ID   EBGC_ECOLI              Reviewed;         149 AA.
AC   P0AC73; P24225; Q2M9C9;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Evolved beta-galactosidase subunit beta;
GN   Name=ebgC; OrderedLocusNames=b3077, JW3048;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2515108; DOI=10.1093/genetics/123.4.635;
RA   Hall B.G., Betts P.W., Wootton J.C.;
RT   "DNA sequence analysis of artificially evolved ebg enzyme and ebg repressor
RT   genes.";
RL   Genetics 123:635-648(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   SUBUNIT.
RX   PubMed=1540130; DOI=10.1042/bj2820155;
RA   Elliott A.C., Sinnott M.L., Smith P.J., Bommuswamy J., Guo Z., Hall B.G.,
RA   Zhang Y.;
RT   "The catalytic consequences of experimental evolution. Studies on the
RT   subunit structure of the second (ebg) beta-galactosidase of Escherichia
RT   coli, and on catalysis by ebgab, an experimental evolvant containing two
RT   amino acid substitutions.";
RL   Biochem. J. 282:155-164(1992).
CC   -!- FUNCTION: Required for full activity of the EbgA enzyme. Exact function
CC       not known.
CC   -!- SUBUNIT: Heterooctamer of 4 alpha and 4 beta subunits.
CC       {ECO:0000269|PubMed:1540130}.
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DR   EMBL; M64441; AAA61972.1; -; Genomic_DNA.
DR   EMBL; X52031; CAA36275.1; -; Genomic_DNA.
DR   EMBL; U18997; AAA57878.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76112.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77127.1; -; Genomic_DNA.
DR   PIR; B65096; B65096.
DR   RefSeq; NP_417548.1; NC_000913.3.
DR   RefSeq; WP_001219954.1; NZ_STEB01000001.1.
DR   AlphaFoldDB; P0AC73; -.
DR   SMR; P0AC73; -.
DR   BioGRID; 4262400; 5.
DR   BioGRID; 851897; 1.
DR   DIP; DIP-2894N; -.
DR   IntAct; P0AC73; 6.
DR   STRING; 511145.b3077; -.
DR   PaxDb; P0AC73; -.
DR   PRIDE; P0AC73; -.
DR   EnsemblBacteria; AAC76112; AAC76112; b3077.
DR   EnsemblBacteria; BAE77127; BAE77127; BAE77127.
DR   GeneID; 66673024; -.
DR   GeneID; 947581; -.
DR   KEGG; ecj:JW3048; -.
DR   KEGG; eco:b3077; -.
DR   PATRIC; fig|1411691.4.peg.3653; -.
DR   EchoBASE; EB0249; -.
DR   eggNOG; COG2731; Bacteria.
DR   HOGENOM; CLU_146670_0_0_6; -.
DR   OMA; KWNRCVE; -.
DR   BioCyc; EcoCyc:EG10253-MON; -.
DR   SABIO-RK; P0AC73; -.
DR   PRO; PR:P0AC73; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   Gene3D; 2.60.120.370; -; 1.
DR   InterPro; IPR004375; NanQ/TabA/YiaL.
DR   InterPro; IPR037012; NanQ/TabA/YiaL_sf.
DR   PANTHER; PTHR34986; PTHR34986; 1.
DR   Pfam; PF04074; DUF386; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..149
FT                   /note="Evolved beta-galactosidase subunit beta"
FT                   /id="PRO_0000057652"
FT   CONFLICT        144..149
FT                   /note="GYFHNK -> VISITNNNYGGKRSLPPPLPYSFSEMCYV (in Ref. 1;
FT                   AAA61972/CAA36275)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   149 AA;  17457 MW;  5C3CD86D3CB92BB8 CRC64;
     MRIIDNLEQF RQIYASGKKW QRCVEAIENI DNIQPGVAHS IGDSLTYRVE TDSATDALFT
     GHRRYFEVHY YLQGQQKIEY APKETLQVVE YYRDETDREY LKGCGETVEV HEGQIVICDI
     HEAYRFICNN AVKKVVLKVT IEDGYFHNK