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ADPGK_MOUSE
ID   ADPGK_MOUSE             Reviewed;         496 AA.
AC   Q8VDL4; Q8C7Y9; Q9D0H2;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 2.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=ADP-dependent glucokinase;
DE            Short=ADP-GK;
DE            Short=ADPGK;
DE            EC=2.7.1.147;
DE   Flags: Precursor;
GN   Name=Adpgk;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   STRAIN=C57BL/6J; TISSUE=Cerebellum, and Vagina;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   CHARACTERIZATION, SUBUNIT, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=14975750; DOI=10.1016/j.bbrc.2004.01.103;
RA   Ronimus R.S., Morgan H.W.;
RT   "Cloning and biochemical characterization of a novel mouse ADP-dependent
RT   glucokinase.";
RL   Biochem. Biophys. Res. Commun. 315:652-658(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC   Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Catalyzes the phosphorylation of D-glucose to D-glucose 6-
CC       phosphate using ADP as the phosphate donor. GDP and CDP can replace
CC       ADP, but with reduced efficiency.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ADP + D-glucose = AMP + D-glucose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:11460, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:61548, ChEBI:CHEBI:456215, ChEBI:CHEBI:456216;
CC         EC=2.7.1.147;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00584};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|PROSITE-
CC       ProRule:PRU00584};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=96 uM for D-glucose (at 37 degrees Celsius)
CC         {ECO:0000269|PubMed:14975750};
CC         KM=0.28 mM for ADP (at 37 degrees Celsius)
CC         {ECO:0000269|PubMed:14975750};
CC         KM=20 mM for ADP (at 37 degrees Celsius)
CC         {ECO:0000269|PubMed:14975750};
CC         Vmax=160 umol/min/mg enzyme with glucose and ADP as substrates (at 37
CC         degrees Celsius) {ECO:0000269|PubMed:14975750};
CC       pH dependence:
CC         Optimum pH is 5.75-6.5 and 8.75-9.0. {ECO:0000269|PubMed:14975750};
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis. {ECO:0000255|PROSITE-
CC       ProRule:PRU00584}.
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:14975750}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q8VDL4-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8VDL4-2; Sequence=VSP_013555, VSP_013556;
CC       Name=3;
CC         IsoId=Q8VDL4-3; Sequence=VSP_013557;
CC   -!- SIMILARITY: Belongs to the ADP-dependent glucokinase family.
CC       {ECO:0000305}.
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DR   EMBL; AK011434; BAB27619.1; -; mRNA.
DR   EMBL; AK048904; BAC33486.1; -; mRNA.
DR   EMBL; BC021526; AAH21526.1; -; mRNA.
DR   CCDS; CCDS23248.1; -. [Q8VDL4-1]
DR   CCDS; CCDS90583.1; -. [Q8VDL4-3]
DR   RefSeq; NP_082397.1; NM_028121.2.
DR   PDB; 5CCF; X-ray; 2.10 A; A=51-496.
DR   PDB; 5CK7; X-ray; 2.99 A; A=51-496.
DR   PDBsum; 5CCF; -.
DR   PDBsum; 5CK7; -.
DR   AlphaFoldDB; Q8VDL4; -.
DR   SMR; Q8VDL4; -.
DR   BioGRID; 215179; 4.
DR   STRING; 10090.ENSMUSP00000026266; -.
DR   PhosphoSitePlus; Q8VDL4; -.
DR   SwissPalm; Q8VDL4; -.
DR   EPD; Q8VDL4; -.
DR   jPOST; Q8VDL4; -.
DR   MaxQB; Q8VDL4; -.
DR   PaxDb; Q8VDL4; -.
DR   PeptideAtlas; Q8VDL4; -.
DR   PRIDE; Q8VDL4; -.
DR   ProteomicsDB; 296113; -. [Q8VDL4-1]
DR   ProteomicsDB; 296114; -. [Q8VDL4-2]
DR   ProteomicsDB; 296115; -. [Q8VDL4-3]
DR   DNASU; 72141; -.
DR   GeneID; 72141; -.
DR   KEGG; mmu:72141; -.
DR   UCSC; uc009pxo.1; mouse. [Q8VDL4-2]
DR   CTD; 83440; -.
DR   MGI; MGI:1919391; Adpgk.
DR   eggNOG; KOG4184; Eukaryota.
