EBFC_BORBU
ID EBFC_BORBU Reviewed; 99 AA.
AC O51418;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Nucleoid-associated protein EbfC {ECO:0000255|HAMAP-Rule:MF_00274};
GN Name=ebfC {ECO:0000255|HAMAP-Rule:MF_00274}; OrderedLocusNames=BB_0462;
OS Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS (Borrelia burgdorferi).
OC Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX NCBI_TaxID=224326;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX PubMed=9403685; DOI=10.1038/37551;
RA Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA Smith H.O., Venter J.C.;
RT "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL Nature 390:580-586(1997).
RN [2]
RP FUNCTION, DNA-BINDING, SUBUNIT, AND MUTAGENESIS OF LYS-16; ASP-20; LYS-23;
RP ASN-77; ASP-78; LYS-82; LYS-84; GLU-85 AND LYS-88.
RC STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX PubMed=19208644; DOI=10.1093/nar/gkp027;
RA Riley S.P., Bykowski T., Cooley A.E., Burns L.H., Babb K., Brissette C.A.,
RA Bowman A., Rotondi M., Miller M.C., DeMoll E., Lim K., Fried M.G.,
RA Stevenson B.;
RT "Borrelia burgdorferi EbfC defines a newly-identified, widespread family of
RT bacterial DNA-binding proteins.";
RL Nucleic Acids Res. 37:1973-1983(2009).
RN [3]
RP FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX PubMed=22544270; DOI=10.1128/jb.00252-12;
RA Jutras B.L., Bowman A., Brissette C.A., Adams C.A., Verma A., Chenail A.M.,
RA Stevenson B.;
RT "EbfC (YbaB) is a new type of bacterial nucleoid-associated protein and a
RT global regulator of gene expression in the Lyme disease spirochete.";
RL J. Bacteriol. 194:3395-3406(2012).
CC -!- FUNCTION: Binds to DNA and alters its conformation. May be involved in
CC global regulation of gene expression. Binds specifically and non-
CC specifically to DNA, preferentially to the 4 bp broken palindrome 5'-
CC GTnAC-3'. Affects expression of a wide variety of genes, encoding both
CC structural and metabolic proteins. {ECO:0000269|PubMed:19208644,
CC ECO:0000269|PubMed:22544270}.
CC -!- SUBUNIT: Homodimer. Can form tetramers and octamers in solution.
CC {ECO:0000255|HAMAP-Rule:MF_00274, ECO:0000269|PubMed:19208644}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid {ECO:0000255|HAMAP-
CC Rule:MF_00274, ECO:0000269|PubMed:22544270}.
CC -!- INDUCTION: Transcribed at high levels in rapidly growing cells, but is
CC indetectable in stationary-phase cells. {ECO:0000269|PubMed:22544270}.
CC -!- SIMILARITY: Belongs to the YbaB/EbfC family. {ECO:0000255|HAMAP-
CC Rule:MF_00274}.
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DR EMBL; AE000783; AAC66830.2; -; Genomic_DNA.
DR PIR; E70157; E70157.
DR RefSeq; NP_212596.2; NC_001318.1.
DR RefSeq; WP_002557054.1; NC_001318.1.
DR AlphaFoldDB; O51418; -.
DR SMR; O51418; -.
DR STRING; 224326.BB_0462; -.
DR PRIDE; O51418; -.
DR EnsemblBacteria; AAC66830; AAC66830; BB_0462.
DR GeneID; 56567898; -.
DR KEGG; bbu:BB_0462; -.
DR PATRIC; fig|224326.49.peg.855; -.
DR HOGENOM; CLU_140930_4_1_12; -.
DR OMA; MQRIQET; -.
DR Proteomes; UP000001807; Chromosome.
DR GO; GO:0043590; C:bacterial nucleoid; IEA:UniProtKB-UniRule.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.1310.10; -; 1.
DR HAMAP; MF_00274; DNA_YbaB_EbfC; 1.
DR InterPro; IPR036894; YbaB-like_sf.
DR InterPro; IPR004401; YbaB/EbfC.
DR Pfam; PF02575; YbaB_DNA_bd; 1.
DR PIRSF; PIRSF004555; UCP004555; 1.
DR SUPFAM; SSF82607; SSF82607; 1.
DR TIGRFAMs; TIGR00103; DNA_YbaB_EbfC; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Reference proteome.
FT CHAIN 1..99
FT /note="Nucleoid-associated protein EbfC"
FT /id="PRO_0000170367"
FT MUTAGEN 16
FT /note="K->A: Forms homodimers, but does not bind DNA."
FT /evidence="ECO:0000269|PubMed:19208644"
FT MUTAGEN 20
FT /note="D->A: Forms homodimers, but does not bind DNA."
FT /evidence="ECO:0000269|PubMed:19208644"
FT MUTAGEN 23
FT /note="K->A: Forms homodimers, but does not bind DNA."
FT /evidence="ECO:0000269|PubMed:19208644"
FT MUTAGEN 77
FT /note="N->A: Does not dimerize and does not bind DNA."
FT /evidence="ECO:0000269|PubMed:19208644"
FT MUTAGEN 78
FT /note="D->A: Forms homodimers, but does not bind DNA."
FT /evidence="ECO:0000269|PubMed:19208644"
FT MUTAGEN 82
FT /note="K->A: Forms homodimers, but does not bind DNA."
FT /evidence="ECO:0000269|PubMed:19208644"
FT MUTAGEN 84
FT /note="K->A: Forms homodimers, but does not bind DNA."
FT /evidence="ECO:0000269|PubMed:19208644"
FT MUTAGEN 85
FT /note="E->A: Forms homodimers, but does not bind DNA."
FT /evidence="ECO:0000269|PubMed:19208644"
FT MUTAGEN 88
FT /note="K->A: Forms homodimers, but does not bind DNA."
FT /evidence="ECO:0000269|PubMed:19208644"
SQ SEQUENCE 99 AA; 11017 MW; D38CBBD34E03944F CRC64;
MAVNPLDFLK NMSSVKNNID NIKKEISKIT VCGKAGSNIV TIEMDGEFNV KKVSINKEFF
DDLDNDAFEQ MIKSALNDAV SKVKEEIKLK TMGVLPFGM
