EBP_ORYSJ
ID EBP_ORYSJ Reviewed; 219 AA.
AC Q9FTZ2; A0A0N7KC64; Q0JRF7; Q7F734;
DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase;
DE EC=5.3.3.5;
DE AltName: Full=Cholestenol Delta-isomerase;
DE AltName: Full=Delta(8)-Delta(7) sterol isomerase;
DE Short=D8-D7 sterol isomerase;
GN OrderedLocusNames=Os01g0103600, LOC_Os01g01369;
GN ORFNames=P0436E04.5, P0455C04.26;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Catalyzes the conversion of Delta(8)-sterols to their
CC corresponding Delta(7)-isomers. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=lathosterol = 5alpha-cholest-8-en-3beta-ol;
CC Xref=Rhea:RHEA:15281, ChEBI:CHEBI:16608, ChEBI:CHEBI:17168;
CC EC=5.3.3.5;
CC -!- PATHWAY: Steroid biosynthesis; sterol biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9FTZ2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9FTZ2-2; Sequence=VSP_017657;
CC -!- SIMILARITY: Belongs to the EBP family. {ECO:0000305}.
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DR EMBL; AP002818; BAB16323.1; -; Genomic_DNA.
DR EMBL; AP002969; BAB92148.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF03671.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS69945.1; -; Genomic_DNA.
DR EMBL; AK059848; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK102848; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015617405.1; XM_015761919.1. [Q9FTZ2-1]
DR AlphaFoldDB; Q9FTZ2; -.
DR SMR; Q9FTZ2; -.
DR STRING; 4530.OS01T0103600-02; -.
DR PaxDb; Q9FTZ2; -.
DR PRIDE; Q9FTZ2; -.
DR EnsemblPlants; Os01t0103600-02; Os01t0103600-02; Os01g0103600. [Q9FTZ2-1]
DR GeneID; 4326449; -.
DR Gramene; Os01t0103600-02; Os01t0103600-02; Os01g0103600. [Q9FTZ2-1]
DR KEGG; osa:4326449; -.
DR eggNOG; KOG4826; Eukaryota.
DR HOGENOM; CLU_072128_2_0_1; -.
DR InParanoid; Q9FTZ2; -.
DR OMA; FEGYFAY; -.
DR OrthoDB; 1130914at2759; -.
DR PlantReactome; R-OSA-1119286; Cholesterol biosynthesis II (via 24,25-dihydrolanosterol).
DR PlantReactome; R-OSA-1119370; Sterol biosynthesis.
DR PlantReactome; R-OSA-1119439; Cholesterol biosynthesis III (via desmosterol).
DR PlantReactome; R-OSA-1119559; Cholesterol biosynthesis I.
DR UniPathway; UPA00766; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR ExpressionAtlas; Q9FTZ2; baseline and differential.
DR Genevisible; Q9FTZ2; OS.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000247; F:C-8 sterol isomerase activity; IBA:GO_Central.
DR GO; GO:0047750; F:cholestenol delta-isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0004769; F:steroid delta-isomerase activity; IBA:GO_Central.
DR GO; GO:0016126; P:sterol biosynthetic process; IBA:GO_Central.
DR InterPro; IPR007905; EBP.
DR InterPro; IPR033118; EXPERA.
DR PANTHER; PTHR14207; PTHR14207; 1.
DR PROSITE; PS51751; EXPERA; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Isomerase; Lipid biosynthesis;
KW Lipid metabolism; Membrane; Reference proteome; Steroid biosynthesis;
KW Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..219
FT /note="Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-
FT isomerase"
FT /id="PRO_0000174346"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 58..200
FT /note="EXPERA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01087"
FT VAR_SEQ 137..204
FT /note="AIASGKSYSHILQFTVCLGQLYGCLVYFITAYLDGFNFWTSPFYFWAYFIGA
FT NSSWVVIPTMIAIRSW -> SSFLPFHLTMRTLPLSLSLSLSLSLSLSLSLSLSFPFLT
FT YLTIFSLQMHFSHVFLPFVILLFFFPLSS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_017657"
SQ SEQUENCE 219 AA; 24758 MW; 5D4A60D361FCA071 CRC64;
MGHPHPHPYA PAELHLPGFV PLQLSQAQIL VPYLATSLFL LLAVWLISGR CSRRLSDTDR
WLMCWWAFTG LTHIIIEGTF VFAPNFFSNQ NPSYFDEVWK EYSKGDSRYV ARDPATVTVE
GITAVLEGPA SLLAVYAIAS GKSYSHILQF TVCLGQLYGC LVYFITAYLD GFNFWTSPFY
FWAYFIGANS SWVVIPTMIA IRSWKKICAA FQGEKVKTK
