ID   E8_VACCW                Reviewed;         273 AA.
AC   P23372; Q76ZV7;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   23-FEB-2022, entry version 63.
DE   RecName: Full=Protein E8;
GN   OrderedLocusNames=VACWR064; ORFNames=E8R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2398897; DOI=10.1128/mcb.10.10.5433-5441.1990;
RA   Ahn B.-Y., Gershon P.D., Jones E.V., Moss B.;
RT   "Identification of rpo30, a vaccinia virus RNA polymerase gene with
RT   structural similarity to a eucaryotic transcription elongation factor.";
RL   Mol. Cell. Biol. 10:5433-5441(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=11452001; DOI=10.1091/mbc.12.7.2031;
RA   Tolonen N., Doglio L., Schleich S., Krijnse Locker J.;
RT   "Vaccinia virus DNA replication occurs in endoplasmic reticulum-enclosed
RT   cytoplasmic mini-nuclei.";
RL   Mol. Biol. Cell 12:2031-2046(2001).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING, INDUCTION, AND PHOSPHORYLATION
RP   BY F10L.
RX   PubMed=12208956; DOI=10.1128/jvi.76.19.9773-9786.2002;
RA   Doglio L., De Marco A., Schleich S., Roos N., Krijnse Locker J.;
RT   "The Vaccinia virus E8R gene product: a viral membrane protein that is made
RT   early in infection and packaged into the virions' core.";
RL   J. Virol. 76:9773-9786(2002).
RN   [5]
RP   REVIEW.
RX   PubMed=16138898; DOI=10.1111/j.1600-0854.2005.00324.x;
RA   Schramm B., Locker J.K.;
RT   "Cytoplasmic organization of POXvirus DNA replication.";
RL   Traffic 6:839-846(2005).
CC   -!- FUNCTION: Early protein packaged into virion cores. May play a role in
CC       the biogenesis of the viral factories by recruiting and wrapping these
CC       DNA replication sites in endoplasmic reticulum derived membranes. Later
CC       in infection, phosphorylation of E8R by the late viral kinase F10L
CC       might decrease E8R DNA-binding ability and trigger ER membranes
CC       disassembly. Binds DNA in vitro. {ECO:0000269|PubMed:12208956}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host endoplasmic reticulum membrane
CC       {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Host
CC       cytoplasm. Note=Localizes to the inside membrane of cytoplasmic virus
CC       factories. Component of the core of mature virions.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:12208956}.
CC   -!- PTM: Phosphorylated by F10L kinase in vitro.
CC       {ECO:0000269|PubMed:12208956}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae E8 family. {ECO:0000305}.
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DR   EMBL; M36339; AAB59828.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89343.1; -; Genomic_DNA.
DR   RefSeq; YP_232946.1; NC_006998.1.
DR   DIP; DIP-2179N; -.
DR   IntAct; P23372; 1.
DR   MINT; P23372; -.
DR   DNASU; 3707597; -.
DR   GeneID; 3707597; -.
DR   KEGG; vg:3707597; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR005057; Poxvirus_E8.
DR   Pfam; PF03394; Pox_E8; 1.
DR   PIRSF; PIRSF015690; VAC_E8R; 1.
PE   1: Evidence at protein level;
KW   Early protein; Host cytoplasm; Host endoplasmic reticulum; Host membrane;
KW   Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW   Transmembrane helix; Virion.
FT   CHAIN           1..273
FT                   /note="Protein E8"
FT                   /id="PRO_0000099462"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        238..260
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   273 AA;  31889 MW;  6C274DCEE6353629 CRC64;
     MAATVPRFDD VYKNAQRRIL DQETFFSRGL SRPLMKNTYL FDNYAYGWIP ETAIWSSRYA
     NLDASDYYPI SLGLLKKFEF LMSLYKGPIP VYEEKVNTEF IANGSFSGRY VSYLRKFSAL
     PTNEFISFLL LTSIPIYNIL FWFKNTQFDI TKHTLFRYVY TDNAKHLALA RYMHQTGDYK
     PLFSRLKENY IFTGPVPIGI KDINHPNLSR ARSPSDYETL ANISTILYFT KYDPVLMFLL
     FYVPGYSITT KITPAVEYLM DKLNLTKSDV QLL