ADPGK_HUMAN
ID ADPGK_HUMAN Reviewed; 497 AA.
AC Q9BRR6; Q49AU7; Q8NBI1; Q8WZ90; Q96NF8; Q9H0A7;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=ADP-dependent glucokinase;
DE Short=ADP-GK;
DE Short=ADPGK;
DE EC=2.7.1.147;
DE AltName: Full=RbBP-35;
DE Flags: Precursor;
GN Name=ADPGK; ORFNames=PSEC0260;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
RC TISSUE=Lymph node;
RA Fan Z.S., Ao S.Z.;
RT "RbBP-35 interacted with pRb.";
RL Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Testis;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Heart;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Teratocarcinoma;
RX PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA Isogai T.;
RT "Signal sequence and keyword trap in silico for selection of full-length
RT human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA
RT libraries.";
RL DNA Res. 12:117-126(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 6).
RC TISSUE=Brain, and Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: Catalyzes the phosphorylation of D-glucose to D-glucose 6-
CC phosphate using ADP as the phosphate donor. GDP and CDP can replace
CC ADP, but with reduced efficiency (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ADP + D-glucose = AMP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:11460, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:61548, ChEBI:CHEBI:456215, ChEBI:CHEBI:456216;
CC EC=2.7.1.147;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00584};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|PROSITE-
CC ProRule:PRU00584};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis. {ECO:0000255|PROSITE-
CC ProRule:PRU00584}.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Name=1;
CC IsoId=Q9BRR6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BRR6-2; Sequence=VSP_013552;
CC Name=3;
CC IsoId=Q9BRR6-3; Sequence=VSP_013549;
CC Name=4;
CC IsoId=Q9BRR6-4; Sequence=VSP_013550, VSP_013551, VSP_013552;
CC Name=5;
CC IsoId=Q9BRR6-5; Sequence=VSP_013548, VSP_013553, VSP_013554;
CC Name=6;
CC IsoId=Q9BRR6-6; Sequence=VSP_035014;
CC -!- SIMILARITY: Belongs to the ADP-dependent glucokinase family.
CC {ECO:0000305}.
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DR EMBL; AF204270; AAL31473.1; -; mRNA.
DR EMBL; AL136873; CAB66807.1; -; mRNA.
DR EMBL; AK055526; BAB70941.1; -; mRNA.
DR EMBL; CR533447; CAG38478.1; -; mRNA.
DR EMBL; AK075560; BAC11699.1; -; mRNA.
DR EMBL; BC006112; AAH06112.1; -; mRNA.
DR EMBL; BC018074; AAH18074.1; -; mRNA.
DR CCDS; CCDS42057.1; -. [Q9BRR6-2]
DR RefSeq; NP_112574.3; NM_031284.4. [Q9BRR6-2]
DR RefSeq; XP_006720775.1; XM_006720712.3.
DR AlphaFoldDB; Q9BRR6; -.
DR SMR; Q9BRR6; -.
DR BioGRID; 123643; 90.
DR IntAct; Q9BRR6; 33.
DR MINT; Q9BRR6; -.
DR STRING; 9606.ENSP00000312250; -.
DR iPTMnet; Q9BRR6; -.
DR MetOSite; Q9BRR6; -.
DR PhosphoSitePlus; Q9BRR6; -.
DR SwissPalm; Q9BRR6; -.
DR BioMuta; ADPGK; -.
DR DMDM; 62899887; -.
DR EPD; Q9BRR6; -.
DR jPOST; Q9BRR6; -.
DR MassIVE; Q9BRR6; -.
DR MaxQB; Q9BRR6; -.
DR PaxDb; Q9BRR6; -.
DR PeptideAtlas; Q9BRR6; -.
DR PRIDE; Q9BRR6; -.
DR ProteomicsDB; 78812; -. [Q9BRR6-1]
DR ProteomicsDB; 78813; -. [Q9BRR6-2]
DR ProteomicsDB; 78814; -. [Q9BRR6-3]
DR ProteomicsDB; 78815; -. [Q9BRR6-4]
DR ProteomicsDB; 78816; -. [Q9BRR6-5]
DR ProteomicsDB; 78817; -. [Q9BRR6-6]
DR Antibodypedia; 26747; 218 antibodies from 30 providers.
DR DNASU; 83440; -.
DR Ensembl; ENST00000311669.12; ENSP00000312250.8; ENSG00000159322.18. [Q9BRR6-2]
DR Ensembl; ENST00000456471.3; ENSP00000397694.3; ENSG00000159322.18. [Q9BRR6-1]
DR Ensembl; ENST00000562823.1; ENSP00000454367.1; ENSG00000159322.18. [Q9BRR6-6]
DR Ensembl; ENST00000567941.5; ENSP00000458102.1; ENSG00000159322.18. [Q9BRR6-6]
DR Ensembl; ENST00000569517.5; ENSP00000454304.1; ENSG00000159322.18. [Q9BRR6-6]
DR GeneID; 83440; -.
DR KEGG; hsa:83440; -.
DR MANE-Select; ENST00000456471.3; ENSP00000397694.3; NM_001365225.1; NP_001352154.1.
DR UCSC; uc002avf.5; human. [Q9BRR6-1]
DR CTD; 83440; -.
