ID   EA30_VICFA              Reviewed;         268 AA.
AC   P21745;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Embryonic abundant protein VF30.1;
DE   Flags: Precursor;
OS   Vicia faba (Broad bean) (Faba vulgaris).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Vicia.
OX   NCBI_TaxID=3906;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, AND
RP   GENE FAMILY.
RC   STRAIN=cv. Fribo; TISSUE=Seed;
RX   PubMed=24272345; AGRICOLA=IND92000056; DOI=10.1007/BF00027389;
RA   Bassuener R., Baeumlein H., Huth A., Jung R., Wobus U., Rapoport T.A.,
RA   Saalbach G., Muentz K.;
RT   "Abundant embryonic mRNA in field bean (Vicia faba L.) codes for a new
RT   class of seed proteins: cDNA cloning and characterization of the primary
RT   translation product.";
RL   Plant Mol. Biol. 11:321-334(1988).
RN   [2]
RP   GENE FAMILY.
RC   STRAIN=cv. Fribo; TISSUE=Leaf;
RX   PubMed=2017140; DOI=10.1007/bf00261688;
RA   Baumlein H., Boerjan W., Nagy I., Bassuner R., Van Montagu M., Inze D.,
RA   Wobus U.;
RT   "A novel seed protein gene from Vicia faba is developmentally regulated in
RT   transgenic tobacco and Arabidopsis plants.";
RL   Mol. Gen. Genet. 225:459-467(1991).
RN   [3]
RP   DOMAIN.
RX   PubMed=9790599; DOI=10.1007/s004380050832;
RA   Hattori J., Boutilier K.A., van Lookeren Campagne M.M., Miki B.L.;
RT   "A conserved BURP domain defines a novel group of plant proteins with
RT   unusual primary structures.";
RL   Mol. Gen. Genet. 259:424-428(1998).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24272345}.
CC       Note=Translocated in vitro across the endoplasmic reticulum membrane
CC       with concomitant removal of its signal peptide.
CC       {ECO:0000269|PubMed:24272345}.
CC   -!- TISSUE SPECIFICITY: Seed. {ECO:0000305|PubMed:24272345}.
CC   -!- DEVELOPMENTAL STAGE: Most abundant mRNA species from cotyledons at
CC       early stages of development, but the encoded protein does not
CC       accumulate in cotyledons. {ECO:0000269|PubMed:24272345}.
CC   -!- DOMAIN: The BURP domain located at the C-terminus has not been
CC       identified in non-plant proteins. {ECO:0000269|PubMed:9790599}.
CC   -!- MISCELLANEOUS: The USP gene family consists of roughly 10 to 20
CC       members. {ECO:0000269|PubMed:2017140}.
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DR   EMBL; X13242; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; S05471; S05471.
DR   AlphaFoldDB; P21745; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR044816; BURP.
DR   InterPro; IPR004873; BURP_dom.
DR   PANTHER; PTHR31236; PTHR31236; 2.
DR   Pfam; PF03181; BURP; 1.
DR   SMART; SM01045; BURP; 1.
DR   PROSITE; PS51277; BURP; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Repeat; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..268
FT                   /note="Embryonic abundant protein VF30.1"
FT                   /id="PRO_0000017348"
FT   DOMAIN          68..259
FT                   /note="BURP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00604"
FT   CARBOHYD        259
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   268 AA;  29757 MW;  906E129979E4ECB7 CRC64;
     MEFAHLTVLS LFCLAFVGIT ATSSGEDYWQ SIWPNTPLPK TFSDLLIPSG KTNSLPIKSE
     ELKQYSTLFF EHDLHPGKNF ILGNTNSVGS IIRPFTKSRQ GVTDSIWLAN KEKQSLEDFC
     YSPTAIAEHK HCVSSLKSMI DQVISHFGST KIKAISSNFA PYQDQYVVED VKKVGDNAVM
     CHRLNFEKVV FNCHQVRDTT AYVVSLVASD GTKTKALTVC HHDTRGMNPE LLYEALEVTP
     GTVPVCHFIG NKAAAWVPNH TADNLCVM