E70B1_ARATH
ID E70B1_ARATH Reviewed; 624 AA.
AC Q9FGH9;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Exocyst complex component EXO70B1 {ECO:0000303|PubMed:23944713};
DE Short=AtExo70b1 {ECO:0000303|PubMed:23944713};
DE AltName: Full=Exocyst subunit Exo70 family protein B1 {ECO:0000303|PubMed:23944713};
GN Name=EXO70B1 {ECO:0000303|PubMed:23944713};
GN OrderedLocusNames=At5g58430 {ECO:0000312|Araport:AT5G58430};
GN ORFNames=MQJ2.3 {ECO:0000312|EMBL:BAB10258.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, INTERACTION WITH EXO70B2; SEC5A AND EXO84B, SUBCELLULAR LOCATION,
RP AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=23944713; DOI=10.1111/tra.12101;
RA Kulich I., Pecenkova T., Sekeres J., Smetana O., Fendrych M., Foissner I.,
RA Hoeftberger M., Zarsky V.;
RT "Arabidopsis exocyst subcomplex containing subunit EXO70B1 is involved in
RT the autophagy-related transport to the vacuole.";
RL Traffic 14:1155-1165(2013).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH PUB18, REGULATION BY
RP PUB18, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=27956469; DOI=10.1105/tpc.16.00347;
RA Seo D.H., Ahn M.Y., Park K.Y., Kim E.Y., Kim W.T.;
RT "The N-terminal UND motif of the Arabidopsis U-box E3 ligase PUB18 is
RT critical for the negative regulation of ABA-mediated stomatal movement and
RT determines its ubiquitination specificity for exocyst subunit Exo70B1.";
RL Plant Cell 28:2952-2973(2016).
CC -!- FUNCTION: Component of an exocyst subcomplex specifically involved in
CC autophagy-related, Golgi-independent membrane traffic to the vacuole.
CC Regulates autophagosome formation and autophagy-related Golgi-
CC independent import into the vacuole. Positive regulator of both
CC abscisic acid (ABA)-promoted and mannitol (drought)-promoted stomatal
CC closure (PubMed:27956469). {ECO:0000269|PubMed:23944713,
CC ECO:0000269|PubMed:27956469}.
CC -!- SUBUNIT: Interacts with EXO70B2, SEC5A AND EXO84B (PubMed:23944713).
CC Binds to PUB18 (PubMed:27956469). {ECO:0000269|PubMed:23944713,
CC ECO:0000269|PubMed:27956469}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, phagosome
CC {ECO:0000269|PubMed:23944713}. Endomembrane system
CC {ECO:0000269|PubMed:27956469}. Note=Displays a punctate pattern.
CC Colocalizes with PUB18. {ECO:0000269|PubMed:27956469}.
CC -!- PTM: Target of the E3 ubiquitin-protein ligase PUB18 that mediates its
CC ubiquitination and degradation via the 26S proteasome.
CC {ECO:0000269|PubMed:27956469}.
CC -!- DISRUPTION PHENOTYPE: Twisted leaves with small spontaneous lesions,
CC early yellowing of leaves and anthocyanin accumulation defects.
CC Chlorotic shoots. Retarded growth with wrinkled rosette leaves under
CC long-day growth conditions. Impaired abscisic acid (ABA)-mediated and
CC mannitol (drought)-promoted stomatal closure (PubMed:27956469).
CC {ECO:0000269|PubMed:23944713, ECO:0000269|PubMed:27956469}.
CC -!- SIMILARITY: Belongs to the EXO70 family. {ECO:0000305}.
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DR EMBL; AB025632; BAB10258.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97052.1; -; Genomic_DNA.
DR EMBL; AY094480; AAM19847.1; -; mRNA.
DR EMBL; BT000827; AAN33202.1; -; mRNA.
DR RefSeq; NP_200651.1; NM_125229.4.
DR AlphaFoldDB; Q9FGH9; -.
