EAT17_CAEEL
ID EAT17_CAEEL Reviewed; 888 AA.
AC S6F527; Q19101; S6EZL6; S6FMY8; S6FWN8; U4PBB2;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Rab GTPase-activating protein eat-17 {ECO:0000303|PubMed:23792950};
DE Short=RabGAP eat-17 {ECO:0000303|PubMed:23792950};
DE AltName: Full=Abnormal pharyngeal pumping eat-17 {ECO:0000305};
GN Name=eat-17 {ECO:0000303|PubMed:23792950, ECO:0000312|WormBase:T24D11.1e};
GN Synonyms=tbc-4 {ECO:0000312|WormBase:T24D11.1e};
GN ORFNames=T24D11.1 {ECO:0000312|WormBase:T24D11.1e};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, INTERACTION WITH RAB-6.2, TISSUE SPECIFICITY, AND MUTAGENESIS OF
RP ARG-179 AND 266-GLN--HIS-888.
RX PubMed=23792950; DOI=10.1534/genetics.113.152538;
RA Straud S., Lee I., Song B., Avery L., You Y.J.;
RT "The jaw of the worm: GTPase-activating protein EAT-17 regulates grinder
RT formation in Caenorhabditis elegans.";
RL Genetics 195:115-125(2013).
CC -!- FUNCTION: Rab GTPase activating protein for the small GTPase rab-6.2
CC (PubMed:23792950). Required for grinder formation, which is the feeding
CC organ that breaks down food (PubMed:23792950).
CC {ECO:0000269|PubMed:23792950, ECO:0000303|PubMed:23792950}.
CC -!- SUBUNIT: May interact with rab-6.2 (in GTP-bound form).
CC {ECO:0000269|PubMed:23792950}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Name=e {ECO:0000312|WormBase:T24D11.1e};
CC IsoId=S6F527-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:T24D11.1a};
CC IsoId=S6F527-2; Sequence=VSP_061178, VSP_061179;
CC Name=b {ECO:0000312|WormBase:T24D11.1b};
CC IsoId=S6F527-3; Sequence=VSP_061179;
CC Name=c {ECO:0000312|WormBase:T24D11.1c};
CC IsoId=S6F527-4; Sequence=VSP_061176;
CC Name=d {ECO:0000312|WormBase:T24D11.1d};
CC IsoId=S6F527-5; Sequence=VSP_061177, VSP_061179;
CC Name=f {ECO:0000312|WormBase:T24D11.1f};
CC IsoId=S6F527-6; Sequence=VSP_061175;
CC -!- TISSUE SPECIFICITY: Highly expressed in the terminal bulb muscles,
CC pharyngeal muscle, in intestine and vulva.
CC {ECO:0000269|PubMed:23792950}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX284606; CCD68908.1; -; Genomic_DNA.
DR EMBL; BX284606; CDG24093.1; -; Genomic_DNA.
DR EMBL; BX284606; CDG24094.1; -; Genomic_DNA.
DR EMBL; BX284606; CDG24095.1; -; Genomic_DNA.
DR EMBL; BX284606; CDG24096.1; -; Genomic_DNA.
DR EMBL; BX284606; CDH93027.1; -; Genomic_DNA.
DR PIR; T29353; T29353.
DR RefSeq; NP_001294784.1; NM_001307855.1.
DR AlphaFoldDB; S6F527; -.
DR SMR; S6F527; -.
DR DIP; DIP-27429N; -.
DR IntAct; S6F527; 3.
DR STRING; 6239.T24D11.1e; -.
DR EPD; S6F527; -.
DR EnsemblMetazoa; T24D11.1a.1; T24D11.1a.1; WBGene00020770. [S6F527-2]
DR EnsemblMetazoa; T24D11.1b.1; T24D11.1b.1; WBGene00020770. [S6F527-3]
DR EnsemblMetazoa; T24D11.1c.1; T24D11.1c.1; WBGene00020770. [S6F527-4]
DR EnsemblMetazoa; T24D11.1d.1; T24D11.1d.1; WBGene00020770. [S6F527-5]
DR EnsemblMetazoa; T24D11.1e.1; T24D11.1e.1; WBGene00020770. [S6F527-1]
DR EnsemblMetazoa; T24D11.1f.1; T24D11.1f.1; WBGene00020770. [S6F527-6]
DR GeneID; 3565805; -.
DR UCSC; F01G12.6; c. elegans.
DR CTD; 3565805; -.
DR WormBase; T24D11.1a; CE48459; WBGene00020770; eat-17. [S6F527-2]
DR WormBase; T24D11.1b; CE48495; WBGene00020770; eat-17. [S6F527-3]
DR WormBase; T24D11.1c; CE07015; WBGene00020770; eat-17. [S6F527-4]
DR WormBase; T24D11.1d; CE48465; WBGene00020770; eat-17. [S6F527-5]
DR WormBase; T24D11.1e; CE48484; WBGene00020770; eat-17. [S6F527-1]
DR WormBase; T24D11.1f; CE48533; WBGene00020770; eat-17. [S6F527-6]
DR eggNOG; KOG4436; Eukaryota.
DR GeneTree; ENSGT00940000169844; -.
DR HOGENOM; CLU_553888_0_0_1; -.
DR OMA; GHIVADW; -.
