ID   EC12_ARATH              Reviewed;         125 AA.
AC   Q9SJ24; A0MEN9;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Egg cell-secreted protein 1.2;
DE   Flags: Precursor;
GN   Name=EC1.2; OrderedLocusNames=At2g21740; ORFNames=F7D8.6;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA   Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT   "Simultaneous high-throughput recombinational cloning of open reading
RT   frames in closed and open configurations.";
RL   Plant Biotechnol. J. 4:317-324(2006).
RN   [4]
RP   FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=23180860; DOI=10.1126/science.1223944;
RA   Sprunck S., Rademacher S., Vogler F., Gheyselinck J., Grossniklaus U.,
RA   Dresselhaus T.;
RT   "Egg cell-secreted EC1 triggers sperm cell activation during double
RT   fertilization.";
RL   Science 338:1093-1097(2012).
CC   -!- FUNCTION: Involved in the regulation of gamete interactions during the
CC       double fertilization and to prevent multiple-pollen tube attraction;
CC       mediates the redistribution of the gamete fusogen HAP2/GCS1 to the cell
CC       surface after secretion upon sperm arrival.
CC       {ECO:0000269|PubMed:23180860}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle
CC       {ECO:0000269|PubMed:23180860}. Secreted {ECO:0000269|PubMed:23180860}.
CC       Note=Secreted via vesicle exocytose upon sperm arrival, especially in
CC       the apical region of the degenerating synergid cell.
CC   -!- TISSUE SPECIFICITY: Restricted to female reproductive tissues,
CC       specifically accumulating in storage vesicles of the unfertilized egg
CC       cell. {ECO:0000269|PubMed:23180860}.
CC   -!- DEVELOPMENTAL STAGE: Confined to the egg cell before fertilization, but
CC       disappears upon gamete fusion. Also present in zygotes and early
CC       embryos. {ECO:0000269|PubMed:23180860}.
CC   -!- SIMILARITY: Belongs to the plant egg cell-secreted peptide family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABK28194.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AC007019; AAD20394.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC07218.1; -; Genomic_DNA.
DR   EMBL; DQ446544; ABE65456.1; -; Genomic_DNA.
DR   EMBL; DQ653011; ABK28194.1; ALT_SEQ; Genomic_DNA.
DR   PIR; F84604; F84604.
DR   RefSeq; NP_179766.1; NM_127744.2.
DR   AlphaFoldDB; Q9SJ24; -.
DR   STRING; 3702.AT2G21740.1; -.
DR   PaxDb; Q9SJ24; -.
DR   EnsemblPlants; AT2G21740.1; AT2G21740.1; AT2G21740.
DR   GeneID; 816711; -.
DR   Gramene; AT2G21740.1; AT2G21740.1; AT2G21740.
DR   KEGG; ath:AT2G21740; -.
DR   Araport; AT2G21740; -.
DR   TAIR; locus:2052536; AT2G21740.
DR   eggNOG; ENOG502S3PF; Eukaryota.
DR   HOGENOM; CLU_128969_2_0_1; -.
DR   InParanoid; Q9SJ24; -.
DR   OMA; VITHHCW; -.
DR   OrthoDB; 1598350at2759; -.
DR   PhylomeDB; Q9SJ24; -.
DR   PRO; PR:Q9SJ24; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q9SJ24; baseline and differential.
DR   Genevisible; Q9SJ24; AT.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0031982; C:vesicle; IDA:UniProtKB.
DR   GO; GO:0009567; P:double fertilization forming a zygote and endosperm; IGI:TAIR.
DR   GO; GO:0080155; P:regulation of double fertilization forming a zygote and endosperm; IMP:UniProtKB.
DR   GO; GO:2000008; P:regulation of protein localization to cell surface; IMP:UniProtKB.
DR   InterPro; IPR044707; EC12/13/14.
DR   InterPro; IPR008502; Prolamin-like.
DR   PANTHER; PTHR31181; PTHR31181; 1.
DR   Pfam; PF05617; Prolamin_like; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Fertilization; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..125
FT                   /note="Egg cell-secreted protein 1.2"
FT                   /id="PRO_0000421242"
SQ   SEQUENCE   125 AA;  13303 MW;  C5851A9DB6D42CDF CRC64;
     MASNTSFLFA TIAILLVLNI SGRTLPETED STNIAARLNG GGLMECWNAL YELKSCTNEI
     VLFFLNGETK LGVDCCQAVE VITTDCWPAM LTSLGFTSDE TNVLRGFCQS PNSGGSSPAP
     SSVKL