EBS1_YEAST
ID EBS1_YEAST Reviewed; 884 AA.
AC Q03466; D6VSI7;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Nonsense-mediated mRNA decay factor EBS1 {ECO:0000305};
DE AltName: Full=EST1-like BCY1 suppressor 1 {ECO:0000303|PubMed:10688642};
GN Name=EBS1 {ECO:0000303|PubMed:10688642};
GN OrderedLocusNames=YDR206W {ECO:0000312|SGD:S000002614}; ORFNames=YD8142.03;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=10688642; DOI=10.1128/mcb.20.6.1947-1955.2000;
RA Zhou J., Hidaka K., Futcher B.;
RT "The Est1 subunit of yeast telomerase binds the Tlc1 telomerase RNA.";
RL Mol. Cell. Biol. 20:1947-1955(2000).
RN [4]
RP FUNCTION, AND INTERACTION WITH CDC33.
RX PubMed=16467471; DOI=10.1128/ec.5.2.301-312.2006;
RA Ford A.S., Guan Q., Neeno-Eckwall E., Culbertson M.R.;
RT "Ebs1p, a negative regulator of gene expression controlled by the Upf
RT proteins in the yeast Saccharomyces cerevisiae.";
RL Eukaryot. Cell 5:301-312(2006).
RN [5]
RP FUNCTION, INTERACTION WITH UPF1, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=17984081; DOI=10.1093/nar/gkm912;
RA Luke B., Azzalin C.M., Hug N., Deplazes A., Peter M., Lingner J.;
RT "Saccharomyces cerevisiae Ebs1p is a putative ortholog of human Smg7 and
RT promotes nonsense-mediated mRNA decay.";
RL Nucleic Acids Res. 35:7688-7697(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=29897761; DOI=10.1021/acs.jproteome.8b00032;
RA He C., Jia C., Zhang Y., Xu P.;
RT "Enrichment-based proteogenomics identifies microproteins, missing
RT proteins, and novel smORFs in Saccharomyces cerevisiae.";
RL J. Proteome Res. 17:2335-2344(2018).
CC -!- FUNCTION: Plays a role in nonsense-mediated mRNA decay (NMD)
CC (PubMed:16467471, PubMed:17984081). Recruits UPF1 to cytoplasmic mRNA
CC decay bodies (P-bodies) (PubMed:17984081). Negative regulator of gene
CC expression. Inhibits translation most likely through effects on eIF-4E
CC (CDC33) (PubMed:16467471). Involved in telomere maintenance
CC (PubMed:10688642). {ECO:0000269|PubMed:10688642,
CC ECO:0000269|PubMed:16467471, ECO:0000269|PubMed:17984081}.
CC -!- SUBUNIT: Interacts with NMD helicase UPF1 (PubMed:17984081). Interacts
CC with CDC33 (PubMed:16467471). {ECO:0000269|PubMed:16467471,
CC ECO:0000269|PubMed:17984081}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. Chromosome, telomere
CC {ECO:0000305}. Cytoplasm, P-body {ECO:0000269|PubMed:17984081}.
CC -!- DISRUPTION PHENOTYPE: Results in stabilization of NMD targets.
CC {ECO:0000269|PubMed:17984081}.
CC -!- SIMILARITY: Belongs to the EST1 family. {ECO:0000305}.
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DR EMBL; Z68194; CAA92345.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12047.1; -; Genomic_DNA.
DR PIR; S61569; S61569.
DR RefSeq; NP_010492.3; NM_001180514.3.
DR AlphaFoldDB; Q03466; -.
DR SMR; Q03466; -.
DR BioGRID; 32256; 103.
DR DIP; DIP-1297N; -.
DR IntAct; Q03466; 15.
DR MINT; Q03466; -.
DR STRING; 4932.YDR206W; -.
DR iPTMnet; Q03466; -.
DR MaxQB; Q03466; -.
