EBIII_PETHY
ID EBIII_PETHY Reviewed; 258 AA.
AC F1B281; E4W4E5; E5CZU4;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 25-MAY-2022, entry version 37.
DE RecName: Full=MYB-like transcription factor 4 {ECO:0000303|PubMed:21068208};
DE Short=PhMYB4 {ECO:0000303|PubMed:21068208};
DE AltName: Full=MYB-like protein MYB5d8 {ECO:0000303|Ref.1};
DE AltName: Full=Protein EMISSION OF BENZENOIDS III {ECO:0000303|Ref.2};
DE Short=PhEOBIII {ECO:0000303|Ref.2};
GN Name=MYB4 {ECO:0000303|PubMed:21068208};
GN Synonyms=EOBIII {ECO:0000303|Ref.2}, MYB5d8 {ECO:0000303|Ref.1};
OS Petunia hybrida (Petunia).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia.
OX NCBI_TaxID=4102;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Mitchell;
RA Verdonk J.C., Colquhoun T.A., Van Moerkercke A.N.A.I., Underwood B.A.,
RA Kim J.Y., Haring M.A., Schuurink R.C., Clark D.G.;
RT "Differential Expression of Transcription Factors in Petunia Flowers.";
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Violet 26;
RA Moyal M., Spitzer-Rimon B., Marhevka E., Ovadis M., Vainstein A.;
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL
RP STAGE, TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Mitchell;
RX PubMed=21068208; DOI=10.1093/jxb/erq342;
RA Colquhoun T.A., Kim J.Y., Wedde A.E., Levin L.A., Schmitt K.C.,
RA Schuurink R.C., Clark D.G.;
RT "PhMYB4 fine-tunes the floral volatile signature of Petunia x hybrida
RT through PhC4H.";
RL J. Exp. Bot. 62:1133-1143(2011).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. W115;
RX PubMed=23275577; DOI=10.1105/tpc.112.105247;
RA Spitzer-Rimon B., Farhi M., Albo B., Cna'ani A., Ben Zvi M.M., Masci T.,
RA Edelbaum O., Yu Y., Shklarman E., Ovadis M., Vainstein A.;
RT "The R2R3-MYB-like regulatory factor EOBI, acting downstream of EOBII,
RT regulates scent production by activating ODO1 and structural scent-related
RT genes in petunia.";
RL Plant Cell 24:5089-5105(2012).
CC -!- FUNCTION: Negative regulators of floral scent production by repressing
CC the expression of C4H1 and C4H2, components of the floral volatile
CC benzenoid/phenylpropanoid (FVBP) biosynthetic pathway.
CC {ECO:0000269|PubMed:21068208}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in petal limbs, petal tubes,
CC anthers and stigmas, and, to a lower extent, in stems. Barely detected
CC in roots, sepals and leaves. {ECO:0000269|PubMed:21068208}.
CC -!- DEVELOPMENTAL STAGE: In corollas, accumulates progressively during
CC flower development, from buds to anthesis, with a peak at flower
CC opening, but fades out in senescing flowers.
CC {ECO:0000269|PubMed:21068208}.
CC -!- INDUCTION: Circadian-regulation with peak levels occurring at the end
CC of the light period in flowers. {ECO:0000269|PubMed:21068208}.
CC -!- DISRUPTION PHENOTYPE: Increased expression of C4H1 and C4H2
CC (PubMed:21068208). Higher levels of floral volatile
CC benzenoid/phenylpropanoid (FVBP) compounds derived from p-coumaric acid
CC (isoeugenol and eugenol) (PubMed:21068208).
CC {ECO:0000269|PubMed:21068208}.
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DR EMBL; EU374208; ACB12238.1; -; mRNA.
DR EMBL; GQ449251; ADK12668.1; -; mRNA.
DR EMBL; HM447143; ADX33331.1; -; mRNA.
DR AlphaFoldDB; F1B281; -.
DR SMR; F1B281; -.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IMP:UniProtKB.
DR GO; GO:0010597; P:green leaf volatile biosynthetic process; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Repeat; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..258
FT /note="MYB-like transcription factor 4"
FT /id="PRO_0000451492"
FT DOMAIN 9..61
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 62..116
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 37..61
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 89..112
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT CONFLICT 61
FT /note="L -> S (in Ref. 2; ADK12668)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="T -> S (in Ref. 2; ADK12668)"
FT /evidence="ECO:0000305"
FT CONFLICT 138..139
FT /note="TT -> H (in Ref. 1; ACB12238)"
FT /evidence="ECO:0000305"
FT CONFLICT 146..154
FT /note="NEDIKDQKI -> MKILKIRRS (in Ref. 1; ACB12238)"
FT /evidence="ECO:0000305"
FT CONFLICT 155..162
FT /note="SIKAEFEQ -> NIKTELGT (in Ref. 2; ADK12668)"
FT /evidence="ECO:0000305"
FT CONFLICT 198
FT /note="L -> F (in Ref. 2; ADK12668)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 258 AA; 29171 MW; 5C2754BF46A57EE7 CRC64;
MGRSPCCEKA HTNKGAWTKE EDERLIAYIK AHGEGCWRSL PKAAGLLRCG KSCRLRWINY
LRPDLKRGNF TEDEDELIIK LHSLLGNKWS LIAGRLPGRT DNEIKNYWNT HIRRKLLSRG
IDPTTHRIMN EPSTQKVTTI SFAAGNEDIK DQKISIKAEF EQIKDDEIIS KPIKEQCPDL
NLELKISPPY QQHSDRALQQ STTGSGGAST ICFTCSLGLK NNKGCSCSRN RSMNVAGYDF
LGLKTNGLDY RTLETRTK