DR SMR; Q9FGH9; -.
DR BioGRID; 21200; 4.
DR IntAct; Q9FGH9; 1.
DR STRING; 3702.AT5G58430.1; -.
DR iPTMnet; Q9FGH9; -.
DR PaxDb; Q9FGH9; -.
DR PRIDE; Q9FGH9; -.
DR ProteomicsDB; 222036; -.
DR EnsemblPlants; AT5G58430.1; AT5G58430.1; AT5G58430.
DR GeneID; 835956; -.
DR Gramene; AT5G58430.1; AT5G58430.1; AT5G58430.
DR KEGG; ath:AT5G58430; -.
DR Araport; AT5G58430; -.
DR TAIR; locus:2171208; AT5G58430.
DR eggNOG; KOG2344; Eukaryota.
DR HOGENOM; CLU_010236_2_0_1; -.
DR InParanoid; Q9FGH9; -.
DR OMA; DHPIWAD; -.
DR OrthoDB; 410847at2759; -.
DR PhylomeDB; Q9FGH9; -.
DR PRO; PR:Q9FGH9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FGH9; baseline and differential.
DR Genevisible; Q9FGH9; AT.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
DR GO; GO:0000145; C:exocyst; IPI:TAIR.
DR GO; GO:0070062; C:extracellular exosome; IDA:TAIR.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0045335; C:phagocytic vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IDA:TAIR.
DR GO; GO:0052542; P:defense response by callose deposition; IMP:TAIR.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0006955; P:immune response; IMP:TAIR.
DR GO; GO:0009789; P:positive regulation of abscisic acid-activated signaling pathway; IMP:UniProtKB.
DR GO; GO:0090333; P:regulation of stomatal closure; IMP:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IMP:UniProtKB.
DR InterPro; IPR016159; Cullin_repeat-like_dom_sf.
DR InterPro; IPR004140; Exo70.
DR InterPro; IPR046364; Exo70_C.
DR PANTHER; PTHR12542; PTHR12542; 1.
DR Pfam; PF03081; Exo70; 1.
DR SUPFAM; SSF74788; SSF74788; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Membrane; Reference proteome; Transport;
KW Ubl conjugation.
FT CHAIN 1..624
FT /note="Exocyst complex component EXO70B1"
FT /id="PRO_0000424566"
FT REGION 148..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 624 AA; 70640 MW; F2D87F19B72D838F CRC64;
MAENGEEKLL AVARHIAKTL GHNESMADDI LQIFSNFDGR FSREKLAEGQ AGEDGSGVAT
LERALNSIDG QISRFVAADQ PIWADPADSA AFLDTIDELV AIIREWSPMA SEKPIGICLT
RADDMMQQAM FRIEEEFRSL MERGAESFGL NPQGDAGAMN HRFDSEEEED DDRDFNNGDD
IQIPVAQPLT DYDLIIDALP SATINDLHEM AKRMLGAGFG KACSHVYSSC RREFLEESMS
RLGLQKLSIE EVHKMPWQEL EDEIDRWIKA ANVALRILFP SERRLCDRVF FGFSSAADLS
FMEVCRGSTI QLLNFADAIA IGSRSPERLF KVLDVFETMR DLMPEFESVF SDQFCSVLRN
EAVTIWKRLG EAIRGIFMEL ENLIRRDPAK AAVPGGGLHP ITRYVMNYLR AACRSRQTLE
QVFEESNGVP SKDSTLLTVQ MSWIMELLES NLEVKSKVYK DPALCYVFLM NNGRYIVQKV
KDGDLGLLLG DDWIRKHNVK VKQYHMNYQR SSWNKMLGLL KVDNTAAGMN GLGKTMKEKL
KQFNIQFDEI CKVHSTWVVF DEQLKEELKI SLARLLVPAY GSFIGRFQNL GDIGKNADKY
IKYGVEDIEA RINELFKGTT TGRK
