EAPP_MOUSE
ID EAPP_MOUSE Reviewed; 281 AA.
AC Q5BU09; Q9CZB5; Q9D914;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=E2F-associated phosphoprotein;
DE Short=EAPP;
GN Name=Eapp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND INTERACTION WITH E2F1; E2F2 AND
RP E2F3.
RX PubMed=15716352; DOI=10.1091/mbc.e04-11-0975;
RA Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L.,
RA Rotheneder H.;
RT "EAPP, a novel E2F binding protein that modulates E2F-dependent
RT transcription.";
RL Mol. Biol. Cell 16:2181-2190(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N-3; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109 AND SER-111, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play an important role in the fine-tuning of both major
CC E2F1 activities, the regulation of the cell-cycle and the induction of
CC apoptosis. Promotes S-phase entry, and inhibits p14(ARP) expression (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with E2F1. The C-terminal half binds the N-terminal
CC of E2F1. Also interacts with E2F2 and E2F3, but not E2F4.
CC {ECO:0000269|PubMed:15716352}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5BU09-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5BU09-2; Sequence=VSP_015331;
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DR EMBL; AY882557; AAX20161.1; -; mRNA.
DR EMBL; AK007442; BAB25042.1; -; mRNA.
DR EMBL; AK012800; BAB28479.1; -; mRNA.
DR EMBL; BC037622; AAH37622.1; -; mRNA.
DR CCDS; CCDS25910.1; -. [Q5BU09-1]
DR CCDS; CCDS88337.1; -. [Q5BU09-2]
DR RefSeq; NP_001318091.1; NM_001331162.1. [Q5BU09-2]
DR RefSeq; NP_079732.1; NM_025456.4. [Q5BU09-1]
DR RefSeq; XP_006516205.1; XM_006516142.3. [Q5BU09-1]
DR AlphaFoldDB; Q5BU09; -.
DR STRING; 10090.ENSMUSP00000123698; -.
DR iPTMnet; Q5BU09; -.
DR PhosphoSitePlus; Q5BU09; -.
DR EPD; Q5BU09; -.
DR MaxQB; Q5BU09; -.
DR PaxDb; Q5BU09; -.
DR PRIDE; Q5BU09; -.
DR ProteomicsDB; 277710; -. [Q5BU09-1]
DR ProteomicsDB; 277711; -. [Q5BU09-2]
DR Antibodypedia; 128; 134 antibodies from 26 providers.
DR DNASU; 66266; -.
DR Ensembl; ENSMUST00000110713; ENSMUSP00000106341; ENSMUSG00000054302. [Q5BU09-2]
DR Ensembl; ENSMUST00000161592; ENSMUSP00000123698; ENSMUSG00000054302. [Q5BU09-1]
DR Ensembl; ENSMUST00000163433; ENSMUSP00000130251; ENSMUSG00000054302. [Q5BU09-1]
DR GeneID; 66266; -.
DR KEGG; mmu:66266; -.
DR UCSC; uc007nnu.1; mouse. [Q5BU09-1]
DR UCSC; uc011ylx.1; mouse. [Q5BU09-2]
DR CTD; 55837; -.
DR MGI; MGI:1913516; Eapp.
DR VEuPathDB; HostDB:ENSMUSG00000054302; -.
DR eggNOG; KOG3395; Eukaryota.
DR GeneTree; ENSGT00390000001332; -.
DR HOGENOM; CLU_075202_0_0_1; -.
DR InParanoid; Q5BU09; -.
DR OMA; MEGTWAP; -.
DR OrthoDB; 1484224at2759; -.
DR PhylomeDB; Q5BU09; -.
DR TreeFam; TF328497; -.
DR BioGRID-ORCS; 66266; 18 hits in 71 CRISPR screens.
DR ChiTaRS; Eapp; mouse.
DR PRO; PR:Q5BU09; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q5BU09; protein.
DR Bgee; ENSMUSG00000054302; Expressed in interventricular septum and 255 other tissues.
DR ExpressionAtlas; Q5BU09; baseline and differential.
DR Genevisible; Q5BU09; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; ISO:MGI.
DR InterPro; IPR019370; E2F-assoc_phosphoprotein.
DR PANTHER; PTHR15967; PTHR15967; 1.
DR Pfam; PF10238; Eapp_C; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cytoplasm; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..281
FT /note="E2F-associated phosphoprotein"
FT /id="PRO_0000086905"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 222..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..26
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q56P03"
FT MOD_RES 17
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q56P03"
FT MOD_RES 37
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q56P03"
FT MOD_RES 109
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 111
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 156..193
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_015331"
FT CONFLICT 116
FT /note="K -> R (in Ref. 1; AAX20161)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 281 AA; 32494 MW; 269C664CA3E3C401 CRC64;
MNRLQDDYDP YAVEEPSDEE PALSSSEDEL DVLLHGTPDQ KRKLIRECLT GESESSSEDE
FEKEMEAELN STMKTMEDQL SSLGTGSSSG VAKVGGVTEK FYDEIYFDSD SEDEDKTVTK
KKKKKQHRIP TNDELLYDPE KDNRDQAWVD AKRRGYHAFG LQRPRQKQQP VPNSDAVLNC
PACMTTLCLD CQRHESYKTQ YRAMFVMNCS INREEVLRYK NPENRRKRRS AKKMRSNPED
PAEREAEEIY HPVMCTECST EVAVYDKDEV FHFFNVLASH S
