E78C_DROME
ID E78C_DROME Reviewed; 862 AA.
AC P45447; O18395; O18396; Q7KTW8; Q8IPT7; Q9NBW8; Q9NBW9; Q9VP64;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 14-MAY-2014, sequence version 4.
DT 03-AUG-2022, entry version 190.
DE RecName: Full=Ecdysone-induced protein 78C;
DE Short=DR-78;
DE AltName: Full=Nuclear receptor subfamily 1 group E member 1;
GN Name=Eip78C; Synonyms=NR1E1; ORFNames=CG18023;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), FUNCTION, AND INDUCTION.
RC STRAIN=Canton-S; TISSUE=Larva, and Pupae;
RX PubMed=8402914; DOI=10.1016/0092-8674(93)80072-m;
RA Stone B.L., Thummel C.S.;
RT "The Drosophila 78C early late puff contains E78, an ecdysone-inducible
RT gene that encodes a novel member of the nuclear hormone receptor
RT superfamily.";
RL Cell 75:307-320(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), AND FUNCTION.
RC STRAIN=Canton-S; TISSUE=Pupae;
RX PubMed=8878682; DOI=10.1093/genetics/144.1.159;
RA Russell S.R.H., Heimbeck G., Goddard C.M., Carpenter A.T.C., Ashburner M.;
RT "The Drosophila Eip78C gene is not vital but has a role in regulating
RT chromosome puffs.";
RL Genetics 144:159-170(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
RA Nairz K., Hafen E.;
RL Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 318-429 (ISOFORM A).
RX PubMed=8241283; DOI=10.1016/0167-4781(93)90170-i;
RA Martin-Blanco E., Kornberg T.B.;
RT "DR-78, a novel Drosophila melanogaster genomic DNA fragment highly
RT homologous to the DNA-binding domain of thyroid hormone-retinoic acid-
RT vitamin D receptor subfamily.";
RL Biochim. Biophys. Acta 1216:339-341(1993).
CC -!- FUNCTION: Induces the early late puff 78C which triggers puparium
CC formation and development. {ECO:0000269|PubMed:8402914,
CC ECO:0000269|PubMed:8878682}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A; Synonyms=E78A;
CC IsoId=P45447-1; Sequence=Displayed;
CC Name=B; Synonyms=E78B;
CC IsoId=P45447-2; Sequence=VSP_003654;
CC -!- DEVELOPMENTAL STAGE: Isoform A is expressed only in mid-pupal stages,
CC while isoform B is maximally expressed in newly formed prepupae and
CC immediately following isoform A in mid-pupae.
CC -!- INDUCTION: Isoform A and isoform B require ecdysone for activity.
CC Isoform B also requires ecdysone-induced proteins for maximal
CC expression. {ECO:0000269|PubMed:8402914}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR1
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA19975.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAA19976.2; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA51523.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U01087; AAA19975.1; ALT_FRAME; mRNA.
DR EMBL; U01088; AAA19976.2; ALT_FRAME; mRNA.
DR EMBL; X98881; CAA67384.1; -; mRNA.
DR EMBL; X98882; CAA67385.1; -; mRNA.
DR EMBL; AF238308; AAF69494.1; -; mRNA.
DR EMBL; AF238309; AAF69495.1; -; mRNA.
DR EMBL; AE014296; AAF51692.2; -; Genomic_DNA.
DR EMBL; AE014296; AAN12161.3; -; Genomic_DNA.
DR EMBL; X73045; CAA51523.1; ALT_FRAME; Genomic_DNA.
DR PIR; A49070; A49070.
DR PIR; B49070; B49070.
DR PIR; S43435; S43435.
DR RefSeq; NP_001246862.1; NM_001259933.1. [P45447-1]
DR RefSeq; NP_001246863.1; NM_001259934.1. [P45447-1]
DR RefSeq; NP_524195.2; NM_079471.3. [P45447-1]
DR RefSeq; NP_730609.3; NM_168892.3. [P45447-2]
DR AlphaFoldDB; P45447; -.
DR SMR; P45447; -.
DR BioGRID; 65593; 57.
DR IntAct; P45447; 52.
DR STRING; 7227.FBpp0297287; -.
DR PaxDb; P45447; -.
DR EnsemblMetazoa; FBtr0078306; FBpp0077963; FBgn0004865. [P45447-1]
DR EnsemblMetazoa; FBtr0078307; FBpp0077964; FBgn0004865. [P45447-2]
DR EnsemblMetazoa; FBtr0306157; FBpp0297287; FBgn0004865. [P45447-1]
DR EnsemblMetazoa; FBtr0306158; FBpp0297288; FBgn0004865. [P45447-1]
DR GeneID; 40345; -.
DR KEGG; dme:Dmel_CG18023; -.
DR CTD; 40345; -.
DR FlyBase; FBgn0004865; Eip78C.
