ID   E7_VACCW                Reviewed;         166 AA.
AC   P68446; P21048; Q76ZV8;
DT   23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   23-FEB-2022, entry version 56.
DE   RecName: Full=Protein E7;
GN   OrderedLocusNames=VACWR063; ORFNames=E7R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2398897; DOI=10.1128/mcb.10.10.5433-5441.1990;
RA   Ahn B.-Y., Gershon P.D., Jones E.V., Moss B.;
RT   "Identification of rpo30, a vaccinia virus RNA polymerase gene with
RT   structural similarity to a eucaryotic transcription elongation factor.";
RL   Mol. Cell. Biol. 10:5433-5441(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3012524; DOI=10.1073/pnas.83.11.3659;
RA   Earl P.L., Jones E.V., Moss B.;
RT   "Homology between DNA polymerases of poxviruses, herpesviruses, and
RT   adenoviruses: nucleotide sequence of the vaccinia virus DNA polymerase
RT   gene.";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:3659-3663(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   MYRISTOYLATION AT GLY-2, SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLY-2.
RX   PubMed=9188589; DOI=10.1128/jvi.71.7.5218-5226.1997;
RA   Martin K.H., Grosenbach D.W., Franke C.A., Hruby D.E.;
RT   "Identification and analysis of three myristylated vaccinia virus late
RT   proteins.";
RL   J. Virol. 71:5218-5226(1997).
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305|PubMed:9188589}.
CC   -!- PTM: Myristoylated. {ECO:0000269|PubMed:9188589}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae E7 family. {ECO:0000305}.
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DR   EMBL; M36339; AAB59827.1; -; Genomic_DNA.
DR   EMBL; M13213; AAA98421.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89342.1; -; Genomic_DNA.
DR   RefSeq; YP_232945.1; NC_006998.1.
DR   ELM; P68446; -.
DR   IntAct; P68446; 1.
DR   MINT; P68446; -.
DR   iPTMnet; P68446; -.
DR   DNASU; 3707596; -.
DR   GeneID; 3707596; -.
DR   KEGG; vg:3707596; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR035345; E7R.
DR   Pfam; PF17467; E7R; 1.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Lipoprotein; Myristate; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed; by host"
FT   CHAIN           2..166
FT                   /note="Protein E7"
FT                   /id="PRO_0000099459"
FT   LIPID           2
FT                   /note="N-myristoyl glycine; by host"
FT                   /evidence="ECO:0000269|PubMed:9188589"
FT   MUTAGEN         2
FT                   /note="G->A: Complete loss of myristoylation."
FT                   /evidence="ECO:0000269|PubMed:9188589"
SQ   SEQUENCE   166 AA;  19512 MW;  0E527693710FD087 CRC64;
     MGTAATIQTP TKLMNKENAE MILEKIVDHI VMYISDESSD SENNPEYIDF RNRYEDYRSL
     IIKSDHEFVK LCKNHAEKSS PETQQMIIKH IYEQYLIPVS EVLLKPIMSM GDIITYNGCK
     DNEWMLEQLS TLNFNNLRTW NSCSIGNVTR LFYTFFSYLM KDKLNI