E75BB_DROME
ID E75BB_DROME Reviewed; 1412 AA.
AC P13055; Q9VVM9;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 2.
DT 03-AUG-2022, entry version 204.
DE RecName: Full=Ecdysone-induced protein 75B, isoform B;
DE AltName: Full=E75-C;
DE AltName: Full=Nuclear receptor subfamily 1 group D member 3, isoform B;
GN Name=Eip75B; Synonyms=NR1D3; ORFNames=CG8127;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Canton-S; TISSUE=Head;
RX PubMed=2508058; DOI=10.1093/nar/17.18.7167;
RA Feigl G., Gram M., Pongs O.;
RT "A member of the steroid hormone receptor gene family is expressed in the
RT 20-OH-ecdysone inducible puff 75B in Drosophila melanogaster.";
RL Nucleic Acids Res. 17:7167-7178(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=8223281; DOI=10.1242/dev.118.2.613;
RA Huet F., Ruiz C., Richards G.;
RT "Puffs and PCR: the in vivo dynamics of early gene expression during
RT ecdysone responses in Drosophila.";
RL Development 118:613-627(1993).
CC -!- FUNCTION: Implicated in the regulation of ecdysone-triggered gene
CC hierarchies. Probably plays a key role in mediating the regulation of
CC the larval molt by 20-OH-ecdysone. {ECO:0000269|PubMed:8223281}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=B; Synonyms=E75C;
CC IsoId=P13055-2; Sequence=Displayed;
CC Name=C; Synonyms=E75A;
CC IsoId=P17671-1; Sequence=External;
CC Name=A; Synonyms=E75B;
CC IsoId=P17672-1; Sequence=External;
CC Name=D;
CC IsoId=P17671-2; Sequence=External;
CC -!- DEVELOPMENTAL STAGE: In mid instar larvae salivary glands, levels
CC increase during puff stage 1, then remain relatively constant until the
CC premetamorphic pulse of ecdysone at puff stage 5. Levels increase again
CC in late larvae at puff stages 9-10. At puff stage 1 expression is also
CC seen in the gut. Levels are low in the gut, Malpighian tubules, fat
CC body and wing disks between stages 1 and 11.
CC {ECO:0000269|PubMed:8223281}.
CC -!- INDUCTION: The expression of this protein is developmentally regulated
CC and is correlated with the 20-OH-ecdysone induced activity of puff 75B.
CC {ECO:0000269|PubMed:8223281}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR1
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA33611.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X15586; CAA33611.1; ALT_FRAME; mRNA.
DR EMBL; AE014296; AAF49282.3; -; Genomic_DNA.
DR PIR; S05979; S05979.
DR RefSeq; NP_001246821.1; NM_001259892.1. [P13055-2]
DR RefSeq; NP_001246822.1; NM_001259893.2. [P13055-2]
DR RefSeq; NP_730321.1; NM_168755.1. [P13055-2]
DR AlphaFoldDB; P13055; -.
DR SMR; P13055; -.
DR BioGRID; 65284; 18.
DR IntAct; P13055; 9.
DR STRING; 7227.FBpp0297726; -.
DR PaxDb; P13055; -.
DR PRIDE; P13055; -.
DR DNASU; 39999; -.
DR EnsemblMetazoa; FBtr0075148; FBpp0074914; FBgn0000568. [P13055-2]
DR EnsemblMetazoa; FBtr0306813; FBpp0297725; FBgn0000568. [P13055-2]
DR EnsemblMetazoa; FBtr0306814; FBpp0297726; FBgn0000568. [P13055-2]
DR GeneID; 39999; -.
DR KEGG; dme:Dmel_CG8127; -.
DR CTD; 39999; -.
DR FlyBase; FBgn0000568; Eip75B.
DR VEuPathDB; VectorBase:FBgn0000568; -.
DR eggNOG; KOG3575; Eukaryota.
DR InParanoid; P13055; -.
DR OMA; IVRHHQQ; -.
DR PhylomeDB; P13055; -.
DR Reactome; R-DME-200425; Carnitine metabolism.
DR Reactome; R-DME-204174; Regulation of pyruvate dehydrogenase (PDH) complex.
DR Reactome; R-DME-381340; Transcriptional regulation of white adipocyte differentiation.
DR Reactome; R-DME-383280; Nuclear Receptor transcription pathway.
DR Reactome; R-DME-400206; Regulation of lipid metabolism by PPARalpha.
DR Reactome; R-DME-4090294; SUMOylation of intracellular receptors.
DR Reactome; R-DME-5362517; Signaling by Retinoic Acid.
DR Reactome; R-DME-9616222; Transcriptional regulation of granulopoiesis.
DR Reactome; R-DME-9707564; Cytoprotection by HMOX1.
DR SignaLink; P13055; -.
DR BioGRID-ORCS; 39999; 1 hit in 3 CRISPR screens.
DR ChiTaRS; Eip75B; fly.
DR GenomeRNAi; 39999; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0000568; Expressed in brain and 27 other tissues.
DR ExpressionAtlas; P13055; baseline and differential.
DR Genevisible; P13055; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0020037; F:heme binding; IDA:FlyBase.
DR GO; GO:0004879; F:nuclear receptor activity; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0018990; P:ecdysis, chitin-based cuticle; IMP:FlyBase.
DR GO; GO:0009755; P:hormone-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0007591; P:molting cycle, chitin-based cuticle; IMP:FlyBase.
