EBR_ECOLX
ID EBR_ECOLX Reviewed; 115 AA.
AC P0AA22; P14502;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Putative ethidium bromide resistance protein;
DE AltName: Full=E1 protein;
GN Name=ebr; Synonyms=E1;
OS Escherichia coli.
OG Plasmid pLMO20, Plasmid pLMO27, and Plasmid IncFII NR79.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pLMO20;
RX PubMed=3054482; DOI=10.1007/bf00339581;
RA Sundstroem L., Radstroem P., Swedberg G., Skoeld O.;
RT "Site-specific recombination promotes linkage between trimethoprim- and
RT sulfonamide resistance genes. Sequence characterization of dhfrV and sulI
RT and a recombination active locus of Tn21.";
RL Mol. Gen. Genet. 213:191-201(1988).
RN [2]
RP SEQUENCE REVISION.
RC PLASMID=pLMO20;
RA Sundstroem L.;
RL Submitted (NOV-1989) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFII NR79; TRANSPOSON=Tn2424;
RX PubMed=1314803; DOI=10.1128/jb.174.9.2891-2897.1992;
RA Parent R., Roy P.H.;
RT "The chloramphenicol acetyltransferase gene of Tn2424: a new breed of
RT cat.";
RL J. Bacteriol. 174:2891-2897(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pLMO27; TRANSPOSON=Tn5086;
RX PubMed=8195081; DOI=10.1128/jb.176.11.3257-3268.1994;
RA Raadstroem P., Skoeld O., Swedberg G., Flensburg J., Roy P.H.,
RA Sundstroem L.;
RT "Transposon Tn5090 of plasmid R751, which carries an integron, is related
RT to Tn7, Mu, and the retroelements.";
RL J. Bacteriol. 176:3257-3268(1994).
CC -!- FUNCTION: One of the determinants for resistance to ethidium bromide
CC and quaternary ammonium compounds.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
CC {ECO:0000305}.
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DR EMBL; X12870; CAB37322.1; -; Genomic_DNA.
DR EMBL; X12868; CAA31357.1; -; Genomic_DNA.
DR EMBL; AF047479; AAC14738.1; -; Genomic_DNA.
DR EMBL; X58425; CAA41327.1; -; Genomic_DNA.
DR PIR; B41857; B41857.
DR RefSeq; NP_863001.1; NC_004998.1.
DR RefSeq; WP_000679427.1; NZ_WWEL01000045.1.
DR RefSeq; YP_001096355.1; NC_009132.1.
DR RefSeq; YP_001096412.1; NC_009133.1.
DR RefSeq; YP_001816599.1; NC_010558.1.
DR RefSeq; YP_002527544.1; NC_011964.1.
DR RefSeq; YP_002891163.1; NC_012690.1.
DR RefSeq; YP_002894491.1; NC_012692.1.
DR RefSeq; YP_003937680.1; NC_014615.1.
DR RefSeq; YP_003937695.1; NC_014615.1.
DR RefSeq; YP_004558214.1; NC_015599.1.
DR RefSeq; YP_006903339.1; NC_019045.2.
DR RefSeq; YP_006939956.1; NC_018994.1.
DR RefSeq; YP_006952406.1; NC_019062.1.
DR RefSeq; YP_006952977.1; NC_019066.1.
DR RefSeq; YP_006953196.1; NC_019069.1.
DR RefSeq; YP_006953611.1; NC_019081.1.
DR RefSeq; YP_006953619.1; NC_019082.1.
DR RefSeq; YP_006953870.1; NC_019089.1.
DR RefSeq; YP_006953992.1; NC_019091.1.
DR RefSeq; YP_008574824.1; NC_022374.1.
DR RefSeq; YP_009023103.1; NC_023909.1.
DR RefSeq; YP_009061086.1; NC_024975.1.
DR RefSeq; YP_009061436.1; NC_024978.1.
DR RefSeq; YP_009061693.1; NC_024980.1.
DR RefSeq; YP_009066445.1; NC_025106.1.
DR RefSeq; YP_009068293.1; NC_025139.1.
DR RefSeq; YP_009070795.1; NC_025175.1.
DR RefSeq; YP_009182146.1; NC_028464.1.
DR RefSeq; YP_724470.1; NC_007682.3.
DR AlphaFoldDB; P0AA22; -.
DR SMR; P0AA22; -.
DR GeneID; 67375552; -.
DR OMA; ATTAMKY; -.
DR OrthoDB; 1921902at2; -.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR InterPro; IPR045324; Small_multidrug_res.
DR PANTHER; PTHR30561; PTHR30561; 1.
DR Pfam; PF00893; Multi_Drug_Res; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Plasmid; Transmembrane; Transmembrane helix;
KW Transport; Transposable element.
FT CHAIN 1..115
FT /note="Putative ethidium bromide resistance protein"
FT /id="PRO_0000108071"
FT TRANSMEM 4..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..104
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 115 AA; 12332 MW; 1676BE6C66759B5E CRC64;
MKGWLFLVIA IVGEVIATSA LKSSEGFTKL APSAVVIIGY GIAFYFLSLV LKSIPVGVAY
AVWSGLGVVI ITAIAWLLHG QKLDAWGFVG MGLIIAAFLL ARSPSWKSLR RPTPW
