ID   EA1_MAIZE               Reviewed;          94 AA.
AC   Q5G8Z3;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Protein EGG APPARATUS-1;
DE   AltName: Full=ZmEA1;
GN   Name=Ea1;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND TISSUE
RP   SPECIFICITY.
RC   STRAIN=cv. A188; TISSUE=Egg;
RX   PubMed=15681383; DOI=10.1126/science.1104954;
RA   Marton M.L., Cordts S., Broadhvest J., Dresselhaus T.;
RT   "Micropylar pollen tube guidance by egg apparatus 1 of maize.";
RL   Science 307:573-576(2005).
CC   -!- FUNCTION: Involved in short-range signaling required for pollen tube
CC       attraction by the female gametophyte. Required for female fertility.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC       membrane protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed only in the egg apparatus, consisting of
CC       the egg cell and two synergids. Not detected in the central cell,
CC       antipodals, and nucellar and integumental cells.
CC       {ECO:0000269|PubMed:15681383}.
CC   -!- DEVELOPMENTAL STAGE: Exclusively expressed in the mature egg cells
CC       before fertilization. {ECO:0000269|PubMed:15681383}.
CC   -!- INDUCTION: Rapid down-regulation and/or degradation after
CC       fertilization.
CC   -!- PTM: Possible proteolysis of the C-terminal region from the predicted
CC       transmembrane domain to permit secretion and transport of the mature
CC       protein to the cell walls of the nucellus, allowing the spreading from
CC       the egg cell apparatus to the micropylar opening of the ovule.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=Flower power - Issue 57 of
CC       April 2005;
CC       URL="https://web.expasy.org/spotlight/back_issues/057";
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DR   EMBL; AY733074; AAW58117.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5G8Z3; -.
DR   STRING; 4577.GRMZM2G456746_P01; -.
DR   PaxDb; Q5G8Z3; -.
DR   MaizeGDB; 936188; -.
DR   eggNOG; ENOG502R4NJ; Eukaryota.
DR   Proteomes; UP000007305; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR039926; Egg_app_1.
DR   PANTHER; PTHR33333; PTHR33333; 1.
PE   2: Evidence at transcript level;
KW   Fertilization; Membrane; Reference proteome; Signal-anchor; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..94
FT                   /note="Protein EGG APPARATUS-1"
FT                   /id="PRO_0000086887"
FT   TOPO_DOM        1..15
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        16..36
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        37..94
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   94 AA;  9470 MW;  901BC2287EEB33F4 CRC64;
     MSSCPAIVNM KDDDGIGAMG AAVAFAAMGV FGIYFLWPVV GPTSAGMMMK APGAAGWVIC
     RAVFEANPQL YFTILRTAGA AAAAATFAAC SIAS