ID   E8_VAR67                Reviewed;         273 AA.
AC   P0DSY7; P33820;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   29-SEP-2021, entry version 6.
DE   RecName: Full=Protein E8;
GN   ORFNames=E8R;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8109158; DOI=10.1016/0168-1702(93)90093-3;
RA   Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V.,
RA   Sandakhchiev L.S.;
RT   "Analysis of the nucleotide sequence of a 43 kbp segment of the genome of
RT   variola virus India-1967 strain.";
RL   Virus Res. 30:239-258(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Early protein packaged into virion cores. May play a role in
CC       the biogenesis of the viral factories by recruiting and wrapping these
CC       DNA replication sites in endoplasmic reticulum derived membranes. Later
CC       in infection, phosphorylation of E8R by the late viral kinase F10L
CC       might decrease E8R DNA-binding ability and trigger ER membranes
CC       disassembly. Binds DNA in vitro (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host endoplasmic reticulum
CC       membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Host
CC       cytoplasm. Note=Localizes to the inside membrane of cytoplasmic virus
CC       factories. Component of the core of mature virions (MV) (By
CC       similarity). {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC   -!- PTM: Phosphorylated by F10L kinase in vitro. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae E8 family. {ECO:0000305}.
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DR   EMBL; X69198; CAA48990.1; -; Genomic_DNA.
DR   PIR; B36842; B36842.
DR   RefSeq; NP_042093.1; NC_001611.1.
DR   GeneID; 1486414; -.
DR   KEGG; vg:1486414; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR005057; Poxvirus_E8.
DR   Pfam; PF03394; Pox_E8; 1.
DR   PIRSF; PIRSF015690; VAC_E8R; 1.
PE   2: Evidence at transcript level;
KW   Early protein; Host cytoplasm; Host endoplasmic reticulum; Host membrane;
KW   Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW   Transmembrane helix; Virion.
FT   CHAIN           1..273
FT                   /note="Protein E8"
FT                   /id="PRO_0000099463"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..243
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   273 AA;  31898 MW;  02859D3F756A53AE CRC64;
     MAAVVPRFDD VYKNAQRRIL DQETFFSRGL GRPLMKNTYL FDNYAYGWIP ETAIWSSRYA
     NLDASDYYPI SLGLLKKFKF LMSLYKGPIP VYEEKVNTEF IANGSFSGRY VSYLRKFSAL
     PTNEFISFLL LTSIPIYNIL FWFKNTQFDI TKHTLFRYVY TDNAKHLALA RYMHQTGDYK
     PLFSRLKENY IFTGPVPIGI KDIDHPNLSR ARSPSDYETL ANISTILYFT KYDPVLMFLL
     FYVPGYSITT KITPAVEYLM DKLKLTKNDV QLL