ID   EAGT6_PSEAE             Reviewed;         144 AA.
AC   Q9I738;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Effector EagT6 {ECO:0000303|PubMed:30177742};
GN   Name=eagT6 {ECO:0000303|PubMed:30177742}; OrderedLocusNames=PA0094;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, INTERACTION WITH TSE6, AND SUBUNIT.
RX   PubMed=30177742; DOI=10.1038/s41564-018-0238-z;
RA   Quentin D., Ahmad S., Shanthamoorthy P., Mougous J.D., Whitney J.C.,
RA   Raunser S.;
RT   "Mechanism of loading and translocation of type VI secretion system
RT   effector Tse6.";
RL   Nat. Microbiol. 3:1142-1152(2018).
RN   [3] {ECO:0007744|PDB:1TU1}
RP   X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
RA   Osipiuk J., Evdokimova E., Savchenko A., Edwards A., Cymborowski M.,
RA   Minor W., Joachimiak A.;
RT   "X-ray crystal structure of hypothetical protein PA94 from Pseudomonas
RT   aeruginosa.";
RL   Submitted (JUN-2004) to the PDB data bank.
CC   -!- FUNCTION: Plays an essential role in toxin Tse6 delivery to target
CC       cells and specifically in the loading of Tse6 onto VgrG1a.
CC       {ECO:0000269|PubMed:30177742}.
CC   -!- SUBUNIT: Homodimer. Two dimers interact with Tse6; this interaction is
CC       crucial for Tse6 loading onto VgrG1a. {ECO:0000269|PubMed:30177742}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE004091; AAG03484.1; -; Genomic_DNA.
DR   PIR; C83633; C83633.
DR   RefSeq; NP_248784.1; NC_002516.2.
DR   RefSeq; WP_003083692.1; NZ_QZGE01000015.1.
DR   PDB; 1TU1; X-ray; 1.95 A; A/B=1-144.
DR   PDB; 6XRF; X-ray; 2.56 A; A/B/D/E/G/H=1-144.
DR   PDBsum; 1TU1; -.
DR   PDBsum; 6XRF; -.
DR   AlphaFoldDB; Q9I738; -.
DR   SMR; Q9I738; -.
DR   STRING; 287.DR97_3051; -.
DR   PaxDb; Q9I738; -.
DR   PRIDE; Q9I738; -.
DR   DNASU; 879595; -.
DR   EnsemblBacteria; AAG03484; AAG03484; PA0094.
DR   GeneID; 879595; -.
DR   KEGG; pae:PA0094; -.
DR   PATRIC; fig|208964.12.peg.98; -.
DR   PseudoCAP; PA0094; -.
DR   HOGENOM; CLU_126902_2_0_6; -.
DR   OMA; QFAEREM; -.
DR   BioCyc; PAER208964:G1FZ6-96-MON; -.
DR   EvolutionaryTrace; Q9I738; -.
DR   Proteomes; UP000002438; Chromosome.
DR   InterPro; IPR014894; DcrB/EagT6.
DR   InterPro; IPR016123; Mog1/PsbP_a/b/a-sand.
DR   Pfam; PF08786; DcrB; 1.
DR   SUPFAM; SSF55724; SSF55724; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome.
FT   CHAIN           1..144
FT                   /note="Effector EagT6"
FT                   /id="PRO_0000449105"
FT   STRAND          1..5
FT                   /evidence="ECO:0007829|PDB:1TU1"
FT   STRAND          7..14
FT                   /evidence="ECO:0007829|PDB:1TU1"
FT   STRAND          17..19
FT                   /evidence="ECO:0007829|PDB:1TU1"
FT   STRAND          22..27
FT                   /evidence="ECO:0007829|PDB:1TU1"
FT   STRAND          36..43
FT                   /evidence="ECO:0007829|PDB:1TU1"
FT   HELIX           51..65
FT                   /evidence="ECO:0007829|PDB:1TU1"
FT   STRAND          70..79
FT                   /evidence="ECO:0007829|PDB:1TU1"
FT   STRAND          82..93
FT                   /evidence="ECO:0007829|PDB:1TU1"
FT   STRAND          96..105
FT                   /evidence="ECO:0007829|PDB:1TU1"
FT   STRAND          108..118
FT                   /evidence="ECO:0007829|PDB:1TU1"
FT   HELIX           120..122
FT                   /evidence="ECO:0007829|PDB:1TU1"
FT   HELIX           123..135
FT                   /evidence="ECO:0007829|PDB:1TU1"
SQ   SEQUENCE   144 AA;  16488 MW;  EC3ACBC54828157F CRC64;
     MTLYRLHEAD LEIPDAWQDQ SINIFKLPAS GPAREASFVI SRDASQGDAP FADYVARQLE
     NAEKQLPGFK LHKRWDINIH GHAAVLLDYQ WQREGRDLML RQVFIERRPA VLITTLTTTP
     ADLPHHEPAW KQAMQTLVPR PTPS