ID   EBRA_BACAT              Reviewed;         105 AA.
AC   P0CW81; O31792; Q9R9I0;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Multidrug resistance protein EbrA;
GN   Name=ebrA;
OS   Bacillus atrophaeus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1452;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBUNIT.
RC   STRAIN=ATCC 9372 / DSM 675 / NBRC 13721 / NCIMB 8058 / NRS 1221A;
RX   PubMed=10735876; DOI=10.1128/jb.182.8.2307-2310.2000;
RA   Masaoka Y., Ueno Y., Morita Y., Kuroda T., Mizushima T., Tsuchiya T.;
RT   "A two-component multidrug efflux pump, EbrAB, in Bacillus subtilis.";
RL   J. Bacteriol. 182:2307-2310(2000).
CC   -!- FUNCTION: Part of a multidrug efflux pump. Confers resistance to
CC       cationic lipophilic dyes such as ethidium bromide, acriflavine,
CC       pyronine Y and safranin O. The efflux is probably coupled to an influx
CC       of protons. {ECO:0000269|PubMed:10735876}.
CC   -!- SUBUNIT: The efflux pump is composed of EbrA and EbrB.
CC       {ECO:0000305|PubMed:10735876}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
CC       EbrA/EbrB subfamily. {ECO:0000305}.
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DR   EMBL; AB029306; BAA88575.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0CW81; -.
DR   SMR; P0CW81; -.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   InterPro; IPR045324; Small_multidrug_res.
DR   PANTHER; PTHR30561; PTHR30561; 1.
DR   Pfam; PF00893; Multi_Drug_Res; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..105
FT                   /note="Multidrug resistance protein EbrA"
FT                   /id="PRO_0000408956"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        35..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   105 AA;  11392 MW;  93B4760294D87676 CRC64;
     MLVGYIFLTI AICSESIGAA MLKVSDGFKK WKPSALVVIA YSLAFYMLSL TLNHIPLSLS
     YATWSGVGTV LTAVIGVKWF KEELNAKGLI GILLLISGVV LLNWQ