E74EB_DROME
ID E74EB_DROME Reviewed; 883 AA.
AC P11536; A4V233; Q86NS8; Q9VVI7;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 188.
DE RecName: Full=Ecdysone-induced protein 74EF isoform B;
DE AltName: Full=ETS-related protein E74B;
GN Name=Eip74EF; Synonyms=E74; ORFNames=CG32180;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2501755; DOI=10.1093/nar/17.12.4455;
RA Janknecht R., Taube W., Luedecke H.-J., Pongs O.;
RT "Characterization of a putative transcription factor gene expressed in the
RT 20-OH-ecdysone inducible puff 74EF in Drosophila melanogaster.";
RL Nucleic Acids Res. 17:4455-4464(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), AND ALTERNATIVE SPLICING.
RC STRAIN=Canton-S;
RX PubMed=2107982; DOI=10.1016/0092-8674(90)90217-3;
RA Burtis K.C., Thummel C.S., Jones C.W., Karim F.D., Hogness D.S.;
RT "The Drosophila 74EF early puff contains E74, a complex ecdysone-inducible
RT gene that encodes two ets-related proteins.";
RL Cell 61:85-99(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=8223281; DOI=10.1242/dev.118.2.613;
RA Huet F., Ruiz C., Richards G.;
RT "Puffs and PCR: the in vivo dynamics of early gene expression during
RT ecdysone responses in Drosophila.";
RL Development 118:613-627(1993).
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B; Synonyms=D, E74B;
CC IsoId=P11536-1; Sequence=Displayed;
CC Name=A; Synonyms=E74A;
CC IsoId=P20105-1; Sequence=External;
CC -!- DEVELOPMENTAL STAGE: In mid instar larvae salivary glands levels
CC increase during 86-94 hours of development and represent the
CC predominant isoform during puff stage 1. Levels remain relatively
CC constant in late larvae until the premetamorphic pulse of ecdysone.
CC Transcripts are detected again from puff stages 12-14 and 17-21.
CC {ECO:0000269|PubMed:8223281}.
CC -!- INDUCTION: The expression of this protein is developmentally regulated
CC and is correlated with the 20-OH-ecdysone induced activity of puff
CC 74EF.
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
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DR EMBL; X15087; CAA33195.1; -; Genomic_DNA.
DR EMBL; M37083; AAA28494.1; -; mRNA.
DR EMBL; AE014296; AAF49324.1; -; Genomic_DNA.
DR EMBL; AE014296; AAX52740.1; -; Genomic_DNA.
DR EMBL; BT003745; AAO41406.1; -; mRNA.
DR PIR; S04722; S04722.
DR RefSeq; NP_001014590.1; NM_001014590.2. [P11536-1]
DR RefSeq; NP_730288.1; NM_168741.2. [P11536-1]
DR AlphaFoldDB; P11536; -.
DR SMR; P11536; -.
DR BioGRID; 65250; 2.
DR IntAct; P11536; 2.
DR STRING; 7227.FBpp0099825; -.
DR PaxDb; P11536; -.
DR PRIDE; P11536; -.
DR EnsemblMetazoa; FBtr0075204; FBpp0074967; FBgn0000567. [P11536-1]
DR EnsemblMetazoa; FBtr0100411; FBpp0099825; FBgn0000567. [P11536-1]
DR GeneID; 39962; -.
DR KEGG; dme:Dmel_CG32180; -.
DR CTD; 39962; -.
DR FlyBase; FBgn0000567; Eip74EF.
DR VEuPathDB; VectorBase:FBgn0000567; -.
DR eggNOG; KOG3804; Eukaryota.
DR GeneTree; ENSGT00940000169052; -.
DR HOGENOM; CLU_014368_0_0_1; -.
DR InParanoid; P11536; -.
DR OMA; APDCREF; -.
DR PhylomeDB; P11536; -.
DR Reactome; R-DME-8939245; RUNX1 regulates transcription of genes involved in BCR signaling.
DR Reactome; R-DME-8939247; RUNX1 regulates transcription of genes involved in interleukin signaling.
DR SignaLink; P11536; -.
DR BioGRID-ORCS; 39962; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Eip74EF; fly.
DR GenomeRNAi; 39962; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0000567; Expressed in embryonic/larval hemocyte (Drosophila) and 22 other tissues.
DR ExpressionAtlas; P11536; baseline and differential.
DR Genevisible; P11536; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006914; P:autophagy; IMP:FlyBase.
DR GO; GO:0008219; P:cell death; TAS:FlyBase.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0048477; P:oogenesis; NAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0040034; P:regulation of development, heterochronic; TAS:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0035071; P:salivary gland cell autophagic cell death; IMP:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849; PTHR11849; 1.
DR Pfam; PF00178; Ets; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..883
FT /note="Ecdysone-induced protein 74EF isoform B"
FT /id="PRO_0000204083"
FT DNA_BIND 787..869
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT REGION 19..207
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 223..264
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 299..321
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 355..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 407..437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 450..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 562..587
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 612..695
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..61
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..77
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..119
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..157
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 191..207
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 223..238
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 243..264
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 459..493
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 563..587
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 612..672
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 400
FT /note="T -> A (in Ref. 5; AAO41406)"
FT /evidence="ECO:0000305"
FT CONFLICT 867
FT /note="Q -> H (in Ref. 1; CAA33195)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 883 AA; 94811 MW; 063AA031A18D1409 CRC64;
MIMVQHLVAA SAHNFASQAA ASLVNVSSSS SSSSSSSSSS LSLSSSSSSS SLSSATPTPV
ASPVTPTSPP PAAAAPAEAS PPAGAELQED GQQAKTQEDP TMKDQDMLEK TRQEVKDPVN
VEEPGAIVDT ESVMARQSPS PVASTKVPES LEEISNKSPP VQEDEEESES VASDCREFKV
LYNHLRQQQH HHSPSSPDKT RSTLDDVSKI LWERKQQLQR SSVITAAPTL QPQQHQQPMS
DIEDEETLED VDDADADVEA DAEDEELLEQ YQNGYDSPLD LSLGGATSAA ASAAAAASAV
SRRRGRTYSG TESDDSAQCE RARMRLKPER KAERSAAYKK SLMKRYYTEI PIVKQSTSPA
PQQQLQQQHH LQQQQQQQPH NGSTFAGATA LLHIKTEQNT LLTPLQLQQQ QQQQQGLHGA
AGNGGSSNGN NAHQQQQPLA IPQRPLLHNL LSGGAIHNPH HRNYTTATTG SFPPSPADSG
VSDVDSSSSG GQPCADELKA RLGMPPATSA SAAAAAAAAA AAAAHLHTGT FLHPNLYQNN
AANSLRNIWN RSVGVPDNYY GSSGAGSGGT QPGGPGNPQT PGYLTTSYFN APTAATAAAS
QRGTTINGYH SLHQQQQQQQ QSQQSQQQQQ LAHQQLSHQQ QQALHQQLSH QQQQQQQQQQ
QHPHSQLNGP HPHSHPHSHP HSHPHAGQHT HSTIAAAAAA AAASVVSSSS SAVAAAAMLS
ASAAAAATAA AAAGGSQSVI QPATSSVSYD LSYMLELGGF QQRKAKKPRK PKLEMGVKRR
SREGSTTYLW EFLLKLLQDR EYCPRFIKWT NREKGVFKLV DSKAVSRLWG MHKNKPDMNY
ETMGRALRYY YQRGILAKVD GQRLVYQFVD VPKDIIEIDC NGV
