ID   EBGR_ECOLI              Reviewed;         327 AA.
AC   P06846; Q2M9D1;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 2.
DT   25-MAY-2022, entry version 153.
DE   RecName: Full=HTH-type transcriptional regulator EbgR;
DE   AltName: Full=Ebg operon repressor;
GN   Name=ebgR; OrderedLocusNames=b3075, JW3046;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2515108; DOI=10.1093/genetics/123.4.635;
RA   Hall B.G., Betts P.W., Wootton J.C.;
RT   "DNA sequence analysis of artificially evolved ebg enzyme and ebg repressor
RT   genes.";
RL   Genetics 123:635-648(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3939708; DOI=10.1093/oxfordjournals.molbev.a040373;
RA   Stokes H.W., Hall B.G.;
RT   "Sequence of the ebgR gene of Escherichia coli: evidence that the EBG and
RT   LAC operons are descended from a common ancestor.";
RL   Mol. Biol. Evol. 2:478-483(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Repressor for beta galactosidase alpha and beta subunits
CC       (ebgA and ebgC). Binds lactose as an inducer.
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DR   EMBL; M64441; AAA61970.1; -; Genomic_DNA.
DR   EMBL; X52031; CAA36273.1; -; Genomic_DNA.
DR   EMBL; U18997; AAA57876.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76110.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77125.1; -; Genomic_DNA.
DR   PIR; A25752; RPECEG.
DR   RefSeq; NP_417546.1; NC_000913.3.
DR   RefSeq; WP_000212475.1; NZ_LN832404.1.
DR   AlphaFoldDB; P06846; -.
DR   SMR; P06846; -.
DR   BioGRID; 4260843; 14.
DR   DIP; DIP-9488N; -.
DR   IntAct; P06846; 1.
DR   STRING; 511145.b3075; -.
DR   jPOST; P06846; -.
DR   PaxDb; P06846; -.
DR   PRIDE; P06846; -.
DR   EnsemblBacteria; AAC76110; AAC76110; b3075.
DR   EnsemblBacteria; BAE77125; BAE77125; BAE77125.
DR   GeneID; 947586; -.
DR   KEGG; ecj:JW3046; -.
DR   KEGG; eco:b3075; -.
DR   PATRIC; fig|1411691.4.peg.3655; -.
DR   EchoBASE; EB0250; -.
DR   eggNOG; COG1609; Bacteria.
DR   HOGENOM; CLU_037628_1_2_6; -.
DR   InParanoid; P06846; -.
DR   OMA; VYTEFMG; -.
DR   PhylomeDB; P06846; -.
DR   BioCyc; EcoCyc:PD03254; -.
DR   PRO; PR:P06846; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:EcoCyc.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   CDD; cd01392; HTH_LacI; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR000843; HTH_LacI.
DR   InterPro; IPR046335; LacI/GalR-like_sensor.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   Pfam; PF00356; LacI; 1.
DR   Pfam; PF13377; Peripla_BP_3; 1.
DR   PRINTS; PR00036; HTHLACI.
DR   SMART; SM00354; HTH_LACI; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF53822; SSF53822; 1.
DR   PROSITE; PS00356; HTH_LACI_1; 1.
DR   PROSITE; PS50932; HTH_LACI_2; 1.
PE   4: Predicted;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..327
FT                   /note="HTH-type transcriptional regulator EbgR"
FT                   /id="PRO_0000107943"
FT   DOMAIN          1..57
FT                   /note="HTH lacI-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT   DNA_BIND        4..23
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT   VARIANT         190
FT                   /note="D -> E (broaden the specificity of inducer
FT                   recognition)"
FT   VARIANT         195
FT                   /note="A -> T (broaden the specificity of inducer
FT                   recognition)"
FT   VARIANT         196
FT                   /note="F -> C (broaden the specificity of inducer
FT                   recognition)"
SQ   SEQUENCE   327 AA;  36210 MW;  2A8688B46A00EE03 CRC64;
     MATLKDIAIE AGVSLATVSR VLNDDPTLNV KEETKHRILE IAEKLEYKTS SARKLQTGAV
     NQHHILAIYS YQQELEINDP YYLAIRHGIE TQCEKLGIEL TNCYEHSGLP DIKNVTGILI
     VGKPTPALRA AASALTDNIC FIDFHEPGSG YDAVDIDLAR ISKEIIDFYI NQGVNRIGFI
     GGEDEPGKAD IREVAFAEYG RLKQVVREED IWRGGFSSSS GYELAKQMLA REDYPKALFV
     ASDSIAIGVL RAIHERGLNI PQDISLISVN DIPTARFTFP PLSTVRIHSE MMGSQGVNLV
     YEKARDGRAL PLLVFVPSKL KLRGTTR