ID   EAT2_CYBJN              Reviewed;         354 AA.
AC   A0A1E4S2N7; A0A0H5C9V5;
DT   16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT   18-JAN-2017, sequence version 1.
DT   25-MAY-2022, entry version 19.
DE   RecName: Full=Probable alcohol acetyltransferase;
DE            Short=AAT;
DE            EC=2.3.1.-;
GN   Name=EAT2; ORFNames=BN1211_6161, CYBJADRAFT_76798;
OS   Cyberlindnera jadinii (strain ATCC 18201 / CBS 1600 / BCRC 20928 / JCM 3617
OS   / NBRC 0987 / NRRL Y-1542) (Torula yeast) (Candida utilis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Cyberlindnera.
OX   NCBI_TaxID=983966;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 18201 / CBS 1600 / BCRC 20928 / JCM 3617 / NBRC 0987 / NRRL
RC   Y-1542;
RX   PubMed=26150016; DOI=10.1016/j.jbiotec.2015.06.423;
RA   Rupp O., Brinkrolf K., Buerth C., Kunigo M., Schneider J., Jaenicke S.,
RA   Goesmann A., Puehler A., Jaeger K.-E., Ernst J.F.;
RT   "The structure of the Cyberlindnera jadinii genome and its relation to
RT   Candida utilis analyzed by the occurrence of single nucleotide
RT   polymorphisms.";
RL   J. Biotechnol. 211:20-30(2015).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 18201 / CBS 1600 / BCRC 20928 / JCM 3617 / NBRC 0987 / NRRL
RC   Y-1542;
RX   PubMed=27535936; DOI=10.1073/pnas.1603941113;
RA   Riley R., Haridas S., Wolfe K.H., Lopes M.R., Hittinger C.T., Goeker M.,
RA   Salamov A.A., Wisecaver J.H., Long T.M., Calvey C.H., Aerts A.L.,
RA   Barry K.W., Choi C., Clum A., Coughlan A.Y., Deshpande S., Douglass A.P.,
RA   Hanson S.J., Klenk H.-P., LaButti K.M., Lapidus A., Lindquist E.A.,
RA   Lipzen A.M., Meier-Kolthoff J.P., Ohm R.A., Otillar R.P., Pangilinan J.L.,
RA   Peng Y., Rokas A., Rosa C.A., Scheuner C., Sibirny A.A., Slot J.C.,
RA   Stielow J.B., Sun H., Kurtzman C.P., Blackwell M., Grigoriev I.V.,
RA   Jeffries T.W.;
RT   "Comparative genomics of biotechnologically important yeasts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 113:9882-9887(2016).
RN   [3]
RP   FUNCTION.
RC   STRAIN=DSM 2361;
RX   PubMed=28356220; DOI=10.1016/j.ymben.2017.03.004;
RA   Kruis A.J., Levisson M., Mars A.E., van der Ploeg M., Garces Daza F.,
RA   Ellena V., Kengen S.W.M., van der Oost J., Weusthuis R.A.;
RT   "Ethyl acetate production by the elusive alcohol acetyltransferase from
RT   yeast.";
RL   Metab. Eng. 41:92-101(2017).
CC   -!- FUNCTION: Probable alcohol acetyltransferase that uses acetyl-CoA to
CC       synthesize acetate esters from various alcohols (Probable). Not
CC       involved in the synthesis of ethyl acetate (PubMed:28356220).
CC       {ECO:0000269|PubMed:28356220, ECO:0000305|PubMed:28356220}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CDQK01000007; CEP25158.1; -; Genomic_DNA.
DR   EMBL; KV453929; ODV73797.1; -; Genomic_DNA.
DR   RefSeq; XP_020070836.1; XM_020217932.1.
DR   AlphaFoldDB; A0A1E4S2N7; -.
DR   SMR; A0A1E4S2N7; -.
DR   STRING; 983966.A0A1E4S2N7; -.
DR   ESTHER; cybja-a0a0h5c9v5; ABHD11-Acetyl_transferase.
DR   EnsemblFungi; CEP25158; CEP25158; BN1211_6161.
DR   EnsemblFungi; ODV73797; ODV73797; CYBJADRAFT_76798.
DR   GeneID; 30992328; -.
DR   OMA; KPYDYIT; -.
DR   OrthoDB; 1268438at2759; -.
DR   Proteomes; UP000038830; Unassembled WGS sequence.
DR   Proteomes; UP000094389; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR022742; Hydrolase_4.
DR   Pfam; PF12146; Hydrolase_4; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Transferase.
FT   CHAIN           1..354
FT                   /note="Probable alcohol acetyltransferase"
FT                   /id="PRO_0000446176"
FT   ACT_SITE        124
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:A0A1E3P8S6"
FT   ACT_SITE        293
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:A0A1E3P8S6"
SQ   SEQUENCE   354 AA;  39779 MW;  448F41AC7658929B CRC64;
     MQITKQLWSK AVTKAPLLPL PTKTKVSLAY KLHMPNKTVT PNLTIRSHEP IVFLHGVFGS
     KKNYGDICQT IANTTHTPVY AIDFRNHGES EHCFPLVNYS QLSKDVVDFC EEHDLKRVSI
     VGYSLGAKVG LLTMLKHPKL VNSGVIIGNA PIETPQVKVY LKVFLKAMTA LIDSAHIKAT
     DKNWREKARA VMSKYIPDAS IVQYLLRNVL NKKSSHIPGH TDLISVQMPI SHFNKDVIDD
     ISDWPAEEVK GLKFEGPVKV VKGLKSAFIT KEGEKAYAEH FPNYTMSTFN TNHLIFAEMP
     SRVTKTVCDF IKLTRYQLLQ QHTRDGKHHT IQKVDPALLN SSEASTVEQA TKQL