ID   EBR_PSEAI               Reviewed;         115 AA.
AC   P0AA24; P14502;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Putative ethidium bromide resistance protein;
DE   AltName: Full=E1 protein;
GN   Name=ebr; Synonyms=E1;
OS   Pseudomonas aeruginosa.
OG   Plasmid pVS1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1310501; DOI=10.1128/jb.174.4.1248-1257.1992;
RA   Bissonnette L., Roy P.H.;
RT   "Characterization of In0 of Pseudomonas aeruginosa plasmid pVS1, an
RT   ancestor of integrons of multiresistance plasmids and transposons of Gram-
RT   negative bacteria.";
RL   J. Bacteriol. 174:1248-1257(1992).
CC   -!- FUNCTION: One of the determinants for resistance to ethidium bromide
CC       and quaternary ammonium compounds.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
CC       {ECO:0000305}.
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DR   EMBL; U49101; AAC44316.1; -; Genomic_DNA.
DR   EMBL; M73819; AAA25858.1; -; Genomic_DNA.
DR   PIR; B42646; B42646.
DR   RefSeq; WP_000679427.1; NZ_WXZU01000048.1.
DR   RefSeq; YP_007509600.1; NC_020452.1.
DR   RefSeq; YP_245439.1; NC_007100.1.
DR   AlphaFoldDB; P0AA24; -.
DR   SMR; P0AA24; -.
DR   GeneID; 67375552; -.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   InterPro; IPR045324; Small_multidrug_res.
DR   PANTHER; PTHR30561; PTHR30561; 1.
DR   Pfam; PF00893; Multi_Drug_Res; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Plasmid; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..115
FT                   /note="Putative ethidium bromide resistance protein"
FT                   /id="PRO_0000108073"
FT   TRANSMEM        4..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        30..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   115 AA;  12332 MW;  1676BE6C66759B5E CRC64;
     MKGWLFLVIA IVGEVIATSA LKSSEGFTKL APSAVVIIGY GIAFYFLSLV LKSIPVGVAY
     AVWSGLGVVI ITAIAWLLHG QKLDAWGFVG MGLIIAAFLL ARSPSWKSLR RPTPW