E74EA_DROME
ID E74EA_DROME Reviewed; 829 AA.
AC P20105; Q9VVI8;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=Ecdysone-induced protein 74EF isoform A;
DE AltName: Full=ETS-related protein E74A;
GN Name=Eip74EF; Synonyms=E74; ORFNames=CG32180;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), AND ALTERNATIVE SPLICING.
RC STRAIN=Canton-S;
RX PubMed=2107982; DOI=10.1016/0092-8674(90)90217-3;
RA Burtis K.C., Thummel C.S., Jones C.W., Karim F.D., Hogness D.S.;
RT "The Drosophila 74EF early puff contains E74, a complex ecdysone-inducible
RT gene that encodes two ets-related proteins.";
RL Cell 61:85-99(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=8223281; DOI=10.1242/dev.118.2.613;
RA Huet F., Ruiz C., Richards G.;
RT "Puffs and PCR: the in vivo dynamics of early gene expression during
RT ecdysone responses in Drosophila.";
RL Development 118:613-627(1993).
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A; Synonyms=E74A;
CC IsoId=P20105-1; Sequence=Displayed;
CC Name=B; Synonyms=D, E74B;
CC IsoId=P11536-1; Sequence=External;
CC -!- DEVELOPMENTAL STAGE: In mid instar larvae salivary glands levels are
CC low during puff stage 1 and increase during puff stage 2 to become the
CC predominant form in stage 3. Levels reach maximum in late larvae during
CC puff stages 8-10, decreasing abruptly at stage 11. This expression
CC pattern is also seen in Malpighian tubules and wing disk. Levels at
CC puff stage 11 are appreciable in the gut and fat body. Transcripts are
CC detected again in salivary glands from puff stages 12-14 and 17-21.
CC {ECO:0000269|PubMed:8223281}.
CC -!- INDUCTION: The expression of this protein is developmentally regulated
CC and is correlated with the 20-OH-ecdysone induced activity of puff
CC 74EF.
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
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DR EMBL; M37082; AAA28493.1; -; mRNA.
DR EMBL; AE014296; AAF49323.2; -; Genomic_DNA.
DR PIR; A34692; A34692.
DR RefSeq; NP_730287.1; NM_168740.3. [P20105-1]
DR AlphaFoldDB; P20105; -.
DR SMR; P20105; -.
DR BioGRID; 65250; 2.
DR IntAct; P20105; 2.
DR EnsemblMetazoa; FBtr0075202; FBpp0074965; FBgn0000567. [P20105-1]
DR GeneID; 39962; -.
DR CTD; 39962; -.
DR FlyBase; FBgn0000567; Eip74EF.
DR VEuPathDB; VectorBase:FBgn0000567; -.
DR GeneTree; ENSGT00940000169052; -.
DR SignaLink; P20105; -.
DR BioGRID-ORCS; 39962; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Eip74EF; fly.
DR GenomeRNAi; 39962; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0000567; Expressed in embryonic/larval hemocyte (Drosophila) and 22 other tissues.
DR ExpressionAtlas; P20105; baseline and differential.
DR Genevisible; P20105; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006914; P:autophagy; IMP:FlyBase.
DR GO; GO:0008219; P:cell death; TAS:FlyBase.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0048477; P:oogenesis; NAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0040034; P:regulation of development, heterochronic; TAS:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0035071; P:salivary gland cell autophagic cell death; IMP:FlyBase.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849; PTHR11849; 1.
DR Pfam; PF00178; Ets; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..829
FT /note="Ecdysone-induced protein 74EF isoform A"
FT /id="PRO_0000204082"
FT DNA_BIND 733..815
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT REGION 214..237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 301..329
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 353..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 396..443
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 508..533
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 558..641
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 214..228
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 405..439
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 509..533
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 558..618
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 829 AA; 87139 MW; 7C8B07CF0A1D7F5D CRC64;
MPFIDDALLW CPDNDGRLVG GLDLGTCIAD DSTANGTENL NPSIQSAGNP NNPQQSVGGE
ILGSVESAGN ELNGAAARNV NVVVEPLCGG DSSDELFRSF SESNFEIESL LSDLATVEVK
VENEENNNNV ITDDDFASVA AAVVANDDLL AKENAQLSAQ GLVDSVAASL ADSGDAGGQQ
ALLAFGSSSS AASAIAAAAA ALCGDLINNN NNNSNSNNNS NGNGNHGGGG GGASSGGGVA
GDCATKLEYA LMGGQPLAEE PRFVTSAAAN PLLVEKLMSK CLNIEKRMDK LSDTEIPIVK
QSTSPAPQQQ LQQQHHLQQQ QQQQPHNGST FAGATALLHI KTEQNTLLTP LQLQQQQQQQ
QGLHGAAGNG GSSNGNNAHQ QQQPLAIPQR PLLHNLLSGG AIHNPHHRNY TTATTGSFPP
SPADSGVSDV DSSSSGGQPC ADELKARLGM PPATSASAAA AAAAAAAAAA HLHTGTFLHP
NLYQNNAANS LRNIWNRSVG VPDNYYGSSG AGSGGTQPGG PGNPQTPGYL TTSYFNAPTA
ATAAASQRGT TINGYHSLHQ QQQQQQQSQQ SQQQQQLAHQ QLSHQQQQAL HQQLSHQQQQ
QQQQQQQHPH SQLNGPHPHS HPHSHPHSHP HAGQHTHSTI AAAAAAAAAS VVSSSSSAVA
AAAMLSASAA AAATAAAAAG GSQSVIQPAT SSVSYDLSYM LELGGFQQRK AKKPRKPKLE
MGVKRRSREG STTYLWEFLL KLLQDREYCP RFIKWTNREK GVFKLVDSKA VSRLWGMHKN
KPDMNYETMG RALRYYYQRG ILAKVDGQRL VYQFVDVPKD IIEIDCNGV
