ID   EAE_CITFR               Reviewed;         936 AA.
AC   Q07591;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Intimin;
DE   AltName: Full=Attaching and effacing protein;
DE            Short=Eae protein;
GN   Name=eae;
OS   Citrobacter freundii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
OX   NCBI_TaxID=546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8500884; DOI=10.1128/iai.61.6.2486-2492.1993;
RA   Schauer D.B., Falkow S.;
RT   "Attaching and effacing locus of a Citrobacter freundii biotype that causes
RT   transmissible murine colonic hyperplasia.";
RL   Infect. Immun. 61:2486-2492(1993).
CC   -!- FUNCTION: Necessary for the production of attaching and effacing
CC       lesions on tissue culture cells. Believed to mediate adherence.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Single-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the intimin/invasin family. {ECO:0000305}.
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DR   EMBL; L11691; AAA23097.1; -; Genomic_DNA.
DR   PIR; I40705; I40705.
DR   AlphaFoldDB; Q07591; -.
DR   SMR; Q07591; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR   Gene3D; 2.40.160.160; -; 1.
DR   Gene3D; 2.60.40.10; -; 2.
DR   Gene3D; 3.10.100.10; -; 1.
DR   InterPro; IPR003344; Big_1_dom.
DR   InterPro; IPR003343; Big_2.
DR   InterPro; IPR016186; C-type_lectin-like/link_sf.
DR   InterPro; IPR016187; CTDL_fold.
DR   InterPro; IPR024519; IAT_beta.
DR   InterPro; IPR038177; IAT_beta_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003535; Intimin/invasin_bac.
DR   InterPro; IPR013117; Intimin_C.
DR   InterPro; IPR008964; Invasin/intimin_cell_adhesion.
DR   InterPro; IPR018392; LysM_dom.
DR   Pfam; PF02369; Big_1; 2.
DR   Pfam; PF02368; Big_2; 1.
DR   Pfam; PF11924; IAT_beta; 1.
DR   Pfam; PF07979; Intimin_C; 1.
DR   Pfam; PF01476; LysM; 1.
DR   PRINTS; PR01369; INTIMIN.
DR   SMART; SM00634; BID_1; 2.
DR   SMART; SM00635; BID_2; 1.
DR   SMART; SM00257; LysM; 1.
DR   SUPFAM; SSF49373; SSF49373; 3.
DR   SUPFAM; SSF56436; SSF56436; 1.
DR   PROSITE; PS51127; BIG1; 2.
DR   PROSITE; PS51782; LYSM; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Membrane; Repeat; Transmembrane; Transmembrane helix;
KW   Virulence.
FT   CHAIN           1..936
FT                   /note="Intimin"
FT                   /id="PRO_0000211825"
FT   TRANSMEM        20..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          63..112
FT                   /note="LysM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01118"
FT   DOMAIN          557..650
FT                   /note="Big-1 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00445"
FT   DOMAIN          657..748
FT                   /note="Big-1 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00445"
FT   DOMAIN          781..831
FT                   /note="BIG2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   936 AA;  102104 MW;  DC8929527D765EFA CRC64;
     MIIHGFCTGT RHKHKLRKTF IMLGAGLGLF FSVNQNSFAN GENYFKLRAD SKLINNNIAQ
     DRLFYTLKTG ESVAQLSKSQ GISVPVIWSL NKHLYSSESE MMKASPGQQI ILPLKKLSAE
     YSTLPILGTA PVVAAADVAG HTKKMSQDTT KSNTSDDKAL NYAAQQAASL GSQLQSRSLN
     GDYAKDTALS MAGNQASSQM QAWLQHYGTA EVNLQSGNNF DGSSLDFLLP FYDTENMLAF
     GQVGARYIDS RFTANLGAGQ RFFLPENMLG YNVFIDQDFS GNNTRLGIGG EYWRDYFKSS
     VNGYFRMSGW HESYNKKDYD ERPANGFDIR FNGYLPSYPA LGGKLMYEQY YGDNVALFNA
     DKLYSNPGAV TVGVNYTPIP LVTMGIDYRH GTGNENDLLY SMQFHYQFDK PWSQQIEPQY
     VNELRTLSGS RYDLVQRNNN IILDYKKQDI LSMNIPHNIN GTEHSTHKIQ LIVKSKYGLE
     RIVWDDSTLR TQGGQIQHSE RKAHNDYQAI LPAYVQGGSN VYKVTRRAYD RNGNSSNNVQ
     LTITVLSNGQ VVDKVGITNF TADKTSAKAD NSDTITYTAT VKKNGVAQAN VPVSFNIVSG
     TATLSAKSAN TNSSGKATVT LKSDKPGQVV VSAKTAEMTS ALNANAVIFV DQTKASITEI
     KVDKTIATAD NKDTIEYTVK VMKGGNPISG QKVTFSKDFG TLNKTEATTD QNGYATVKLS
     SGTPGKAIVS AKVSEVNTEV KAATVEFFAP LSIDGNKVTV IGTGVTGSLP KNWLQYGQVK
     LQATGGNGKY TWKSSNTKIA SVDNSGVITL NEKGSATITV VSGDNQSATY TINTPDNIII
     AVDKINRMAY SEAESRCQAI SSNLAQSKSV LENIYSKWGA ANKYPYYSSS NSLTAWIKQS
     TSDSASGVSN TYDLVTTNSL TNVKATDKNA FAVCVK