DR OrthoDB; 753960at2759; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00020770; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR ExpressionAtlas; S6F527; baseline and differential.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR GO; GO:2000253; P:positive regulation of feeding behavior; IMP:UniProtKB.
DR InterPro; IPR031214; Evi5.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR PANTHER; PTHR22957:SF254; PTHR22957:SF254; 1.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; GTPase activation; Reference proteome.
FT CHAIN 1..888
FT /note="Rab GTPase-activating protein eat-17"
FT /id="PRO_0000453575"
FT DOMAIN 173..357
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT REGION 41..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 104..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 631..654
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 854..888
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 694..770
FT /evidence="ECO:0000255"
FT COMPBIAS 41..60
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 631..653
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 860..874
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..492
FT /note="Missing (in isoform f)"
FT /evidence="ECO:0000305"
FT /id="VSP_061175"
FT VAR_SEQ 1..439
FT /note="MVPSPALVGTPSVFPHHQSTSFFQETASFFNLDWERLWHSRSNSNSTSSPRN
FT SPSQLSPPMAATAALRRSSDYGSADTECGEPCETGAPVSLNEVDLLAKMEQLNKSNEED
FT SRSVASKKTGSSESRKGAREHSPEEDEYFQEDLWSVWGELILNWEIEVKKRPNYIKDLV
FT KRGIPQHFRMIAWQNLSNASVSSVHDLYSDYMRQSSVYEKVIQRDIPRTYPELDFFKDG
FT ERGQSLLFNVIKAYSVHDKEVGYCQGSAFIVGLLLLQMPEEEAFAVLVSLMENYRLREL
FT YKPTMTDLGLCMFQLECLVQDQMPDLYTHFNNMGFDTSMYASSWFLTLFTTTMPLDIAN
FT RIMDCFLVEGMDFIFCISIAILQQARIELLRLDMEGMLKYFQREVRERYEFDADLLFTV
FT ANQVQLNAKRMKRLEKDYLTKRTKEQEEAVELR -> MRTRAQIGKQRFVWTKK (in
FT isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_061176"
FT VAR_SEQ 1..100
FT /note="Missing (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_061177"
FT VAR_SEQ 1..60
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_061178"
FT VAR_SEQ 139..141
FT /note="Missing (in isoform a, isoform b and isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_061179"
FT MUTAGEN 179
FT /note="R->A: Grinder formation defects are rescued in 1.6%
FT of animals in an eat-17 ad707 mutant background."
FT /evidence="ECO:0000269|PubMed:23792950"
FT MUTAGEN 179
FT /note="R->K: Grinder formation defects are rescued in 4.1%
FT of animals in an eat-17 ad707 mutant background."
FT /evidence="ECO:0000269|PubMed:23792950"
FT MUTAGEN 266..888
FT /note="Missing: In ad707; defective grinder formation
FT characterized by disorganized cuticle ridges in the grinder
FT and the absence of grinder plates resulting in ineffective
FT grinding of bacteria."
FT /evidence="ECO:0000269|PubMed:23792950"
SQ SEQUENCE 888 AA; 101803 MW; C40B1D083A89B546 CRC64;
MVPSPALVGT PSVFPHHQST SFFQETASFF NLDWERLWHS RSNSNSTSSP RNSPSQLSPP
MAATAALRRS SDYGSADTEC GEPCETGAPV SLNEVDLLAK MEQLNKSNEE DSRSVASKKT
GSSESRKGAR EHSPEEDEYF QEDLWSVWGE LILNWEIEVK KRPNYIKDLV KRGIPQHFRM
IAWQNLSNAS VSSVHDLYSD YMRQSSVYEK VIQRDIPRTY PELDFFKDGE RGQSLLFNVI
KAYSVHDKEV GYCQGSAFIV GLLLLQMPEE EAFAVLVSLM ENYRLRELYK PTMTDLGLCM
FQLECLVQDQ MPDLYTHFNN MGFDTSMYAS SWFLTLFTTT MPLDIANRIM DCFLVEGMDF
IFCISIAILQ QARIELLRLD MEGMLKYFQR EVRERYEFDA DLLFTVANQV QLNAKRMKRL
EKDYLTKRTK EQEEAVELRR LRTENRLLRQ RIDYLEAESS ALADRLVKGQ VNLAQEAENY
INIAHELNKL RDMNSDVHRK LEGAYETIRE LSSARRDNIM DTGTQVDDTS MIEHIHSLQQ
ELIEAHTRQA DSENTLRDAK LRVSELEMAN KRLLENEPSE DVAGLQEELI SVKMREAESS
LALKEMRQRL AELEQHWAKY VHVRAFDPSS ASIEKESTSE AHSTQQQPSP PLTSARARLA
KITASLIGGS TEETDNCISV RELEDQLMGV RIKEADTLAE LKEMRQKVME LETQNHVCTN
QLKRQDEEMK RVREDSEVLV KKRKELEDQL KDEKEKLDNK ESEFNEGRIN DRLKYSEAMQ
TIQDLQSSIS QLELKKAEKW TQNQLRGSSV CDLDEESNSH GSICSNVDHL SLASDEMNAL
LADMTVRIPT LDDLAEEGSA TETDELRPKE LNDGNDTTDS GVQLSDSH
