E75BC_DROME
ID E75BC_DROME Reviewed; 1199 AA.
AC P17671; B3DN68; B3DN69; Q8IQS1; Q8IQS3;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 2.
DT 03-AUG-2022, entry version 197.
DE RecName: Full=Ecdysone-induced protein 75B, isoforms C/D;
DE AltName: Full=E75-A;
DE AltName: Full=Nuclear receptor subfamily 1 group D member 3, isoforms C/D;
GN Name=Eip75B; Synonyms=NR1D3; ORFNames=CG8127;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), AND ALTERNATIVE SPLICING.
RC STRAIN=Canton-S;
RX PubMed=2110921; DOI=10.1101/gad.4.2.204;
RA Segraves W.A., Hogness D.S.;
RT "The E75 ecdysone-inducible gene responsible for the 75B early puff in
RT Drosophila encodes two new members of the steroid receptor superfamily.";
RL Genes Dev. 4:204-219(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS C AND D).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=8223281; DOI=10.1242/dev.118.2.613;
RA Huet F., Ruiz C., Richards G.;
RT "Puffs and PCR: the in vivo dynamics of early gene expression during
RT ecdysone responses in Drosophila.";
RL Development 118:613-627(1993).
CC -!- FUNCTION: Implicated in the regulation of ecdysone-triggered gene
CC hierarchies. Probably plays a key role in mediating the regulation of
CC the larval molt by 20-OH-ecdysone. {ECO:0000269|PubMed:8223281}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=C; Synonyms=E75A;
CC IsoId=P17671-1; Sequence=Displayed;
CC Name=A; Synonyms=E75B;
CC IsoId=P17672-1; Sequence=External;
CC Name=B; Synonyms=E75C;
CC IsoId=P13055-2; Sequence=External;
CC Name=D;
CC IsoId=P17671-2; Sequence=VSP_014915, VSP_014916;
CC -!- DEVELOPMENTAL STAGE: In mid instar larvae salivary glands, low basal
CC levels are observed in puff stage 1. Levels increase in late larvae
CC from puff stages 3-10, then decrease abruptly at stage 11. In prepupae,
CC isoform C is the predominant form during the transition between puff
CC stages 18-19. At puff stage 1, expression is also present in the gut.
CC By stage 3 it is present in the wing disks, Malpighian tubules and the
CC fat body. At stage 11, expression is only present in the gut and wing
CC disks. {ECO:0000269|PubMed:8223281}.
CC -!- INDUCTION: The expression of this protein is developmentally regulated
CC and is correlated with the 20-OH-ecdysone induced activity of puff 75B.
CC {ECO:0000269|PubMed:8223281}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR1
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA35923.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X51548; CAA35923.1; ALT_FRAME; mRNA.
DR EMBL; AE014296; AAN11687.1; -; Genomic_DNA.
DR EMBL; AE014296; AAN11689.1; -; Genomic_DNA.
DR EMBL; BT032856; ACD81870.1; -; mRNA.
DR EMBL; BT032857; ACD81871.1; -; mRNA.
DR PIR; A34598; A34598.
DR RefSeq; NP_730322.1; NM_168756.2. [P17671-1]
DR RefSeq; NP_730323.1; NM_168757.1. [P17671-2]
DR AlphaFoldDB; P17671; -.
DR SMR; P17671; -.
DR BioGRID; 65284; 18.
DR IntAct; P17671; 3.
DR DNASU; 39999; -.
DR EnsemblMetazoa; FBtr0075149; FBpp0074915; FBgn0000568. [P17671-1]
DR EnsemblMetazoa; FBtr0075151; FBpp0074917; FBgn0000568. [P17671-2]
DR GeneID; 39999; -.
DR CTD; 39999; -.
DR FlyBase; FBgn0000568; Eip75B.
DR VEuPathDB; VectorBase:FBgn0000568; -.
DR SignaLink; P17671; -.
DR BioGRID-ORCS; 39999; 1 hit in 3 CRISPR screens.
DR ChiTaRS; Eip75B; fly.
DR GenomeRNAi; 39999; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0000568; Expressed in brain and 27 other tissues.
DR ExpressionAtlas; P17671; baseline and differential.
DR Genevisible; P17671; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0020037; F:heme binding; IDA:FlyBase.
DR GO; GO:0004879; F:nuclear receptor activity; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0018990; P:ecdysis, chitin-based cuticle; IMP:FlyBase.
DR GO; GO:0009755; P:hormone-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0007591; P:molting cycle, chitin-based cuticle; IMP:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:FlyBase.
