EBF2_ARATH
ID EBF2_ARATH Reviewed; 623 AA.
AC Q708Y0;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=EIN3-binding F-box protein 2;
GN Name=EBF2; OrderedLocusNames=At5g25350; ORFNames=F18G18.90;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, INDUCTION, TISSUE
RP SPECIFICITY, SUBCELLULAR LOCATION, AND INTERACTION WITH CUL1; SKP1A/ASK1;
RP SKP1B/ASK2; EIN3 AND EIL1.
RX PubMed=14675533; DOI=10.1016/s0092-8674(03)00968-1;
RA Potuschak T., Lechner E., Parmentier Y., Yanagisawa S., Grava S., Koncz C.,
RA Genschik P.;
RT "EIN3-dependent regulation of plant ethylene hormone signaling by two
RT Arabidopsis F box proteins: EBF1 and EBF2.";
RL Cell 115:679-689(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of SCF(EBF1) E3 ubiquitin ligase complexes, which
CC may mediate the ubiquitination and subsequent proteasomal degradation
CC of target proteins (probably including EIN3 and EIL1). Regulator of the
CC ethylene signaling cascade by modulating the stability of EIN3 and EIL1
CC proteins. {ECO:0000269|PubMed:14675533}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex.
CC Interacts with CUL1, SKP1A/ASK1, SKP1B/ASK2, EIN3, and EIL1.
CC {ECO:0000269|PubMed:14675533}.
CC -!- INTERACTION:
CC Q708Y0; O24606: EIN3; NbExp=3; IntAct=EBI-593623, EBI-593576;
CC Q708Y0; P43291: SRK2A; NbExp=2; IntAct=EBI-593623, EBI-401164;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14675533}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:14675533}.
CC -!- INDUCTION: EIN3-dependent induction by ethylene.
CC {ECO:0000269|PubMed:14675533}.
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DR EMBL; AJ609239; CAE75865.1; -; Genomic_RNA.
DR EMBL; AY485830; AAR27072.1; -; mRNA.
DR EMBL; AC006258; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED93429.1; -; Genomic_DNA.
DR EMBL; AK227858; BAE99835.1; -; mRNA.
DR RefSeq; NP_197917.1; NM_122444.4.
DR AlphaFoldDB; Q708Y0; -.
DR SMR; Q708Y0; -.
DR BioGRID; 17882; 10.
DR IntAct; Q708Y0; 6.
DR STRING; 3702.AT5G25350.1; -.
DR PaxDb; Q708Y0; -.
DR PRIDE; Q708Y0; -.
DR EnsemblPlants; AT5G25350.1; AT5G25350.1; AT5G25350.
DR GeneID; 832607; -.
DR Gramene; AT5G25350.1; AT5G25350.1; AT5G25350.
DR KEGG; ath:AT5G25350; -.
DR Araport; AT5G25350; -.
DR TAIR; locus:2145512; AT5G25350.
DR eggNOG; KOG1947; Eukaryota.
DR HOGENOM; CLU_016072_2_0_1; -.
DR InParanoid; Q708Y0; -.
DR OMA; VNLSECI; -.
DR OrthoDB; 1282076at2759; -.
DR PhylomeDB; Q708Y0; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q708Y0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q708Y0; baseline and differential.
DR Genevisible; Q708Y0; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IPI:TAIR.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010105; P:negative regulation of ethylene-activated signaling pathway; TAS:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0009723; P:response to ethylene; IMP:TAIR.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:TAIR.
DR Gene3D; 3.80.10.10; -; 4.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF00646; F-box; 1.
DR Pfam; PF00560; LRR_1; 1.
DR Pfam; PF13516; LRR_6; 3.
DR SMART; SM00256; FBOX; 1.
DR SMART; SM00367; LRR_CC; 13.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Ethylene signaling pathway; Leucine-rich repeat; Nucleus;
KW Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..623
FT /note="EIN3-binding F-box protein 2"
FT /id="PRO_0000272246"
FT DOMAIN 52..106
FT /note="F-box"
FT REPEAT 119..147
FT /note="LRR 1"
FT REPEAT 151..176
FT /note="LRR 2"
FT REPEAT 177..202
FT /note="LRR 3"
FT REPEAT 203..228
FT /note="LRR 4"
FT REPEAT 229..254
FT /note="LRR 5"
FT REPEAT 255..281
FT /note="LRR 6"
FT REPEAT 307..334
FT /note="LRR 7"
FT REPEAT 335..360
FT /note="LRR 8"
FT REPEAT 361..386
FT /note="LRR 9"
FT REPEAT 387..413
FT /note="LRR 10"
FT REPEAT 414..441
FT /note="LRR 11"
FT REPEAT 442..467
FT /note="LRR 12"
FT REPEAT 468..494
FT /note="LRR 13"
FT REPEAT 495..521
FT /note="LRR 14"
FT REPEAT 522..547
FT /note="LRR 15"
FT REPEAT 548..574
FT /note="LRR 16"
FT REPEAT 575..600
FT /note="LRR 17"
FT REPEAT 601..623
FT /note="LRR 18"
SQ SEQUENCE 623 AA; 66061 MW; 990E2D9B098A11B6 CRC64;
MSGIFRFSGD EDCLLGGSMY LSPGSCPGVY YPARKRLRVA ATSFYSGFEE KQTSIDVLPE
ECLFEILRRL PSGQERSACA CVSKHWLNLL SSISRSEVNE SSVQDVEEGE GFLSRSLEGK
KATDLRLAAI AVGTSSRGGL GKLQIRGSGF ESKVTDVGLG AVAHGCPSLR IVSLWNLPAV
SDLGLSEIAR SCPMIEKLDL SRCPGITDSG LVAIAENCVN LSDLTIDSCS GVGNEGLRAI
ARRCVNLRSI SIRSCPRIGD QGVAFLLAQA GSYLTKVKLQ MLNVSGLSLA VIGHYGAAVT
DLVLHGLQGV NEKGFWVMGN AKGLKKLKSL SVMSCRGMTD VGLEAVGNGC PDLKHVSLNK
CLLVSGKGLV ALAKSALSLE SLKLEECHRI NQFGLMGFLM NCGSKLKAFS LANCLGISDF
NSESSLPSPS CSSLRSLSIR CCPGFGDASL AFLGKFCHQL QDVELCGLNG VTDAGVRELL
QSNNVGLVKV NLSECINVSD NTVSAISVCH GRTLESLNLD GCKNITNASL VAVAKNCYSV
NDLDISNTLV SDHGIKALAS SPNHLNLQVL SIGGCSSITD KSKACIQKLG RTLLGLNIQR
CGRISSSTVD TLLENLWRCD ILY
