ID   EC11_ARATH              Reviewed;         158 AA.
AC   Q9SRD8;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Egg cell-secreted protein 1.1;
DE   Flags: Precursor;
GN   Name=EC1.1; OrderedLocusNames=At1g76750; ORFNames=F28O16.12;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Xiao Y.-L., Underwood B.A., Moskal W.A. Jr., Wang W., Redman J.C., Wu H.C.,
RA   Utterback T., Town C.D.;
RT   "Reconstruction of cDNA sequences for hypothetical genes in Arabidopsis
RT   thaliana from 5' and 3' RACE products.";
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RA   Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA   Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=23180860; DOI=10.1126/science.1223944;
RA   Sprunck S., Rademacher S., Vogler F., Gheyselinck J., Grossniklaus U.,
RA   Dresselhaus T.;
RT   "Egg cell-secreted EC1 triggers sperm cell activation during double
RT   fertilization.";
RL   Science 338:1093-1097(2012).
CC   -!- FUNCTION: Involved in the regulation of gamete interactions during the
CC       double fertilization and to prevent multiple-pollen tube attraction;
CC       mediates the redistribution of the gamete fusogen HAP2/GCS1 to the cell
CC       surface after secretion upon sperm arrival.
CC       {ECO:0000269|PubMed:23180860}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle
CC       {ECO:0000269|PubMed:23180860}. Secreted {ECO:0000269|PubMed:23180860}.
CC       Note=Secreted via vesicle exocytose upon sperm arrival, especially in
CC       the apical region of the degenerating synergid cell.
CC   -!- TISSUE SPECIFICITY: Restricted to female reproductive tissues,
CC       specifically accumulating in storage vesicles of the unfertilized egg
CC       cell. {ECO:0000269|PubMed:23180860}.
CC   -!- DEVELOPMENTAL STAGE: Confined to the egg cell before fertilization, but
CC       disappears upon gamete fusion. Also present in zygotes and early
CC       embryos. {ECO:0000269|PubMed:23180860}.
CC   -!- SIMILARITY: Belongs to the plant egg cell-secreted peptide family.
CC       {ECO:0000305}.
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DR   EMBL; AC010718; AAF04438.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE35885.1; -; Genomic_DNA.
DR   EMBL; AY735569; AAU44439.1; -; mRNA.
DR   EMBL; AY773832; AAV63861.1; -; Genomic_DNA.
DR   PIR; A96796; A96796.
DR   RefSeq; NP_177801.1; NM_106325.2.
DR   AlphaFoldDB; Q9SRD8; -.
DR   STRING; 3702.AT1G76750.1; -.
DR   PaxDb; Q9SRD8; -.
DR   PRIDE; Q9SRD8; -.
DR   DNASU; 844009; -.
DR   EnsemblPlants; AT1G76750.1; AT1G76750.1; AT1G76750.
DR   GeneID; 844009; -.
DR   Gramene; AT1G76750.1; AT1G76750.1; AT1G76750.
DR   KEGG; ath:AT1G76750; -.
DR   Araport; AT1G76750; -.
DR   TAIR; locus:2030136; AT1G76750.
DR   eggNOG; ENOG502S3PF; Eukaryota.
DR   HOGENOM; CLU_128969_2_0_1; -.
DR   InParanoid; Q9SRD8; -.
DR   OMA; CDHETSK; -.
DR   OrthoDB; 1573060at2759; -.
DR   PhylomeDB; Q9SRD8; -.
DR   PRO; PR:Q9SRD8; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9SRD8; baseline and differential.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0031982; C:vesicle; IDA:UniProtKB.
DR   GO; GO:0009567; P:double fertilization forming a zygote and endosperm; IGI:TAIR.
DR   GO; GO:0080155; P:regulation of double fertilization forming a zygote and endosperm; IMP:UniProtKB.
DR   GO; GO:2000008; P:regulation of protein localization to cell surface; IMP:UniProtKB.
DR   GO; GO:0048240; P:sperm capacitation; IDA:TAIR.
DR   InterPro; IPR044711; EC11/15.
DR   InterPro; IPR008502; Prolamin-like.
DR   PANTHER; PTHR35293; PTHR35293; 1.
DR   Pfam; PF05617; Prolamin_like; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Fertilization; Glycoprotein; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..158
FT                   /note="Egg cell-secreted protein 1.1"
FT                   /id="PRO_0000421241"
FT   CARBOHYD        122
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   158 AA;  16998 MW;  01B02D9B062C3E8E CRC64;
     MASKSSFMAT FNIVTLMLMV ASSTVTARPL MKPSMGTSSP TTSLVYRLKL DEDTGYCWDS
     LMQLQHCSGE LILFFLNGET YIGPGCCSAI RTIGRKCWPT MIGVLGFTAQ EGDMLQGYCD
     GNDSDNNGED HALASSTLPL SVNFKTTVVR SSASPSNP