DR GO; GO:0048477; P:oogenesis; NAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007553; P:regulation of ecdysteroid metabolic process; IMP:FlyBase.
DR GO; GO:0010468; P:regulation of gene expression; IDA:FlyBase.
DR GO; GO:0035075; P:response to ecdysone; IMP:FlyBase.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001728; ThyrH_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR PRINTS; PR00546; THYROIDHORMR.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Metal-binding;
KW Nucleus; Receptor; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..1199
FT /note="Ecdysone-induced protein 75B, isoforms C/D"
FT /id="PRO_0000053506"
FT DOMAIN 352..600
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 242..318
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 245..265
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 282..306
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 130..182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 624..665
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 771..808
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 831..851
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 895..961
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 991..1104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1155..1188
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 130..151
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 626..665
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 776..807
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 831..849
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 897..919
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 934..961
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1155..1176
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..293
FT /note="Missing (in isoform D)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_014915"
FT VAR_SEQ 294..315
FT /note="NRNRCQYCRLKKCIAVGMSRDA -> MGEELPILKGILKGNVNYHNAP (in
FT isoform D)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_014916"
FT CONFLICT 239
FT /note="D -> G (in Ref. 4; ACD81870)"
FT /evidence="ECO:0000305"
FT CONFLICT 572
FT /note="T -> N (in Ref. 4; ACD81870)"
FT /evidence="ECO:0000305"
FT CONFLICT 578
FT /note="L -> P (in Ref. 4; ACD81870)"
FT /evidence="ECO:0000305"
FT CONFLICT 889
FT /note="H -> R (in Ref. 4; ACD81870)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1199 AA; 128534 MW; E29372F5E295F595 CRC64;
MLMSADSSDS AKTSVICSTV SASMLAPPAP EQPSTTAPPI LGVTGRSHLE NALKLPPNTS
VSAYYQHNSK LGMGQNYNPE FRSLVAPVTD LDTVPPTGVT MASSSNSPNS SVKLPHSGVI
FVSKSSAVST TDGPTAVLQQ QQPQQQMPQH FESLPHHHPQ QEHQPQQQQQ QHHLQHHPHP
HVMYPHGYQQ ANLHHSGGIA VVPADSRPQT PEYIKSYPVM DTTVASSVKG EPELNIEFDG
TTVLCRVCGD KASGFHYGVH SCEGCKGFFR RSIQQKIQYR PCTKNQQCSI LRINRNRCQY
CRLKKCIAVG MSRDAVRFGR VPKREKARIL AAMQQSTQNR GQQRALATEL DDQPRLLAAV
LRAHLETCEF TKEKVSAMRQ RARDCPSYSM PTLLACPLNP APELQSEQEF SQRFAHVIRG
VIDFAGMIPG FQLLTQDDKF TLLKAGLFDA LFVRLICMFD SSINSIICLN GQVMRRDAIQ
NGANARFLVD STFNFAERMN SMNLTDAEIG LFCAIVLITP DRPGLRNLEL IEKMYSRLKG
CLQYIVAQNR PDQPEFLAKL LETMPDLRTL STLHTEKLVV FRTEHKELLR QQMWSMEDGN
NSDGQQNKSP SGSWADAMDV EAAKSPLGSV SSTESADLDY GSPSSSQPQG VSLPSPPQQQ
PSALASSAPL LAATLSGGCP LRNRANSGSS GDSGAAEMDI VGSHAHLTQN GLTITPIVRH
QQQQQQQQQI GILNNAHSRN LNGGHAMCQQ QQQHPQLHHH LTAGAARYRK LDSPTDSGIE
SGNEKNECKA VSSGGSSSCS SPRSSVDDAL DCSDAAANHN QVVQHPQLSV VSVSPVRSPQ
PSTSSHLKRQ IVEDMPVLKR VLQAPPLYDT NSLMDEAYKP HKKFRALRHR EFETAEADAS
SSTSGSNSLS AGSPRQSPVP NSVATPPPSA ASAAAGNPAQ SQLHMHLTRS SPKASMASSH
SVLAKSLMAE PRMTPEQMKR SDIIQNYLKR ENSTAASSTT NGVGNRSPSS SSTPPPSAVQ
NQQRWGSSSV ITTTCQQRQQ SVSPHSNGSS SSSSSSSSSS SSSSSTSSNC SSSSASSCQY
FQSPHSTSNG TSAPASSSSG SNSATPLLEL QVDIADSAQP LNLSKKSPTP PPSKLHALVA
AANAVQRYPT LSADVTVTAS NGGPPSAAAS PAPSSSPPAS VGSPNPGLSA AVHKVMLEA
