EC15_ARATH
ID EC15_ARATH Reviewed; 155 AA.
AC Q9FGG1;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Egg cell-secreted protein 1.5;
DE Flags: Precursor;
GN Name=EC1.5; OrderedLocusNames=At5g64720; ORFNames=MVP7.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR
RP LOCATION.
RX PubMed=23180860; DOI=10.1126/science.1223944;
RA Sprunck S., Rademacher S., Vogler F., Gheyselinck J., Grossniklaus U.,
RA Dresselhaus T.;
RT "Egg cell-secreted EC1 triggers sperm cell activation during double
RT fertilization.";
RL Science 338:1093-1097(2012).
CC -!- FUNCTION: Involved in the regulation of gamete interactions during the
CC double fertilization and to prevent multiple-pollen tube attraction;
CC mediates the redistribution of the gamete fusogen HAP2/GCS1 to the cell
CC surface after secretion upon sperm arrival.
CC {ECO:0000269|PubMed:23180860}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle
CC {ECO:0000269|PubMed:23180860}. Secreted {ECO:0000269|PubMed:23180860}.
CC Note=Secreted via vesicle exocytose upon sperm arrival, especially in
CC the apical region of the degenerating synergid cell.
CC -!- TISSUE SPECIFICITY: Restricted to female reproductive tissues,
CC specifically accumulating in storage vesicles of the unfertilized egg
CC cell. {ECO:0000269|PubMed:23180860}.
CC -!- DEVELOPMENTAL STAGE: Confined to the egg cell before fertilization, but
CC disappears upon gamete fusion. Also present in zygotes and early
CC embryos. {ECO:0000269|PubMed:23180860}.
CC -!- SIMILARITY: Belongs to the plant egg cell-secreted peptide family.
CC {ECO:0000305}.
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DR EMBL; AB025637; BAB10305.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97943.1; -; Genomic_DNA.
DR EMBL; BT029327; ABK32141.1; -; mRNA.
DR RefSeq; NP_201277.3; NM_125868.4.
DR AlphaFoldDB; Q9FGG1; -.
DR STRING; 3702.AT5G64720.1; -.
DR PaxDb; Q9FGG1; -.
DR PRIDE; Q9FGG1; -.
DR ProteomicsDB; 222053; -.
DR DNASU; 836593; -.
DR EnsemblPlants; AT5G64720.1; AT5G64720.1; AT5G64720.
DR GeneID; 836593; -.
DR Gramene; AT5G64720.1; AT5G64720.1; AT5G64720.
DR KEGG; ath:AT5G64720; -.
DR Araport; AT5G64720; -.
DR TAIR; locus:2176080; AT5G64720.
DR HOGENOM; CLU_128969_2_0_1; -.
DR InParanoid; Q9FGG1; -.
DR OMA; GGIDKDC; -.
DR OrthoDB; 1423341at2759; -.
DR PhylomeDB; Q9FGG1; -.
DR PRO; PR:Q9FGG1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FGG1; baseline and differential.
DR Genevisible; Q9FGG1; AT.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0031982; C:vesicle; IDA:UniProtKB.
DR GO; GO:0009567; P:double fertilization forming a zygote and endosperm; IGI:TAIR.
DR GO; GO:0080155; P:regulation of double fertilization forming a zygote and endosperm; IMP:UniProtKB.
DR GO; GO:2000008; P:regulation of protein localization to cell surface; IMP:UniProtKB.
DR InterPro; IPR044711; EC11/15.
DR InterPro; IPR008502; Prolamin-like.
DR PANTHER; PTHR35293; PTHR35293; 1.
DR Pfam; PF05617; Prolamin_like; 1.
PE 2: Evidence at transcript level;
KW Cytoplasmic vesicle; Fertilization; Reference proteome; Secreted; Signal.
FT SIGNAL 1..32
FT /evidence="ECO:0000255"
FT CHAIN 33..155
FT /note="Egg cell-secreted protein 1.5"
FT /id="PRO_0000421245"
SQ SEQUENCE 155 AA; 16850 MW; 093A34587D9ED54F CRC64;
MATKSTSKPL LLSFLMMSYL ISTFHVITVA EGRTLQFTKM ATDHSGAGNL MDCWNAGLEL
KSCTDEIVKF FLSQTGTSEP PVKGGIDKDC CGAIGLVVKD CWSVMFTSLG LTTMEGNNLR
EYCEFQAEKS ELSPSPAPET LALSPVEITY PGLDY