DR GeneCards; ADPGK; -.
DR HGNC; HGNC:25250; ADPGK.
DR HPA; ENSG00000159322; Low tissue specificity.
DR MIM; 611861; gene.
DR neXtProt; NX_Q9BRR6; -.
DR OpenTargets; ENSG00000159322; -.
DR PharmGKB; PA134971928; -.
DR VEuPathDB; HostDB:ENSG00000159322; -.
DR eggNOG; KOG4184; Eukaryota.
DR GeneTree; ENSGT00390000017953; -.
DR HOGENOM; CLU_032362_0_0_1; -.
DR InParanoid; Q9BRR6; -.
DR OMA; MWRKASA; -.
DR OrthoDB; 1036373at2759; -.
DR PhylomeDB; Q9BRR6; -.
DR TreeFam; TF313401; -.
DR BRENDA; 2.7.1.147; 2681.
DR PathwayCommons; Q9BRR6; -.
DR Reactome; R-HSA-70171; Glycolysis.
DR SignaLink; Q9BRR6; -.
DR UniPathway; UPA00109; -.
DR BioGRID-ORCS; 83440; 15 hits in 1071 CRISPR screens.
DR ChiTaRS; ADPGK; human.
DR GeneWiki; ADP-specific_glucokinase; -.
DR GenomeRNAi; 83440; -.
DR Pharos; Q9BRR6; Tbio.
DR PRO; PR:Q9BRR6; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q9BRR6; protein.
DR Bgee; ENSG00000159322; Expressed in oocyte and 175 other tissues.
DR ExpressionAtlas; Q9BRR6; baseline and differential.
DR Genevisible; Q9BRR6; HS.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:CACAO.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0043843; F:ADP-specific glucokinase activity; IDA:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR GO; GO:0006006; P:glucose metabolic process; IDA:MGI.
DR GO; GO:0061620; P:glycolytic process through glucose-6-phosphate; TAS:Reactome.
DR Gene3D; 3.40.1190.20; -; 1.
DR InterPro; IPR007666; ADP_PFK/GK.
DR InterPro; IPR029056; Ribokinase-like.
DR PANTHER; PTHR21208; PTHR21208; 1.
DR Pfam; PF04587; ADP_PFK_GK; 1.
DR SUPFAM; SSF53613; SSF53613; 1.
DR PROSITE; PS51255; ADPK; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycolysis; Kinase; Magnesium; Metal-binding;
KW Reference proteome; Secreted; Signal; Transferase.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..497
FT /note="ADP-dependent glucokinase"
FT /id="PRO_0000184776"
FT DOMAIN 52..497
FT /note="ADPK"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT ACT_SITE 481
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT BINDING 297
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT BINDING 328
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT BINDING 481
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT VAR_SEQ 1..274
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:11230166, ECO:0000303|Ref.4"
FT /id="VSP_013550"
FT VAR_SEQ 1..239
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_013549"
FT VAR_SEQ 1..122
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_013548"
FT VAR_SEQ 79..497
FT /note="Missing (in isoform 6)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_035014"
FT VAR_SEQ 275..280
FT /note="RKRLLE -> MAASCR (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:11230166, ECO:0000303|Ref.4"
FT /id="VSP_013551"
FT VAR_SEQ 313
FT /note="Missing (in isoform 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:11230166,
FT ECO:0000303|PubMed:16303743, ECO:0000303|Ref.4"
FT /id="VSP_013552"
FT VAR_SEQ 404..469
FT /note="AAGARVAGTQACATETIDTSRVSLRAPQEFMTSHSEAGSRIVLNPNKPVVEW
FT HREGISFHFTPVLV -> LGSSCGWDTGLRHRNHRHQPSVSEGTPRVHDFPFGGRLQDC
FT IKPKQASSRMAQRGNILPLHTSIGV (in isoform 5)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_013553"
FT VAR_SEQ 470..497
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_013554"
FT VARIANT 184
FT /note="K -> R (in dbSNP:rs8024644)"
FT /id="VAR_060085"
SQ SEQUENCE 497 AA; 54089 MW; B758E977CDA88F8F CRC64;
MALWRGSAYA GFLALAVGCV FLLEPELPGS ALRSLWSSLC LGPAPAPPGP VSPEGRLAAA
WDALIVRPVR RWRRVAVGVN ACVDVVLSGV KLLQALGLSP GNGKDHSILH SRNDLEEAFI
HFMGKGAAAE RFFSDKETFH DIAQVASEFP GAQHYVGGNA ALIGQKFAAN SDLKVLLCGP
VGPKLHELLD DNVFVPPESL QEVDEFHLIL EYQAGEEWGQ LKAPHANRFI FSHDLSNGAM
NMLEVFVSSL EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTSISDIPT GIPVHLELAS
MTNRELMSSI VHQQVFPAVT SLGLNEQELL FLTQSASGPH SSLSSWNGVP DVGMVSDILF
WILKEHGRSK SRASDLTRIH FHTLVYHILA TVDGHWANQL AAVAAGARVA GTQACATETI
DTSRVSLRAP QEFMTSHSEA GSRIVLNPNK PVVEWHREGI SFHFTPVLVC KDPIRTVGLG
DAISAEGLFY SEVHPHY
