EBFC1_ARATH
ID EBFC1_ARATH Reviewed; 180 AA.
AC Q9M098; Q8LDX3;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 131.
DE RecName: Full=Nucleoid-associated protein At4g30620, chloroplastic;
DE Flags: Precursor;
GN OrderedLocusNames=At4g30620 {ECO:0000312|EMBL:CAB79780.1};
GN ORFNames=F17I23.40 {ECO:0000312|EMBL:CAB79780.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Paz T., Hofmann K., Levanon N., Yahalom A., Chamovitz D.A.;
RT "A novel plant-specific protein interacts with both eIF3e/INT6 and COP9
RT signalosome subunits 7 and 8 from Arabidopsis.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INTERACTION WITH TIF3E1.
RX PubMed=19704582; DOI=10.4161/psb.3.6.5434;
RA Paz-Aviram T., Yahalom A., Chamovitz D.A.;
RT "Arabidopsis eIF3e interacts with subunits of the ribosome, Cop9
RT signalosome and proteasome.";
RL Plant Signal. Behav. 3:409-411(2008).
CC -!- FUNCTION: Binds to DNA and alters its conformation. May be involved in
CC regulation of gene expression, nucleoid organization and DNA
CC protection. {ECO:0000250|UniProtKB:Q9RXV7}.
CC -!- SUBUNIT: Homodimer (By similarity). Binds to the translation initiation
CC factors TIF3E1 (PubMed:19704582). {ECO:0000250|UniProtKB:P0A8B5,
CC ECO:0000269|PubMed:19704582}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the YbaB/EbfC family. {ECO:0000305}.
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DR EMBL; AY327123; AAP89016.1; -; mRNA.
DR EMBL; AL161577; CAB79780.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85787.1; -; Genomic_DNA.
DR EMBL; AY074869; AAL75890.1; -; mRNA.
DR EMBL; BT020301; AAV84522.1; -; mRNA.
DR EMBL; BT025534; ABF58952.1; -; mRNA.
DR EMBL; AY085741; AAM62959.1; -; mRNA.
DR PIR; C85358; C85358.
DR RefSeq; NP_194791.1; NM_119208.2.
DR AlphaFoldDB; Q9M098; -.
DR SMR; Q9M098; -.
DR STRING; 3702.AT4G30620.1; -.
DR iPTMnet; Q9M098; -.
DR PaxDb; Q9M098; -.
DR PRIDE; Q9M098; -.
DR ProteomicsDB; 221961; -.
DR EnsemblPlants; AT4G30620.1; AT4G30620.1; AT4G30620.
DR GeneID; 829185; -.
DR Gramene; AT4G30620.1; AT4G30620.1; AT4G30620.
DR KEGG; ath:AT4G30620; -.
DR Araport; AT4G30620; -.
DR TAIR; locus:2118721; AT4G30620.
DR eggNOG; KOG0017; Eukaryota.
DR HOGENOM; CLU_091861_0_0_1; -.
DR InParanoid; Q9M098; -.
DR OMA; PRPLQIC; -.
DR OrthoDB; 1470195at2759; -.
DR PhylomeDB; Q9M098; -.
DR PRO; PR:Q9M098; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9M098; baseline and differential.
DR Genevisible; Q9M098; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR Gene3D; 3.30.1310.10; -; 1.
DR InterPro; IPR036894; YbaB-like_sf.
DR InterPro; IPR004401; YbaB/EbfC.
DR PANTHER; PTHR33449; PTHR33449; 1.
DR Pfam; PF02575; YbaB_DNA_bd; 1.
DR SUPFAM; SSF82607; SSF82607; 1.
PE 1: Evidence at protein level;
KW Chloroplast; DNA-binding; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..48
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 49..180
FT /note="Nucleoid-associated protein At4g30620,
FT chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000434789"
FT REGION 45..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 51
FT /note="G -> V (in Ref. 1; AAP89016 and 6; AAM62959)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 180 AA; 19583 MW; BA58E98E78F34F68 CRC64;
MASTATNTDF FKTLLSPFSN GNAAQRSSRQ NIVWLNRKQS GNNNRSLRVN GLFGGGKKDN
KEDGQSKAGI LGNMQNLYET VKKAQMVVQV EAVRVQKELA VAEFDGYCQG ELVKVTLSGN
QQPIRTDITD AAMELGSEKL SLLVTEAYKD AHSKSVLAMK ERMSDLAQSL GMPPGLDGLK
