EAA5_MOUSE
ID EAA5_MOUSE Reviewed; 559 AA.
AC Q8JZR4; A2A8F0;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Excitatory amino acid transporter 5;
DE AltName: Full=Solute carrier family 1 member 7;
GN Name=Slc1a7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Retina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transports L-glutamate; the L-glutamate uptake is sodium- and
CC voltage-dependent and chloride-independent. Its associated chloride
CC conductance may participate in visual processing (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with the PDZ domains of DLG4. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the dicarboxylate/amino acid:cation symporter
CC (DAACS) (TC 2.A.23) family. SLC1A7 subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAM23593.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK044586; BAC31989.1; -; mRNA.
DR EMBL; AK044671; BAC32027.1; -; mRNA.
DR EMBL; AL611936; CAM23593.1; ALT_INIT; Genomic_DNA.
DR EMBL; BC030400; AAH30400.1; -; mRNA.
DR CCDS; CCDS18444.3; -.
DR RefSeq; NP_666367.2; NM_146255.2.
DR AlphaFoldDB; Q8JZR4; -.
DR SMR; Q8JZR4; -.
DR STRING; 10090.ENSMUSP00000102324; -.
DR GlyGen; Q8JZR4; 2 sites.
DR iPTMnet; Q8JZR4; -.
DR PhosphoSitePlus; Q8JZR4; -.
DR PaxDb; Q8JZR4; -.
DR PRIDE; Q8JZR4; -.
DR ProteomicsDB; 277750; -.
DR Antibodypedia; 46889; 142 antibodies from 22 providers.
DR DNASU; 242607; -.
DR Ensembl; ENSMUST00000106713; ENSMUSP00000102324; ENSMUSG00000008932.
DR GeneID; 242607; -.
DR KEGG; mmu:242607; -.
DR CTD; 6512; -.
DR MGI; MGI:2444087; Slc1a7.
DR VEuPathDB; HostDB:ENSMUSG00000008932; -.
DR eggNOG; KOG3787; Eukaryota.
DR GeneTree; ENSGT00940000156073; -.
DR InParanoid; Q8JZR4; -.
DR OrthoDB; 1184392at2759; -.
DR PhylomeDB; Q8JZR4; -.
DR TreeFam; TF315206; -.
DR Reactome; R-MMU-210500; Glutamate Neurotransmitter Release Cycle.
DR Reactome; R-MMU-425393; Transport of inorganic cations/anions and amino acids/oligopeptides.
DR BioGRID-ORCS; 242607; 2 hits in 75 CRISPR screens.
DR ChiTaRS; Slc1a7; mouse.
DR PRO; PR:Q8JZR4; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8JZR4; protein.
DR Bgee; ENSMUSG00000008932; Expressed in retinal neural layer and 17 other tissues.
DR ExpressionAtlas; Q8JZR4; baseline and differential.
DR GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR GO; GO:0099055; C:integral component of postsynaptic membrane; IDA:SynGO.
DR GO; GO:0099056; C:integral component of presynaptic membrane; IDA:SynGO.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0008509; F:anion transmembrane transporter activity; IDA:MGI.
DR GO; GO:0015501; F:glutamate:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0005313; F:L-glutamate transmembrane transporter activity; IDA:MGI.
DR GO; GO:0015175; F:neutral amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0098656; P:anion transmembrane transport; IDA:MGI.
DR GO; GO:0015813; P:L-glutamate transmembrane transport; IDA:MGI.
DR GO; GO:0098810; P:neurotransmitter reuptake; ISO:MGI.
DR GO; GO:0001504; P:neurotransmitter uptake; ISO:MGI.
DR Gene3D; 1.10.3860.10; -; 1.
DR InterPro; IPR001991; Na-dicarboxylate_symporter.
DR InterPro; IPR018107; Na-dicarboxylate_symporter_CS.
DR InterPro; IPR036458; Na:dicarbo_symporter_sf.
DR Pfam; PF00375; SDF; 1.
DR SUPFAM; SSF118215; SSF118215; 1.
DR PROSITE; PS00713; NA_DICARBOXYL_SYMP_1; 1.
DR PROSITE; PS00714; NA_DICARBOXYL_SYMP_2; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..559
FT /note="Excitatory amino acid transporter 5"
FT /id="PRO_0000284451"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..215
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 456..476
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 190
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 253
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 559 AA; 60106 MW; BEC713C884E27187 CRC64;
MVLDAVLARG RTVCKHNGLL ILSVLSVIVG CLLGFFLRTQ RLSPQEISYF QFPGELLMRM
LKMLILPLVV SSLMSGLASL DAKTSSRLGI LTVAYYLWTT FLAVVVGIIM VSIIHPGGAA
QKETTEQSGK PVMSSADALL DLVRNMFPAN LVEATFKQYR TKTTPVIKSP RGAAEEAPRR
IVIYGVQEDN GSRVQNFALD LTPPPEIVYK SEPGTSDGMN VLGIVIFSAT MGIMLGRMGD
SGTPLVSFCQ CLNESVMKIV AVAGWYFPFG IVFLIAGKIL EMDDPKAVGK KLGFYAVTVV
CGLVVHGLLI LPLLYFLITK KNPIVFIRGV LQALLIALAT SSSSATLPIT FKCLLENNHI
DRRIARFVLP VGATINMDGT ALYEAVAAIF IAQVNNYELD FGQIITISIT ATAASIGAAG
IPQAGLVTMV IVLTSVGLPT DDINLIIAVD WALDRFRTMI NVLGDALAAG IMAHICRKDF
AQDMGTEKLL PCETKPVTLQ EIVAAQQNGC VKSVAEASEL TLGPTCPHHI PVQVEQDEDP
AAASLDHCTI EISELETNV