DR   InParanoid; Q8VDL4; -.
DR   OrthoDB; 1036373at2759; -.
DR   PhylomeDB; Q8VDL4; -.
DR   TreeFam; TF313401; -.
DR   BRENDA; 2.7.1.147; 3474.
DR   Reactome; R-MMU-70171; Glycolysis.
DR   SABIO-RK; Q8VDL4; -.
DR   UniPathway; UPA00109; -.
DR   BioGRID-ORCS; 72141; 2 hits in 72 CRISPR screens.
DR   ChiTaRS; Adpgk; mouse.
DR   PRO; PR:Q8VDL4; -.
DR   Proteomes; UP000000589; Unplaced.
DR   RNAct; Q8VDL4; protein.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0043843; F:ADP-specific glucokinase activity; IDA:MGI.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR   GO; GO:0006006; P:glucose metabolic process; IDA:MGI.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.1190.20; -; 1.
DR   InterPro; IPR007666; ADP_PFK/GK.
DR   InterPro; IPR029056; Ribokinase-like.
DR   PANTHER; PTHR21208; PTHR21208; 1.
DR   Pfam; PF04587; ADP_PFK_GK; 1.
DR   SUPFAM; SSF53613; SSF53613; 1.
DR   PROSITE; PS51255; ADPK; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Glycolysis; Kinase; Magnesium;
KW   Metal-binding; Reference proteome; Secreted; Signal; Transferase.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..496
FT                   /note="ADP-dependent glucokinase"
FT                   /id="PRO_0000184777"
FT   DOMAIN          52..496
FT                   /note="ADPK"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT   ACT_SITE        481
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT   BINDING         297
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT   BINDING         328
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT   BINDING         481
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00584"
FT   VAR_SEQ         154..159
FT                   /note="HYVGGN -> VCPTHQ (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_013555"
FT   VAR_SEQ         160..496
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_013556"
FT   VAR_SEQ         313
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_013557"
FT   CONFLICT        318
FT                   /note="A -> T (in Ref. 1; BAB27619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        341
FT                   /note="S -> A (in Ref. 1; BAB27619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        373
FT                   /note="A -> S (in Ref. 2; AAH21526)"
FT                   /evidence="ECO:0000305"
FT   HELIX           53..64
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          73..79
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          82..88
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           89..95
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          107..109
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           112..125
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          129..133
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           136..148
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          153..158
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           159..168
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          173..177
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           183..188
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           197..199
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          206..212
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          227..232
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           235..238
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   TURN            239..242
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           243..253
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          256..260
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           263..266
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           271..287
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          294..297
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           304..313
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           316..318
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          320..324
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           326..335
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   TURN            339..342
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           352..366
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   TURN            370..372
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          378..382
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          384..392
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          395..397
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           399..415
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          424..427
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          431..435
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   TURN            437..440
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          443..445
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          453..456
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          458..466
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   STRAND          468..472
FT                   /evidence="ECO:0007829|PDB:5CCF"
FT   HELIX           479..489
FT                   /evidence="ECO:0007829|PDB:5CCF"
SQ   SEQUENCE   496 AA;  53902 MW;  CAFCB41D50131A54 CRC64;
     MALWRGSACA GFLALAVGCV FLLEPELPGT ALRSLWSSLR LGPAPVPVGP LSPESRLAAA
     WDALIAQPAR RWRRVAVGVN ACVDVVISGV KLLQALGLSP GSGKDHAILH SRSDLEEAFL
     YFMGKGAAAE RFFSDKETFH DIAQAASEFP GAQHYVGGNA ALIGQRFAAN TDLKVLLCGP
     IGPKLHELLD DNVFVPPESL QEEDEFHLIL EYLAGEEWGP FKAPHANRFI FSHDLSNGAM
     NMLEVFVSSL EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTAISDIPT GIPVHLELAS
     MTNRELMSSI VHQQVFPAVA SLGLNEQELL FLSQSASGPH SSLSSWDGVP DVGMVSDILF
     WILKEHGRSE NRASDLTRIH FHTLVYHILA TVDGHWANQL AAVAAGARVA GTQACATETI
     DTNRVSLRAP QEFTTSHLES GSRIVLNPDK PVVEWHREGI TFHFTPVLVC KDPVRTVGLG
     DAISAEGLFY SEARPD
 
 
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