ID   EAMB_ECOUT              Reviewed;         195 AA.
AC   Q1R8F4;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Cysteine/O-acetylserine efflux protein {ECO:0000250|UniProtKB:P38101};
GN   Name=eamB; OrderedLocusNames=UTI89_C2900;
OS   Escherichia coli (strain UTI89 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=364106;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTI89 / UPEC;
RX   PubMed=16585510; DOI=10.1073/pnas.0600938103;
RA   Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A.,
RA   Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S.,
RA   Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J.,
RA   Gordon J.I.;
RT   "Identification of genes subject to positive selection in uropathogenic
RT   strains of Escherichia coli: a comparative genomics approach.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006).
CC   -!- FUNCTION: Exporter of O-acetylserine (OAS) and cysteine.
CC       {ECO:0000250|UniProtKB:P38101}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=O-acetyl-L-serine(in) = O-acetyl-L-serine(out);
CC         Xref=Rhea:RHEA:29659, ChEBI:CHEBI:58340;
CC         Evidence={ECO:0000250|UniProtKB:P38101};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29660;
CC         Evidence={ECO:0000250|UniProtKB:P38101};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-cysteine(in) = L-cysteine(out); Xref=Rhea:RHEA:29655,
CC         ChEBI:CHEBI:35235; Evidence={ECO:0000250|UniProtKB:P38101};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29656;
CC         Evidence={ECO:0000250|UniProtKB:P38101};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P38101}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the Rht family. {ECO:0000305}.
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DR   EMBL; CP000243; ABE08360.1; -; Genomic_DNA.
DR   RefSeq; WP_000189209.1; NC_007946.1.
DR   AlphaFoldDB; Q1R8F4; -.
DR   EnsemblBacteria; ABE08360; ABE08360; UTI89_C2900.
DR   KEGG; eci:UTI89_C2900; -.
DR   HOGENOM; CLU_079569_1_2_6; -.
DR   OMA; NPKAWIM; -.
DR   Proteomes; UP000001952; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR001123; LeuE-type.
DR   PANTHER; PTHR30086; PTHR30086; 1.
DR   Pfam; PF01810; LysE; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..195
FT                   /note="Cysteine/O-acetylserine efflux protein"
FT                   /id="PRO_0000318720"
FT   TOPO_DOM        1..7
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        29..46
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        68..69
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        91..104
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        105..125
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        126..141
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        142..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        163..176
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        195
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P38101"
SQ   SEQUENCE   195 AA;  21278 MW;  295DD583ADCA8584 CRC64;
     MTPTLLSAFW TYTLITAMTP GPNNILALSS ATSHGFRQST RVLAGMSLGF LIVMLLCAGI
     SFSLAVIDPA AVHLLSWAGA AYIVWLAWKI ATSPTKEDGL QTKPISFWAS FALQFVNVKI
     ILYGVTALST FVLPQTQALS WVVGVSVLLA MIGTFGNVCW ALAGHLFQRL FRQYGRQLNI
     VLALLLVYCA VRIFY