EC14_ARATH
ID EC14_ARATH Reviewed; 127 AA.
AC Q9T039; A0MFC9;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Egg cell-secreted protein 1.4;
DE Flags: Precursor;
GN Name=EC1.4; OrderedLocusNames=At4g39340; ORFNames=T22F8.240;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR
RP LOCATION.
RX PubMed=23180860; DOI=10.1126/science.1223944;
RA Sprunck S., Rademacher S., Vogler F., Gheyselinck J., Grossniklaus U.,
RA Dresselhaus T.;
RT "Egg cell-secreted EC1 triggers sperm cell activation during double
RT fertilization.";
RL Science 338:1093-1097(2012).
CC -!- FUNCTION: Involved in the regulation of gamete interactions during the
CC double fertilization and to prevent multiple-pollen tube attraction;
CC mediates the redistribution of the gamete fusogen HAP2/GCS1 to the cell
CC surface after secretion upon sperm arrival.
CC {ECO:0000269|PubMed:23180860}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle
CC {ECO:0000269|PubMed:23180860}. Secreted {ECO:0000269|PubMed:23180860}.
CC Note=Secreted via vesicle exocytose upon sperm arrival, especially in
CC the apical region of the degenerating synergid cell.
CC -!- TISSUE SPECIFICITY: Restricted to female reproductive tissues,
CC specifically accumulating in storage vesicles of the unfertilized egg
CC cell. {ECO:0000269|PubMed:23180860}.
CC -!- DEVELOPMENTAL STAGE: Confined to the egg cell before fertilization, but
CC disappears upon gamete fusion. Also present in zygotes and early
CC embryos. {ECO:0000269|PubMed:23180860}.
CC -!- SIMILARITY: Belongs to the plant egg cell-secreted peptide family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28267.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL050351; CAB43649.1; -; Genomic_DNA.
DR EMBL; AL161595; CAB80597.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE87058.1; -; Genomic_DNA.
DR EMBL; DQ446907; ABE65542.1; -; Genomic_DNA.
DR EMBL; DQ653255; ABK28267.1; ALT_SEQ; Genomic_DNA.
DR PIR; T08582; T08582.
DR RefSeq; NP_195644.1; NM_120094.1.
DR AlphaFoldDB; Q9T039; -.
DR STRING; 3702.AT4G39340.1; -.
DR PaxDb; Q9T039; -.
DR ProteomicsDB; 222052; -.
DR EnsemblPlants; AT4G39340.1; AT4G39340.1; AT4G39340.
DR GeneID; 830089; -.
DR Gramene; AT4G39340.1; AT4G39340.1; AT4G39340.
DR KEGG; ath:AT4G39340; -.
DR Araport; AT4G39340; -.
DR TAIR; locus:2136293; AT4G39340.
DR eggNOG; ENOG502S3PF; Eukaryota.
DR HOGENOM; CLU_128969_2_0_1; -.
DR InParanoid; Q9T039; -.
DR OMA; GFCQNPN; -.
DR OrthoDB; 1598350at2759; -.
DR PhylomeDB; Q9T039; -.
DR PRO; PR:Q9T039; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9T039; baseline and differential.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0031982; C:vesicle; IDA:UniProtKB.
DR GO; GO:0009567; P:double fertilization forming a zygote and endosperm; IGI:TAIR.
DR GO; GO:0080155; P:regulation of double fertilization forming a zygote and endosperm; IMP:UniProtKB.
DR GO; GO:2000008; P:regulation of protein localization to cell surface; IMP:UniProtKB.
DR InterPro; IPR044707; EC12/13/14.
DR InterPro; IPR008502; Prolamin-like.
DR PANTHER; PTHR31181; PTHR31181; 1.
DR Pfam; PF05617; Prolamin_like; 1.
PE 2: Evidence at transcript level;
KW Cytoplasmic vesicle; Fertilization; Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..127
FT /note="Egg cell-secreted protein 1.4"
FT /id="PRO_0000421244"
SQ SEQUENCE 127 AA; 13613 MW; B4405F927C05D595 CRC64;
MASNTTFLFS TVTLLIILLN TTVSGRDLPA ESSTNIAARL QSGGLMECWN ALYELKSCTN
EIVLFFLNGE TKLGVSCCES VDIITTNCWP AMLTSLGFTP EEANVLRGFC QNPNSGDSSP
APSPKIV
