EAT1_ORYSJ
ID EAT1_ORYSJ Reviewed; 464 AA.
AC Q7X8R0; Q7FAG1;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Transcription factor EAT1 {ECO:0000305};
DE AltName: Full=Basic helix-loop-helix protein 141 {ECO:0000305};
DE Short=OsbHLH141 {ECO:0000303|PubMed:16896230};
DE AltName: Full=Protein DELAYED TAPETUM DEGENERATION {ECO:0000303|PubMed:23519457};
DE AltName: Full=Protein ETERNAL TAPETUM 1 {ECO:0000303|PubMed:23385589};
GN Name=EAT1 {ECO:0000303|PubMed:23385589};
GN Synonyms=BHLH141 {ECO:0000305}, DTD {ECO:0000303|PubMed:23519457};
GN OrderedLocusNames=Os04g0599300 {ECO:0000312|EMBL:BAF15663.1},
GN LOC_Os04g51070 {ECO:0000305};
GN ORFNames=OsJ_16023 {ECO:0000312|EMBL:EEE61606.1},
GN OSJNBa0083N12.3 {ECO:0000312|EMBL:CAE03466.2},
GN OSJNba0093F12.24 {ECO:0000312|EMBL:CAE03950.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16896230; DOI=10.1104/pp.106.080580;
RA Li X., Duan X., Jiang H., Sun Y., Tang Y., Yuan Z., Guo J., Liang W.,
RA Chen L., Yin J., Ma H., Wang J., Zhang D.;
RT "Genome-wide analysis of basic/helix-loop-helix transcription factor family
RT in rice and Arabidopsis.";
RL Plant Physiol. 141:1167-1184(2006).
RN [7]
RP FUNCTION, INTERACTION WITH TDR, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=23519457; DOI=10.1093/mp/sst046;
RA Ji C., Li H., Chen L., Xie M., Wang F., Chen Y., Liu Y.G.;
RT "A novel rice bHLH transcription factor, DTD, acts coordinately with TDR in
RT controlling tapetum function and pollen development.";
RL Mol. Plant 6:1715-1718(2013).
RN [8]
RP FUNCTION, INTERACTION WITH TDR, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=23385589; DOI=10.1038/ncomms2396;
RA Niu N., Liang W., Yang X., Jin W., Wilson Z.A., Hu J., Zhang D.;
RT "EAT1 promotes tapetal cell death by regulating aspartic proteases during
RT male reproductive development in rice.";
RL Nat. Commun. 4:1445-1445(2013).
CC -!- FUNCTION: Transcription factor involved in the regulation of tapetum
CC programmed cell death (PCD) and degradation during male reproductive
CC development (PubMed:23519457, PubMed:23385589). Interacts with TDR and
CC promote tapetal PCD by regulating the expression of RTS, and the two
CC lipid-transfer proteins C4 and C6, which function in microspore
CC development (PubMed:23519457). Acts downstream from and interacts with
CC TDR in the regulation of tapetal PCD. Regulates directly the aspartic
CC protease AP25 and AP37 during tapetal PCD (PubMed:23385589). May not
CC target the cysteine protease CP1 (PubMed:23519457, PubMed:23385589).
CC {ECO:0000269|PubMed:23385589, ECO:0000269|PubMed:23519457}.
CC -!- SUBUNIT: Interacts with TDR. {ECO:0000269|PubMed:23385589,
CC ECO:0000269|PubMed:23519457}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:23385589, ECO:0000269|PubMed:23519457}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing anthers from stage 8 to
CC stage 10 (PubMed:23519457). Expressed in developing anthers from stage
CC 7 to stage 11 with a peak at stages 8 and 9. Highly expressed in the
CC tapetum (PubMed:23385589). {ECO:0000269|PubMed:23385589,
CC ECO:0000269|PubMed:23519457}.
CC -!- DISRUPTION PHENOTYPE: Male sterility due to failure of tapetum
CC programmed cell death (PCD) and degeneration required for pollen
CC maturation (PubMed:23519457). Male sterility due to delayed tapetal PCD
CC and aborted pollen development (PubMed:23385589).
CC {ECO:0000269|PubMed:23385589, ECO:0000269|PubMed:23519457}.
CC -!- SIMILARITY: Belongs to the bHLH protein family.
CC -!- CAUTION: Contains a degenerate basic motif not likely to bind DNA.
CC {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAE03950.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL606683; CAE03466.2; -; Genomic_DNA.
DR EMBL; AL607004; CAE03950.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008210; BAF15663.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS90820.1; -; Genomic_DNA.
DR EMBL; CM000141; EEE61606.1; -; Genomic_DNA.
DR RefSeq; XP_015634639.1; XM_015779153.1.
DR RefSeq; XP_015634640.1; XM_015779154.1.
DR RefSeq; XP_015634641.1; XM_015779155.1.
DR AlphaFoldDB; Q7X8R0; -.
DR SMR; Q7X8R0; -.
DR STRING; 4530.OS04T0599300-01; -.
DR PaxDb; Q7X8R0; -.
DR PRIDE; Q7X8R0; -.
DR EnsemblPlants; Os04t0599300-01; Os04t0599300-01; Os04g0599300.
DR GeneID; 4336865; -.
DR Gramene; Os04t0599300-01; Os04t0599300-01; Os04g0599300.
DR KEGG; osa:4336865; -.
DR eggNOG; ENOG502QQIQ; Eukaryota.
DR HOGENOM; CLU_045325_0_0_1; -.
DR InParanoid; Q7X8R0; -.
DR OMA; MDDRQFE; -.
DR OrthoDB; 948814at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000007752; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0048658; P:anther wall tapetum development; IMP:UniProtKB.
DR GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR GO; GO:0043068; P:positive regulation of programmed cell death; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd18918; bHLH_AtMYC1_like; 1.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR045895; bHLH91-like.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR045896; MYC1-like_bHLH.
DR PANTHER; PTHR46834; PTHR46834; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..464
FT /note="Transcription factor EAT1"
FT /id="PRO_0000436456"
FT DOMAIN 261..310
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 261..274
FT /note="Basic motif; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 275..310
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 338..357
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 464 AA; 51522 MW; 7C657E0A53A431C3 CRC64;
MIVGAGYFED SHDQSLMAGS LIHDSNQAPA SSENTSIDLQ KFKVHPYSTE ALSNTANLAE
AARAINHLQH QLEIDLEQEV PPVETANWDP AICTIPDHII NHQFSEDPQN ILVEQQIQQY
DSALYPNGVY TPAPDLLNLM QCTMAPAFPA TTSVFGDTTL NGTNYLDLNG ELTGVAAVPD
SGSGLMFASD SALQLGYHGT QSHLIKDICH SLPQNYGLFP SEDERDVIIG VGSGDLFQEI
DDRQFDSVLE CRRGKGEFGK GKGKANFATE RERREQLNVK FRTLRMLFPN PTKNDRASIV
GDAIEYIDEL NRTVKELKIL VEQKRHGNNR RKVLKLDQEA AADGESSSMR PVRDDQDNQL
HGAIRSSWVQ RRSKECHVDV RIVDDEVNIK LTEKKKANSL LHAAKVLDEF QLELIHVVGG
IIGDHHIFMF NTKVSEGSAV YACAVAKKLL QAVDVQHQAL DIFN
