EAMA_ECOLI
ID EAMA_ECOLI Reviewed; 299 AA.
AC P31125;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Probable amino-acid metabolite efflux pump;
GN Name=eamA; Synonyms=ydeD; OrderedLocusNames=b1533, JW5250;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8383113; DOI=10.1128/jb.175.5.1484-1492.1993;
RA Cohen S.P., Haechler H., Levy S.B.;
RT "Genetic and functional analysis of the multiple antibiotic resistance
RT (mar) locus in Escherichia coli.";
RL J. Bacteriol. 175:1484-1492(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP IDENTIFICATION OF START CODON, AND FUNCTION.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=10844694; DOI=10.1046/j.1365-2958.2000.01924.x;
RA Dassler T., Maier T., Winterhalter C., Boeck A.;
RT "Identification of a major facilitator protein from Escherichia coli
RT involved in efflux of metabolites of the cysteine pathway.";
RL Mol. Microbiol. 36:1101-1112(2000).
RN [6]
RP FUNCTION.
RA Dassler T.;
RL Unpublished observations (JAN-2001).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: May be an export pump for cysteine and other metabolites of
CC the cysteine pathway (such as N-acetyl-L-serine (NAS) and O-acetyl-L-
CC serine (OAS)), and for other amino acids and their metabolites.
CC {ECO:0000269|PubMed:10844694, ECO:0000269|Ref.6}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the EamA transporter family. {ECO:0000305}.
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DR EMBL; M96235; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U00096; AAC74606.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15223.2; -; Genomic_DNA.
DR PIR; H64907; H64907.
DR RefSeq; NP_416050.4; NC_000913.3.
DR RefSeq; WP_000087187.1; NZ_LN832404.1.
DR AlphaFoldDB; P31125; -.
DR SMR; P31125; -.
DR BioGRID; 4259112; 4.
DR STRING; 511145.b1533; -.
DR TCDB; 2.A.7.3.2; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; P31125; -.
DR PRIDE; P31125; -.
DR EnsemblBacteria; AAC74606; AAC74606; b1533.
DR EnsemblBacteria; BAA15223; BAA15223; BAA15223.
DR GeneID; 946081; -.
DR KEGG; ecj:JW5250; -.
DR KEGG; eco:b1533; -.
DR PATRIC; fig|1411691.4.peg.733; -.
DR EchoBASE; EB1594; -.
DR eggNOG; COG0697; Bacteria.
DR HOGENOM; CLU_033863_20_1_6; -.
DR InParanoid; P31125; -.
DR OMA; NTLTWPV; -.
DR PhylomeDB; P31125; -.
DR BioCyc; EcoCyc:EG11639-MON; -.
DR BioCyc; MetaCyc:EG11639-MON; -.
DR PRO; PR:P31125; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015562; F:efflux transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0070301; P:cellular response to hydrogen peroxide; IEP:EcoCyc.
DR GO; GO:0033228; P:cysteine export across plasma membrane; IMP:EcoCyc.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 2.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..299
FT /note="Probable amino-acid metabolite efflux pump"
FT /id="PRO_0000108148"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..31
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..59
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..87
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..116
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..140
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..175
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..216
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 238..243
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 265..268
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 290..299
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 14..132
FT /note="EamA 1"
FT DOMAIN 153..287
FT /note="EamA 2"
SQ SEQUENCE 299 AA; 32324 MW; 5FC73EFD2C08D565 CRC64;
MSRKDGVLAL LVVVVWGLNF VVIKVGLHNM PPLMLAGLRF MLVAFPAIFF VARPKVPLNL
LLGYGLTISF AQFAFLFCAI NFGMPAGLAS LVLQAQAFFT IMLGAFTFGE RLHGKQLAGI
ALAIFGVLVL IEDSLNGQHV AMLGFMLTLA AAFSWACGNI FNKKIMSHST RPAVMSLVIW
SALIPIIPFF VASLILDGSA TMIHSLVTID MTTILSLMYL AFVATIVGYG IWGTLLGRYE
TWRVAPLSLL VPVVGLASAA LLLDERLTGL QFLGAVLIMT GLYINVFGLR WRKAVKVGS
