EAMA_SALTY
ID EAMA_SALTY Reviewed; 299 AA.
AC Q56072;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 19-DEC-2001, sequence version 2.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Probable amino-acid metabolite efflux pump;
GN Name=eamA; OrderedLocusNames=STM1517;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 250-299.
RC STRAIN=X3181;
RX PubMed=9068629; DOI=10.1128/jb.179.6.1857-1866.1997;
RA Sulavik M.C., Dazer M., Miller P.F.;
RT "The Salmonella typhimurium mar locus: molecular and genetic analyses and
RT assessment of its role in virulence.";
RL J. Bacteriol. 179:1857-1866(1997).
CC -!- FUNCTION: May be an export pump for several amino acids and their
CC metabolites, including cysteine. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the EamA transporter family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL20436.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE006468; AAL20436.1; ALT_INIT; Genomic_DNA.
DR EMBL; U54468; AAC44979.1; -; Genomic_DNA.
DR PIR; T11759; T11759.
DR RefSeq; NP_460477.1; NC_003197.2.
DR AlphaFoldDB; Q56072; -.
DR SMR; Q56072; -.
DR STRING; 99287.STM1517; -.
DR PaxDb; Q56072; -.
DR EnsemblBacteria; AAL20436; AAL20436; STM1517.
DR GeneID; 1253035; -.
DR KEGG; stm:STM1517; -.
DR PATRIC; fig|99287.12.peg.1605; -.
DR HOGENOM; CLU_033863_20_1_6; -.
DR OMA; NTLTWPV; -.
DR PhylomeDB; Q56072; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 2.
PE 3: Inferred from homology;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..299
FT /note="Probable amino-acid metabolite efflux pump"
FT /id="PRO_0000108149"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..31
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..59
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..87
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..116
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..139
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..175
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..216
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 238..243
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 265..268
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 290..299
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 14..132
FT /note="EamA 1"
FT DOMAIN 153..287
FT /note="EamA 2"
SQ SEQUENCE 299 AA; 32160 MW; 63E23CA4C91718E1 CRC64;
MSRKDGFLAL LVVVVWGLNF VVIKVGLHHM PPLLLAGLRF LLVAFPAIFF VARPKVPLTL
LLGYGLTISF GQFAFLFCAI KFGMPAGLAS LVLQAQAFFT MALGAFVFSE RLQRKQLAGI
ALAIIGVLVL IEASLNGQHI AMSGFMLTLA AAFSWACGNI FNKKIMQHSP RPAVMSLVVW
SALIPILPFL LSSLLLEGAD HITQSLITID MTTILSLLYL AFVATILGYG IWGALLGRYE
TWRVAPLSLL VPVVGLASAA VLLGETLTGM QLAGAVLIMA GLYINVFGFR VRRTARVSG