DR GO; GO:0061060; P:negative regulation of peptidoglycan recognition protein signaling pathway; IMP:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:FlyBase.
DR GO; GO:0048477; P:oogenesis; NAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007553; P:regulation of ecdysteroid metabolic process; IMP:FlyBase.
DR GO; GO:0010468; P:regulation of gene expression; IDA:FlyBase.
DR GO; GO:0035075; P:response to ecdysone; IMP:FlyBase.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001728; ThyrH_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR PRINTS; PR00546; THYROIDHORMR.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Metal-binding;
KW Nucleus; Receptor; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..1412
FT /note="Ecdysone-induced protein 75B, isoform B"
FT /id="PRO_0000053505"
FT DOMAIN 565..813
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 455..531
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 458..478
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 495..514
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 1..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 110..204
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 258..298
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 321..448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 837..878
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 984..1021
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1044..1064
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1108..1174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1204..1317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1368..1401
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..55
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..96
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 264..286
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 321..401
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 416..448
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 839..878
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 989..1020
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1044..1062
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1110..1132
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1147..1174
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1368..1389
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 20
FT /note="V -> F (in Ref. 1; CAA33611)"
FT /evidence="ECO:0000305"
FT CONFLICT 183
FT /note="Q -> QQ (in Ref. 1; CAA33611)"
FT /evidence="ECO:0000305"
FT CONFLICT 337..342
FT /note="Missing (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 1142
FT /note="S -> V (in Ref. 1; CAA33611)"
FT /evidence="ECO:0000305"
FT CONFLICT 1216
FT /note="V -> L (in Ref. 1; CAA33611)"
FT /evidence="ECO:0000305"
FT CONFLICT 1231
FT /note="Missing (in Ref. 1; CAA33611)"
FT /evidence="ECO:0000305"
FT CONFLICT 1302
FT /note="N -> I (in Ref. 1; CAA33611)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1412 AA; 151292 MW; 6F4C21B075443F8C CRC64;
MEAVQAAAAA TSSGGSSGSV PGSGSGSASK LIKTEPIDFE MLHLEENERQ QDIEREPSSS
NSNSNSNSLT PQRYTHVQVQ TVPPRQPTGL TTPGGTQKVI LTPRVEYVQQ RATSSTGGGM
KHVYSQQQGT AASRSAPPET TALLTTTSGT PQIIITRTLP SNQHLSRRHS ASPSALHHYQ
QQQPQRQQSP PPLHHQQQQQ QQHVRVIRDG RLYDEATVVV AARRHSVSPP PLHHHSRSAP
VSPVIARRGG AAAYMDQQYQ QRQTPPLAPP PPPPPPPPPP PPPQQQQQQY ISTGVPPPTA
AARKFVVSTS TRHVNVIASN HFQQQQQQHQ AQQHQQQHQQ HQQHQQHVIA SVSSSSSSSA
IGSGGSSSSH IFRTPVVSSS SSSNMHHQQQ QQQQQSSLGN SVMRPPPPPP PPKVKHASSS
SSGNSSSSNT NNSSSSSNGE EPSSSIPDLE FDGTTVLCRV CGDKASGFHY GVHSCEGCKG
FFRRSIQQKI QYRPCTKNQQ CSILRINRNR CQYCRLKKCI AVGMSRDAVR FGRVPKREKA
RILAAMQQST QNRGQQRALA TELDDQPRLL AAVLRAHLET CEFTKEKVSA MRQRARDCPS
YSMPTLLACP LNPAPELQSE QEFSQRFAHV IRGVIDFAGM IPGFQLLTQD DKFTLLKAGL
FDALFVRLIC MFDSSINSII CLNGQVMRRD AIQNGANARF LVDSTFNFAE RMNSMNLTDA
EIGLFCAIVL ITPDRPGLRN LELIEKMYSR LKGCLQYIVA QNRPDQPEFL AKLLETMPDL
RTLSTLHTEK LVVFRTEHKE LLRQQMWSME DGNNSDGQQN KSPSGSWADA MDVEAAKSPL
GSVSSTESAD LDYGSPSSSQ PQGVSLPSPP QQQPSALASS APLLAATLSG GCPLRNRANS
GSSGDSGAAE MDIVGSHAHL TQNGLTITPI VRHQQQQQQQ QQIGILNNAH SRNLNGGHAM
CQQQQQHPQL HHHLTAGAAR YRKLDSPTDS GIESGNEKNE CKAVSSGGSS SCSSPRSSVD
DALDCSDAAA NHNQVVQHPQ LSVVSVSPVR SPQPSTSSHL KRQIVEDMPV LKRVLQAPPL
YDTNSLMDEA YKPHKKFRAL RHREFETAEA DASSSTSGSN SLSAGSPRQS PVPNSVATPP
PSAASAAAGN PAQSQLHMHL TRSSPKASMA SSHSVLAKSL MAEPRMTPEQ MKRSDIIQNY
LKRENSTAAS STTNGVGNRS PSSSSTPPPS AVQNQQRWGS SSVITTTCQQ RQQSVSPHSN
GSSSSSSSSS SSSSSSSSTS SNCSSSSASS CQYFQSPHST SNGTSAPASS SSGSNSATPL
LELQVDIADS AQPLNLSKKS PTPPPSKLHA LVAAANAVQR YPTLSADVTV TASNGGPPSA
AASPAPSSSP PASVGSPNPG LSAAVHKVML EA
