EBRA_BACLD
ID EBRA_BACLD Reviewed; 105 AA.
AC Q65JB1;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Multidrug resistance protein EbrA;
GN Name=ebrA; OrderedLocusNames=BLi01959, BL05171;
OS Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 /
OS NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=279010;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX PubMed=15383718; DOI=10.1159/000079829;
RA Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P.,
RA Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.;
RT "The complete genome sequence of Bacillus licheniformis DSM13, an organism
RT with great industrial potential.";
RL J. Mol. Microbiol. Biotechnol. 7:204-211(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX PubMed=15461803; DOI=10.1186/gb-2004-5-10-r77;
RA Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J.,
RA Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B.,
RA Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A.,
RA Bolotin A., Lapidus A., Galleron N., Ehrlich S.D., Berka R.M.;
RT "Complete genome sequence of the industrial bacterium Bacillus
RT licheniformis and comparisons with closely related Bacillus species.";
RL Genome Biol. 5:R77.1-R77.12(2004).
CC -!- FUNCTION: Part of a multidrug efflux pump. Confers resistance to
CC cationic lipophilic dyes such as ethidium bromide, acriflavine,
CC pyronine Y and safranin O. The efflux is probably coupled to an influx
CC of protons (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: The efflux pump is composed of EbrA and EbrB. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
CC EbrA/EbrB subfamily. {ECO:0000305}.
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DR EMBL; AE017333; AAU40853.1; -; Genomic_DNA.
DR EMBL; CP000002; AAU23490.1; -; Genomic_DNA.
DR AlphaFoldDB; Q65JB1; -.
DR SMR; Q65JB1; -.
DR STRING; 279010.BL05171; -.
DR EnsemblBacteria; AAU23490; AAU23490; BL05171.
DR KEGG; bld:BLi01959; -.
DR KEGG; bli:BL05171; -.
DR eggNOG; COG2076; Bacteria.
DR HOGENOM; CLU_133067_0_2_9; -.
DR OMA; KWKPSAL; -.
DR Proteomes; UP000000606; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR InterPro; IPR045324; Small_multidrug_res.
DR PANTHER; PTHR30561; PTHR30561; 1.
DR Pfam; PF00893; Multi_Drug_Res; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..105
FT /note="Multidrug resistance protein EbrA"
FT /id="PRO_0000108090"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..104
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 105 AA; 11265 MW; 057C8A128D6BBBDD CRC64;
MIAGYIFLLI AILSEAAAAA MLKISDGFAR WQPSVLVVIG YGLAFYMMSL TLQVIPLSLS
YATWSGAGTV LTAIIGVLWF QEKLNRRNIA GIICLVSGVV LINLS
