ID   EAT2_CYBFA              Reviewed;         371 AA.
AC   A0A061AYY2;
DT   16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT   03-SEP-2014, sequence version 1.
DT   03-AUG-2022, entry version 26.
DE   RecName: Full=Probable alcohol acetyltransferase;
DE            Short=AAT;
DE            EC=2.3.1.-;
GN   Name=EAT2; ORFNames=BON22_2183, CYFA0S_05e02014g;
OS   Cyberlindnera fabianii (Yeast) (Hansenula fabianii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Cyberlindnera.
OX   NCBI_TaxID=36022;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJS4271;
RX   PubMed=25103752; DOI=10.1128/genomea.00638-14;
RA   Freel K.C., Sarilar V., Neuveglise C., Devillers H., Friedrich A.,
RA   Schacherer J.;
RT   "Genome sequence of the yeast Cyberlindnera fabianii (Hansenula
RT   fabianii).";
RL   Genome Announc. 2:E00638-E00638(2014).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=65;
RX   PubMed=28385833; DOI=10.1128/genomea.00064-17;
RA   van Rijswijck I.M.H., Derks M.F.L., Abee T., de Ridder D., Smid E.J.;
RT   "Genome sequences of Cyberlindnera fabianii 65, Pichia kudriavzevii 129,
RT   and Saccharomyces cerevisiae 131 isolated from fermented masau fruits in
RT   Zimbabwe.";
RL   Genome Announc. 5:E00064-E00064(2017).
RN   [3]
RP   FUNCTION.
RC   STRAIN=CBS 5640;
RX   PubMed=28356220; DOI=10.1016/j.ymben.2017.03.004;
RA   Kruis A.J., Levisson M., Mars A.E., van der Ploeg M., Garces Daza F.,
RA   Ellena V., Kengen S.W.M., van der Oost J., Weusthuis R.A.;
RT   "Ethyl acetate production by the elusive alcohol acetyltransferase from
RT   yeast.";
RL   Metab. Eng. 41:92-101(2017).
CC   -!- FUNCTION: Probable alcohol acetyltransferase that uses acetyl-CoA to
CC       synthesize acetate esters from various alcohols (Probable). Not
CC       involved in the synthesis of ethyl acetate (PubMed:28356220).
CC       {ECO:0000269|PubMed:28356220, ECO:0000305|PubMed:28356220}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
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DR   EMBL; LK052890; CDR40574.1; -; Genomic_DNA.
DR   EMBL; MPUK01000003; ONH67986.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A061AYY2; -.
DR   SMR; A0A061AYY2; -.
DR   STRING; 36022.A0A061AYY2; -.
DR   ESTHER; cybfa-a0a061ayy2; ABHD11-Acetyl_transferase.
DR   EnsemblFungi; CDR40574; CDR40574; CYFA0S_05e02014g.
DR   VEuPathDB; FungiDB:BON22_2183; -.
DR   OMA; KPYDYIT; -.
DR   OrthoDB; 1268438at2759; -.
DR   PhylomeDB; A0A061AYY2; -.
DR   Proteomes; UP000189513; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR022742; Hydrolase_4.
DR   Pfam; PF12146; Hydrolase_4; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Transferase.
FT   CHAIN           1..371
FT                   /note="Probable alcohol acetyltransferase"
FT                   /id="PRO_0000446174"
FT   REGION          325..352
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        124
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:A0A1E3P8S6"
FT   ACT_SITE        295
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:A0A1E3P8S6"
SQ   SEQUENCE   371 AA;  42144 MW;  8653AE0625192649 CRC64;
     MRSTSLLMQA KAAIPDIKQL PTKHKVSLAY TIHWPHKTVN PNINIKPREP VIFLHGVFGS
     KKNYRDYCQT IANQNYTPVY SLDFRNHGES EHAFPLVNYS TLTQDVVDFI HEHKLEKVDI
     VGYSLGAKVA LLTLLKHPEL CRSGVIIGNA PIKTPQVKVY LKAFIKALKA LGDKRPEIKS
     NDKAWRAKAR DVMRKYIPDG DILHYLLRNI DIRKPKNITE YKPGTINFSM PISHFDNKVV
     EDIADWPEEE VEGLKFEGPV RVIRGTQSVF INDKGLAAYQ KHFPNYTLTN FNSNHLIWAE
     RPLQVTKVVS DFLKKTRLLE MDGSAKALEE SPKESYSRPP AHQQPLHKND FTHIDVGALP
     VNGAQAESTQ A