EB1_DICDI
ID EB1_DICDI Reviewed; 506 AA.
AC Q8WQ86; Q54QT3;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Microtubule-associated protein RP/EB family member 1;
DE Short=DdEB1;
GN Name=eb1; ORFNames=DDB_G0283607;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=AX2;
RX PubMed=12134070; DOI=10.1091/mbc.e02-01-0054;
RA Rehberg M., Graef R.;
RT "Dictyostelium EB1 is a genuine centrosomal component required for proper
RT spindle formation.";
RL Mol. Biol. Cell 13:2301-2310(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16782229; DOI=10.1016/j.ejcb.2006.05.008;
RA Koch K.V., Reinders Y., Ho T.-H., Sickmann A., Graef R.;
RT "Identification and isolation of Dictyostelium microtubule-associated
RT protein interactors by tandem affinity purification.";
RL Eur. J. Cell Biol. 85:1079-1090(2006).
CC -!- FUNCTION: Involved in microtubule polymerization, and spindle function
CC by stabilizing microtubules and anchoring them at centrosomes.
CC -!- SUBUNIT: Interacts with mtaA/CP224 at the microtubule tip, centrosome
CC and kinetochore. {ECO:0000269|PubMed:12134070}.
CC -!- SUBCELLULAR LOCATION: Cell projection {ECO:0000269|PubMed:12134070}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC {ECO:0000269|PubMed:12134070}. Cytoplasm {ECO:0000269|PubMed:12134070}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:12134070}.
CC Note=Associated with the microtubule network. Accumulates at the plus
CC end of microtubules.
CC -!- DISRUPTION PHENOTYPE: Defects in cytokinesis due to poor mitotic
CC spindle assembly during mitotic prometaphase.
CC {ECO:0000269|PubMed:12134070}.
CC -!- SIMILARITY: Belongs to the MAPRE family. {ECO:0000305}.
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DR EMBL; AJ426053; CAD19801.1; -; mRNA.
DR EMBL; AAFI02000056; EAL65570.1; -; Genomic_DNA.
DR RefSeq; XP_638940.1; XM_633848.1.
DR AlphaFoldDB; Q8WQ86; -.
DR SMR; Q8WQ86; -.
DR STRING; 44689.DDB0191160; -.
DR PaxDb; Q8WQ86; -.
DR PRIDE; Q8WQ86; -.
DR EnsemblProtists; EAL65570; EAL65570; DDB_G0283607.
DR GeneID; 8624179; -.
DR KEGG; ddi:DDB_G0283607; -.
DR dictyBase; DDB_G0283607; eb1.
DR eggNOG; KOG3000; Eukaryota.
DR HOGENOM; CLU_539100_0_0_1; -.
DR InParanoid; Q8WQ86; -.
DR OMA; FNAKYDY; -.
DR PRO; PR:Q8WQ86; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0000235; C:astral microtubule; IDA:dictyBase.
DR GO; GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
DR GO; GO:0031592; C:centrosomal corona; IDA:dictyBase.
DR GO; GO:0005813; C:centrosome; IDA:dictyBase.
DR GO; GO:0005881; C:cytoplasmic microtubule; IBA:GO_Central.
DR GO; GO:0000776; C:kinetochore; IDA:dictyBase.
DR GO; GO:0005874; C:microtubule; IDA:dictyBase.
DR GO; GO:0005815; C:microtubule organizing center; IBA:GO_Central.
DR GO; GO:0035371; C:microtubule plus-end; IBA:GO_Central.
DR GO; GO:0005819; C:spindle; IDA:dictyBase.
DR GO; GO:0051233; C:spindle midzone; IBA:GO_Central.
DR GO; GO:0000922; C:spindle pole; IDA:dictyBase.
DR GO; GO:0070840; F:dynein complex binding; IDA:dictyBase.
DR GO; GO:0042802; F:identical protein binding; IPI:dictyBase.
DR GO; GO:0008017; F:microtubule binding; IMP:dictyBase.
DR GO; GO:0051010; F:microtubule plus-end binding; IBA:GO_Central.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR GO; GO:0008104; P:protein localization; IMP:dictyBase.
DR GO; GO:1904825; P:protein localization to microtubule plus-end; IBA:GO_Central.
DR GO; GO:0031110; P:regulation of microtubule polymerization or depolymerization; IBA:GO_Central.
DR GO; GO:0051225; P:spindle assembly; IMP:dictyBase.
DR Gene3D; 1.10.418.10; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR004953; EB1_C.
DR InterPro; IPR036133; EB1_C_sf.
DR InterPro; IPR027328; MAPRE.
DR PANTHER; PTHR10623; PTHR10623; 1.
DR Pfam; PF00307; CH; 1.
DR Pfam; PF03271; EB1; 1.
DR SUPFAM; SSF140612; SSF140612; 1.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS50021; CH; 1.
DR PROSITE; PS51230; EB1_C; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cell projection; Centromere; Chromosome;
KW Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis;
KW Reference proteome.
FT CHAIN 1..506
FT /note="Microtubule-associated protein RP/EB family member
FT 1"
FT /id="PRO_0000328617"
FT DOMAIN 3..105
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT DOMAIN 303..371
FT /note="EB1 C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00576"
FT REGION 127..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 365..506
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 283..344
FT /evidence="ECO:0000255"
FT COILED 377..459
FT /evidence="ECO:0000255"
FT COMPBIAS 138..230
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 237..256
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 368..408
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 409..425
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..462
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 487..506
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 506 AA; 56983 MW; F20BC182BAAA639C CRC64;
MEGFGRNEIL NWINDLLQLD YKKIEQLGSG AALCQLVDII HPGKINLKMV NFNAKYDYEY
IKNFSYLQES FAKLGVEKYV EVSELVKSRP QANLEFAQWM KKYFDQYYTG EPYNAIERRI
ALKIPTDKDR SSLKGKTAAT GAPPTSKPSP SSTLKPATTA ASKPAPVSKP TTTTAKPTPS
SVSKPVSKPT PSSISKPVSK PTPSISKPVT KPTPTTTSTS TTTTVSTPPS TPKPTNTPIP
STTGKPTLTQ PTFKPTPKSV SPTPPVVAGS TVTTKTVIVS EPPTELLEEL EQQKRELEQQ
RKELEEQKSV IQEMTEKIAN FEITIQDIEK DRDFYFERLR EAEIFCQDHS DVPLLGEVLK
ILYNSNGEEE GEEGEGEEQG GEEEEEEEEQ GENNIEQEEE EQIEQQQQQQ EEEEEHERET
LSEPEPDQDN SAIPLEQLEI NDEEEEFNQH QEQEEEDEHF EDEEKVNGLN EEEIMNSVLQ
NASDEDDILE STFDGNEDDS LLQDEY
