EA6_ARATH
ID EA6_ARATH Reviewed; 478 AA.
AC Q06915;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Probable glucan endo-1,3-beta-glucosidase A6;
DE EC=3.2.1.39;
DE AltName: Full=(1->3)-beta-glucan endohydrolase;
DE Short=(1->3)-beta-glucanase;
DE AltName: Full=Anther-specific protein A6;
DE AltName: Full=Beta-1,3-endoglucanase;
DE AltName: Full=Protein MATERNAL EFFECT EMBRYO ARREST 48 {ECO:0000303|PubMed:15634699};
DE Flags: Precursor;
GN Name=A6 {ECO:0000303|PubMed:8281185};
GN Synonyms=MEE48 {ECO:0000303|PubMed:15634699}; OrderedLocusNames=At4g14080;
GN ORFNames=dl3080c;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=8281185; DOI=10.1046/j.1365-313x.1993.04061023.x;
RA Hird D.L., Worrall D., Hodge R., Smartt S., Paul W., Scott R.;
RT "The anther-specific protein encoded by the Brassica napus and Arabidopsis
RT thaliana A6 gene displays similarity to beta-1,3-glucanases.";
RL Plant J. 4:1023-1033(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=15634699; DOI=10.1242/dev.01595;
RA Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S.,
RA Capron A., Xie L.-F., Ye D., Sundaresan V.;
RT "Genetic and molecular identification of genes required for female
RT gametophyte development and function in Arabidopsis.";
RL Development 132:603-614(2005).
RN [7]
RP FUNCTION.
RX PubMed=21849515; DOI=10.1104/pp.111.179523;
RA Dobritsa A.A., Geanconteri A., Shrestha J., Carlson A., Kooyers N.,
RA Coerper D., Urbanczyk-Wochniak E., Bench B.J., Sumner L.W., Swanson R.,
RA Preuss D.;
RT "A large-scale genetic screen in Arabidopsis to identify genes involved in
RT pollen exine production.";
RL Plant Physiol. 157:947-970(2011).
CC -!- FUNCTION: Probable beta-1,3-glucanase that may be involved in the
CC degradation of callose walls around the microspore tetrad during pollen
CC development (Probable). May be required for pollen exine formation
CC (PubMed:21849515). {ECO:0000269|PubMed:21849515,
CC ECO:0000305|PubMed:8281185}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC beta-D-glucans.; EC=3.2.1.39;
CC -!- TISSUE SPECIFICITY: Anthers. {ECO:0000269|PubMed:8281185}.
CC -!- PTM: Contains two additional disulfide bonds, but it is unclear if they
CC are between the pairs Cys-409-Cys-416 and Cys-425-Cys-471 or between
CC the pairs Cys-409-Cys-471 and Cys-416-Cys-425.
CC {ECO:0000250|UniProtKB:Q94G86}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality due to division arrested at
CC one-cell zygotic stage. {ECO:0000269|PubMed:15634699}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR EMBL; X70409; CAA49853.1; -; Genomic_DNA.
DR EMBL; Z97335; CAB10187.1; -; Genomic_DNA.
DR EMBL; AL161538; CAB78450.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83368.1; -; Genomic_DNA.
DR EMBL; AY099580; AAM20432.1; -; mRNA.
DR EMBL; BT002150; AAN72161.1; -; mRNA.
DR PIR; S31906; S31906.
DR RefSeq; NP_193144.1; NM_117483.4.
DR AlphaFoldDB; Q06915; -.
DR SMR; Q06915; -.
DR STRING; 3702.AT4G14080.1; -.
DR CAZy; CBM43; Carbohydrate-Binding Module Family 43.
DR CAZy; GH17; Glycoside Hydrolase Family 17.
DR PaxDb; Q06915; -.
DR PRIDE; Q06915; -.
DR ProteomicsDB; 224708; -.
DR EnsemblPlants; AT4G14080.1; AT4G14080.1; AT4G14080.
DR GeneID; 827044; -.
DR Gramene; AT4G14080.1; AT4G14080.1; AT4G14080.
DR KEGG; ath:AT4G14080; -.
DR Araport; AT4G14080; -.
DR TAIR; locus:2129376; AT4G14080.
DR eggNOG; ENOG502QRGZ; Eukaryota.
DR HOGENOM; CLU_024953_3_4_1; -.
DR InParanoid; Q06915; -.
DR OMA; YYPQTMI; -.
DR OrthoDB; 966331at2759; -.
DR PhylomeDB; Q06915; -.
DR BioCyc; ARA:AT4G14080-MON; -.
DR PRO; PR:Q06915; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q06915; baseline and differential.
DR Genevisible; Q06915; AT.
DR GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0010584; P:pollen exine formation; IMP:TAIR.
DR InterPro; IPR000490; Glyco_hydro_17.
DR InterPro; IPR044965; Glyco_hydro_17_plant.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR012946; X8.
DR PANTHER; PTHR32227; PTHR32227; 1.
DR Pfam; PF00332; Glyco_hydro_17; 1.
DR Pfam; PF07983; X8; 1.
DR SMART; SM00768; X8; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycosidase; Hydrolase; Plant defense; Reference proteome;
KW Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..478
FT /note="Probable glucan endo-1,3-beta-glucosidase A6"
FT /id="PRO_0000011883"
FT ACT_SITE 135
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:O22317"
FT ACT_SITE 280
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:O22317"
FT DISULFID 390..453
FT /evidence="ECO:0000250|UniProtKB:Q94G86"
SQ SEQUENCE 478 AA; 53472 MW; 68EAE92974E8FA05 CRC64;
MSLLAFFLFT ILVFSSSCCS ATRFQGHRYM QRKTMLDLAS KIGINYGRRG NNLPSPYQSI
NFIKSIKAGH VKLYDADPES LTLLSQTNLY VTITVPNHQI TALSSNQTIA DEWVRTNILP
YYPQTQIRFV LVGNEILSYN SGNVSVNLVP AMRKIVNSLR LHGIHNIKVG TPLAMDSLRS
SFPPSNGTFR EEITGPVMLP LLKFLNGTNS YFFLNVHPYF RWSRNPMNTS LDFALFQGHS
TYTDPQTGLV YRNLLDQMLD SVLFAMTKLG YPHMRLAISE TGWPNFGDID ETGANILNAA
TYNRNLIKKM SASPPIGTPS RPGLPIPTFV FSLFNENQKS GSGTQRHWGI LHPDGSPIYD
VDFTGQTPLT GFNPLPKPTN NVPYKGQVWC VPVEGANETE LEETLRMACA QSNTTCAALA
PGRECYEPVS IYWHASYALN SYWAQFRNQS IQCFFNGLAH ETTTNPGNDR CKFPSVTL