DR VEuPathDB; VectorBase:FBgn0004865; -.
DR eggNOG; KOG3575; Eukaryota.
DR eggNOG; KOG4846; Eukaryota.
DR HOGENOM; CLU_007368_2_2_1; -.
DR InParanoid; P45447; -.
DR OMA; PANTLGC; -.
DR PhylomeDB; P45447; -.
DR Reactome; R-DME-383280; Nuclear Receptor transcription pathway.
DR Reactome; R-DME-4090294; SUMOylation of intracellular receptors.
DR SignaLink; P45447; -.
DR BioGRID-ORCS; 40345; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40345; -.
DR PRO; PR:P45447; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0004865; Expressed in saliva-secreting gland and 13 other tissues.
DR ExpressionAtlas; P45447; baseline and differential.
DR Genevisible; P45447; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004879; F:nuclear receptor activity; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus; Receptor;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..862
FT /note="Ecdysone-induced protein 78C"
FT /id="PRO_0000053511"
FT DOMAIN 626..855
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 360..435
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 363..383
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 399..418
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 28..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 97..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 173..210
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 230..353
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..557
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 50..83
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..117
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 119..138
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 461..557
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 2..471
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:8402914,
FT ECO:0000303|PubMed:8878682, ECO:0000303|Ref.3"
FT /id="VSP_003654"
FT CONFLICT 39..40
FT /note="ED -> DH (in Ref. 2; CAA67384)"
FT /evidence="ECO:0000305"
FT CONFLICT 77
FT /note="D -> DEEED (in Ref. 1; AAA19975, 2; CAA67384 and 3;
FT AAF69494)"
FT /evidence="ECO:0000305"
FT CONFLICT 321..322
FT /note="QQ -> HE (in Ref. 2; CAA67384)"
FT /evidence="ECO:0000305"
FT CONFLICT 426
FT /note="A -> V (in Ref. 6; CAA51523)"
FT /evidence="ECO:0000305"
FT CONFLICT 429
FT /note="S -> K (in Ref. 6; CAA51523)"
FT /evidence="ECO:0000305"
FT CONFLICT 483..489
FT /note="Missing (in Ref. 3; AAF69495)"
FT /evidence="ECO:0000305"
FT CONFLICT 498
FT /note="Q -> P (in Ref. 1; AAA19975, 2; CAA67384 and 3;
FT AAF69494)"
FT /evidence="ECO:0000305"
FT CONFLICT 511
FT /note="Missing (in Ref. 2; CAA67384/CAA67385)"
FT /evidence="ECO:0000305"
FT CONFLICT 731
FT /note="Missing (in Ref. 1; AAA19975/AAA19976)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 862 AA; 94747 MW; EAD6ABC14882020F CRC64;
MDVYQIELEE QAQIRSKLLV ETCVKHSSSE QQQLQVKQED LIKDFTRDEE EQPSEEEAEE
EDNEEDEEEE GEEEEEDEDE EALLPVVNFN ANSDFNLHFF DTPEDSSTQG AYSEANSLES
EQEEEKQTQQ HQQQKQHHRD LEDCLSAIEA DPLQLLHCDD FYRTSALAES VAASLSPQQQ
QQRQHTHQQQ QQQQQQQQHP GQQQHQLNCT LSNGGGALYT ISSVHQFGPA SNHNTSSSSP
SSSAAHSSPD SGCSSASSSG SSRSCGSSSA SSSSSAVSST ISSGRSSNNS VVNPAATSSS
VAHLNKEQQQ QPLPTTQLQQ QQQHQQQLQH PQQQQSFGLA DSSSSNGSSN NNNGVSSKSF
VPCKVCGDKA SGYHYGVTSC EGCKGFFRRS IQKQIEYRCL RDGKCLVIRL NRNRCQYCRF
KKCLSAGMSR DSVRYGRVPK RSRELNGAAA SSAAAGAPAS LNVDDSTSST LHPSHLQQQQ
QQHLLQQQQQ QQHQPQLQQH HQLQQQPHVS GVRVKTPSTP QTPQMCSIAS SPSELGGCNS
ANNNNNNNNN SSSGNASGGS GVSVGVVVVG GHQQLVGGSM VGMAGMGTDA HQVGMCHDGL
AGTANELTVY DVIMCVSQAH RLNCSYTEEL TRELMRRPVT VPQNGIASTV AESLEFQKIW
LWQQFSARVT PGVQRIVEFA KRVPGFCDFT QDDQLILIKL GFFEVWLTHV ARLINEATLT
LDDGAYLTRQ QLEILYDSDF VNALLNFANT LNAYGLSDTE IGLFSAMVLL ASDRAGLSEP
KVIGRARELV AEALRVQILR SRAGSPQALQ LMPALEAKIP ELRSLGAKHF SHLDWLRMNW
TKLRLPPLFA EIFDIPKADD EL
