).
RN   [6]
RP   FUNCTION.
RX   PubMed=25863879; DOI=10.1016/j.virol.2015.02.056;
RA   Condit R.C., Moussatche N.;
RT   "The vaccinia virus E6 protein influences virion protein localization
RT   during virus assembly.";
RL   Virology 482:147-156(2015).
CC   -!- FUNCTION: Late protein which plays an essential role in virion
CC       assembly. Participates in the correct localization and aggregation of
CC       the viral core proteins. {ECO:0000269|PubMed:19217136,
CC       ECO:0000269|PubMed:20116821, ECO:0000269|PubMed:20116822,
CC       ECO:0000269|PubMed:25863879}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:20116821}.
CC       Note=Localizes in the mature virion (MV).
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:20116822}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae E6 family. {ECO:0000305}.
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DR   EMBL; M36339; AAB59826.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89341.1; -; Genomic_DNA.
DR   PIR; E35928; E35928.
DR   RefSeq; YP_232944.1; NC_006998.1.
DR   DNASU; 3707595; -.
DR   GeneID; 3707595; -.
DR   KEGG; vg:3707595; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0019068; P:virion assembly; IDA:UniProtKB.
DR   InterPro; IPR006749; Pox_E6.
DR   Pfam; PF04656; Pox_E6; 1.
DR   PIRSF; PIRSF015629; VAC_E6R; 1.
PE   2: Evidence at transcript level;
KW   Late protein; Reference proteome; Virion.
FT   CHAIN           1..567
FT                   /note="Protein E6"
FT                   /id="PRO_0000099455"
FT   VARIANT         226
FT                   /note="P -> L (in strain: Mutant Cts52)"
SQ   SEQUENCE   567 AA;  66727 MW;  01BD7C94E149B61E CRC64;
     MDFIRRKYLI YTVENNIDFL KDDTLSKVNN FTLNHVLALK YLVSNFPQHV ITKDVLANTN
     FFVFIHMVRC CKVYEAVLRH AFDAPTLYVK ALTKNYLSFS NTIQSYKETV HKLTQDEKFL
     EVAKYMDELG ELIGVNYDLV LNPLFHGGEP IKDMEIIFLK LFKKTDFKVV KKLSVIRLLI
     WAYLSKKDTG IEFADNDRQD IYTLFQQTGR IVHSNLTETF RDYIFPGDKT SYWVWLNESI
     ANDADIVLNR HAITMYDKIL SYIYSEIKQG RVNKNMLKLV YIFEPEKDIR ELLLEIIYDI
     PGDILSIIDA KNDDWKKYFI SFYKANFING NTFISDRTFN EDLFRVVVQI DPEYFDNERI
     MSLFSTSAAD IKRFDELDIN NSYISNIIYE VNDITLDTMD DMKKCQIFNE DTSYYVKEYN
     TYLFLHESDP MVIENGILKK LSSIKSKSRR LNLFSKNILK YYLDGQLARL GLVLDDYKGD
     LLVKMINHLK SVEDVSAFVR FSTDKNPSIL PSLIKTILAS YNISIIVLFQ RFLRDNLYHV
     EEFLDKSIHL TKTDKKYILQ LIRHGRS