EBFC2_ARATH
ID EBFC2_ARATH Reviewed; 182 AA.
AC O82230; F4INP2; Q94AE8;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Nucleoid-associated protein At2g24020, chloroplastic {ECO:0000305};
DE AltName: Full=Suppressor of tic40 protein 2 {ECO:0000303|PubMed:28684427};
DE Flags: Precursor;
GN Name=STIC2 {ECO:0000303|PubMed:28684427};
GN OrderedLocusNames=At2g24020 {ECO:0000312|EMBL:AEC07518.1};
GN ORFNames=T29E15.22 {ECO:0000312|EMBL:AAC63670.2};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, HOMODIMERIZATION, INTERACTION WITH ALB3 AND ALB4, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=28684427; DOI=10.1105/tpc.16.00962;
RA Bedard J., Troesch R., Wu F., Ling Q., Flores-Perez U., Toepel M.,
RA Nawaz F., Jarvis P.;
RT "Suppressors of the chloroplast protein import mutant tic40 reveal a
RT genetic link between protein import and thylakoid biogenesis.";
RL Plant Cell 29:1726-1747(2017).
CC -!- FUNCTION: Participates with ALB4 in thylakoid protein targeting. May
CC function with specific subset of thylakoidal proteins
CC (PubMed:28684427). Binds to DNA and alters its conformation. May be
CC involved in regulation of gene expression, nucleoid organization and
CC DNA protection (By similarity). {ECO:0000250|UniProtKB:Q9RXV7,
CC ECO:0000269|PubMed:28684427}.
CC -!- SUBUNIT: Homodimer. Interacts with ALB3 and ALB4.
CC {ECO:0000269|PubMed:28684427}.
CC -!- INTERACTION:
CC O82230; Q8LBP4: ALB3; NbExp=4; IntAct=EBI-4428759, EBI-1806831;
CC O82230; Q9FYL3: ALB4; NbExp=4; IntAct=EBI-4428759, EBI-16630560;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000269|PubMed:28684427}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O82230-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O82230-2; Sequence=VSP_057979;
CC -!- DISRUPTION PHENOTYPE: No visible macroscopic phenotype under normal
CC growth condtions. Increased number of plastoglobules (lipid bodies) in
CC chloroplasts. {ECO:0000269|PubMed:28684427}.
CC -!- SIMILARITY: Belongs to the YbaB/EbfC family. {ECO:0000305}.
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DR EMBL; AC005170; AAC63670.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC07518.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07519.1; -; Genomic_DNA.
DR EMBL; AY048234; AAK82497.1; -; mRNA.
DR EMBL; AY091702; AAM10301.1; -; mRNA.
DR PIR; F84631; F84631.
DR RefSeq; NP_001189586.1; NM_001202657.1. [O82230-2]
DR RefSeq; NP_565561.1; NM_127964.5. [O82230-1]
DR AlphaFoldDB; O82230; -.
DR SMR; O82230; -.
DR IntAct; O82230; 14.
DR STRING; 3702.AT2G24020.1; -.
DR MetOSite; O82230; -.
DR PaxDb; O82230; -.
DR PRIDE; O82230; -.
DR ProteomicsDB; 221962; -. [O82230-1]
DR EnsemblPlants; AT2G24020.1; AT2G24020.1; AT2G24020. [O82230-1]
DR EnsemblPlants; AT2G24020.2; AT2G24020.2; AT2G24020. [O82230-2]
DR GeneID; 816936; -.
DR Gramene; AT2G24020.1; AT2G24020.1; AT2G24020. [O82230-1]
DR Gramene; AT2G24020.2; AT2G24020.2; AT2G24020. [O82230-2]
DR KEGG; ath:AT2G24020; -.
DR Araport; AT2G24020; -.
DR TAIR; locus:2061471; AT2G24020.
DR eggNOG; KOG0017; Eukaryota.
DR InParanoid; O82230; -.
DR OMA; NDHKCPS; -.
DR PhylomeDB; O82230; -.
DR PRO; PR:O82230; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82230; baseline and differential.
DR Genevisible; O82230; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0045037; P:protein import into chloroplast stroma; IGI:TAIR.
DR Gene3D; 3.30.1310.10; -; 1.
DR InterPro; IPR036894; YbaB-like_sf.
DR InterPro; IPR004401; YbaB/EbfC.
DR PANTHER; PTHR33449; PTHR33449; 1.
DR Pfam; PF02575; YbaB_DNA_bd; 1.
DR SUPFAM; SSF82607; SSF82607; 1.
DR TIGRFAMs; TIGR00103; DNA_YbaB_EbfC; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; DNA-binding; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..48
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 49..182
FT /note="Nucleoid-associated protein At2g24020,
FT chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000434790"
FT VAR_SEQ 21..22
FT /note="Missing (in isoform 2)"
FT /id="VSP_057979"
SQ SEQUENCE 182 AA; 19812 MW; 6AB58B1BB171211D CRC64;
MASMAATTNF TKSMLFPFSH VSGNASLNSQ RRTWPKQYKS KNGYRSLRVN GLFGGGNKDN
NSEDGQSKAG IFGNMQNMYE TVKKAQMVVQ VEAVRVQKEL AAAEFDGYCA GELVKVTLSG
NQQPIRTDIT EAAMELGSEK LSQLVTEAYK DAHAKSVVAM KERMSDLAQS LGMPPGLSEG
MK