DR PaxDb; Q03466; -.
DR PRIDE; Q03466; -.
DR EnsemblFungi; YDR206W_mRNA; YDR206W; YDR206W.
DR GeneID; 851787; -.
DR KEGG; sce:YDR206W; -.
DR SGD; S000002614; EBS1.
DR VEuPathDB; FungiDB:YDR206W; -.
DR eggNOG; KOG2162; Eukaryota.
DR GeneTree; ENSGT00940000176623; -.
DR HOGENOM; CLU_010068_0_0_1; -.
DR InParanoid; Q03466; -.
DR OMA; EIIEYYF; -.
DR BioCyc; YEAST:G3O-29790-MON; -.
DR Reactome; R-SCE-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:Q03466; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q03466; protein.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000932; C:P-body; IDA:SGD.
DR GO; GO:0006310; P:DNA recombination; IMP:SGD.
DR GO; GO:0017148; P:negative regulation of translation; IMP:SGD.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IMP:SGD.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR018834; DNA/RNA-bd_Est1-type.
DR InterPro; IPR045153; Est1/Ebs1-like.
DR InterPro; IPR019458; Est1_N.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR15696; PTHR15696; 1.
DR Pfam; PF10374; EST1; 1.
DR Pfam; PF10373; EST1_DNA_bind; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
PE 1: Evidence at protein level;
KW Chromosome; Cytoplasm; Nonsense-mediated mRNA decay; Nucleus;
KW Reference proteome; Telomere.
FT CHAIN 1..884
FT /note="Nonsense-mediated mRNA decay factor EBS1"
FT /id="PRO_0000086907"
FT REGION 596..645
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 755..774
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 614..645
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 884 AA; 100000 MW; 14482D64E32A1860 CRC64;
MEPSNTQKED LPTAFNGIKS QLNSILKSNQ LFQDYALLNG FLAFVHSKLN AAILTSIESQ
CGKSFAADLD SFDQSSISSI LDFSWESVHY PIFKWFQMWR NYILFEKENK KQQTKFIDFR
KMNSKMLKFF KTVQNFYVNV INTVYKKYDI SVLLPKRIIQ DLKLSDIENT TNVGDILAVK
TFNSSSPLAH LIPTLFHRCL LFLGTAYRYK TLLEEISNKY SISNFKKSLD FFRLASLVLP
SAGETYSQAG AIFLQTGNLG IAVFNFVKGM MTKMPSPVSI KNFGALMVDN KSSLNRSLHT
TIMNTYLQES KGPRTPAKEI LEFYFLGLFG SVWSPTSWRD DTKPNQLNNG IKLRHLENAL
YETMSARYLN NIKTIFHNLI ITIGGFHLLL KRRSDVSAKT LKDLRSNELD YLNFAFKYIA
HILNDIVKES WSENPEVSEI LGMVRIINCW IKANPMVLQY SQSNLEFVNA LAYLINDIVK
KKPSPSFSIT EHIPKRTYWF EEDLMVKGLS FVNFQLSDFD DYEKILEMDH SLDRLIGNPP
LCDKLSASSE MLLRLQAVVN ISSQLLQNNN CGVEWSDNKS RYIFNKKIGF KETVKNSMKT
SKQSNEKAKL QRKNKPSTTN GSISMADLER QMRSSSLDSF SPTMGYSGSS VPMAPDTFNV
KPSGTITGNK VNVELLKIEL SGQNADGAIT NISPGYSNAA ISSSNSTDES SFDLNNILSS
MQQKHAEKSF AKSMQGVNEQ IPANDVCHQA QRPMQGGLYS SQQPSSMSSL NSAYQNASMP
PSASMVSYPY PFLNQQGQGV FPPYNAQNLQ WQSEAYSLKS MNFANPTWLG DQYQTSAPSS
AYAQAQRQMF QQPMQQDVGK YMQFPFDAQS NTDSMRGNSR NNMF
