EAT2_WICCF
ID EAT2_WICCF Reviewed; 341 AA.
AC K0KSN3;
DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2012, sequence version 1.
DT 25-MAY-2022, entry version 33.
DE RecName: Full=Probable alcohol acetyltransferase;
DE Short=AAT;
DE EC=2.3.1.-;
GN Name=EAT2; ORFNames=BN7_4635;
OS Wickerhamomyces ciferrii (strain ATCC 14091 / BCRC 22168 / CBS 111 / JCM
OS 3599 / NBRC 0793 / NRRL Y-1031 F-60-10) (Yeast) (Pichia ciferrii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX NCBI_TaxID=1206466;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14091 / BCRC 22168 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL
RC Y-1031 F-60-10;
RX PubMed=23193139; DOI=10.1128/ec.00258-12;
RA Schneider J., Andrea H., Blom J., Jaenicke S., Rueckert C., Schorsch C.,
RA Szczepanowski R., Farwick M., Goesmann A., Puehler A., Schaffer S.,
RA Tauch A., Koehler T., Brinkrolf K.;
RT "Draft genome sequence of Wickerhamomyces ciferrii NRRL Y-1031 F-60-10.";
RL Eukaryot. Cell 11:1582-1583(2012).
RN [2]
RP FUNCTION.
RC STRAIN=ATCC 14091 / BCRC 22168 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL
RC Y-1031 F-60-10;
RX PubMed=28356220; DOI=10.1016/j.ymben.2017.03.004;
RA Kruis A.J., Levisson M., Mars A.E., van der Ploeg M., Garces Daza F.,
RA Ellena V., Kengen S.W.M., van der Oost J., Weusthuis R.A.;
RT "Ethyl acetate production by the elusive alcohol acetyltransferase from
RT yeast.";
RL Metab. Eng. 41:92-101(2017).
CC -!- FUNCTION: Probable alcohol acetyltransferase that uses acetyl-CoA to
CC synthesize acetate esters from various alcohols (Probable). Not
CC involved in the synthesis of ethyl acetate (PubMed:28356220).
CC {ECO:0000269|PubMed:28356220, ECO:0000305|PubMed:28356220}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
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DR EMBL; CAIF01000179; CCH45057.1; -; Genomic_DNA.
DR AlphaFoldDB; K0KSN3; -.
DR SMR; K0KSN3; -.
DR STRING; 1206466.K0KSN3; -.
DR ESTHER; wiccf-k0ksn3; ABHD11-Acetyl_transferase.
DR EnsemblFungi; CCH45057; CCH45057; BN7_4635.
DR eggNOG; KOG2382; Eukaryota.
DR HOGENOM; CLU_020336_53_0_1; -.
DR InParanoid; K0KSN3; -.
DR Proteomes; UP000009328; Unassembled WGS sequence.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 3: Inferred from homology;
KW Hydrolase; Reference proteome; Transferase.
FT CHAIN 1..341
FT /note="Probable alcohol acetyltransferase"
FT /id="PRO_0000446185"
FT DOMAIN 47..301
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000255"
FT ACT_SITE 120
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:A0A1E3P8S6"
FT ACT_SITE 144
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:A0A1E3P8S6"
FT ACT_SITE 294
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:A0A1E3P8S6"
SQ SEQUENCE 341 AA; 38856 MW; 4D2FA4322E2C8F11 CRC64;
MFATKILRNA QSIKNELPHR EVVKMAYDLH KPRSTAIRNL NENHEEPILF MHGIFGSKKS
YVQDSKLISS ATHTPVYTID LRNHGESAHA QPFDYATLAA DVKEFCDSHK LDKVKLVGYS
LGAKVSMLTA LQYPELVKSA VIIDNAPIPQ PQIQLFMKQY IKAMKTVLNE ANISADDKDW
KNKASAAMKR FLPNGVIRKN LLANLVNKPP KDFDSPVIDF GDGQIHFLNP IEQMEEMAVE
DVTDWPTELT KDLVFEGPVK FIRGLKSPFI TDEGYEAIQK HFPNNEFHDL NSSHDILDQR
PTEYVKIIND FFNIHRYESA PDSTILGHKD HPQTQARRSH L
