EC1_WHEAT
ID EC1_WHEAT Reviewed; 81 AA.
AC P30569;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=EC protein I/II;
DE AltName: Full=Zinc metallothionein class II;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Neepawa; TISSUE=Embryo;
RX PubMed=1425704; DOI=10.1111/j.1432-1033.1992.tb17370.x;
RA Kawashima I., Kennedy T.D., Chino M., Lane B.G.;
RT "Wheat Ec metallothionein genes. Like mammalian Zn2+ metallothionein genes,
RT wheat Zn2+ metallothionein genes are conspicuously expressed during
RT embryogenesis.";
RL Eur. J. Biochem. 209:971-976(1992).
RN [2]
RP PROTEIN SEQUENCE OF 2-60.
RC STRAIN=cv. Thatcher; TISSUE=Embryo;
RA Hofmann T., Kells D.I.C., Lane B.G.;
RT "Partial amino acid sequence of the wheat germ Ec protein. Comparison with
RT another protein very rich in half-cystine and glycine. Wheat germ
RT agglutinin.";
RL Can. J. Biochem. Cell Biol. 62:908-913(1984).
CC -!- FUNCTION: Binds 5 molecules of zinc. May have a role in Zn(2+)
CC homeostasis during embryogenesis.
CC -!- DEVELOPMENTAL STAGE: Expressed during embryogenesis.
CC -!- INDUCTION: By abscisic acid (ABA).
CC -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 15 family.
CC {ECO:0000305}.
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DR EMBL; X68289; CAA48349.1; -; mRNA.
DR EMBL; X68290; CAA48350.1; -; mRNA.
DR PIR; S27369; S27369.
DR PDB; 2KAK; NMR; -; A=31-81.
DR PDB; 2L61; NMR; -; A=2-25.
DR PDB; 2L62; NMR; -; A=2-25.
DR PDB; 2MFP; NMR; -; A=2-27.
DR PDBsum; 2KAK; -.
DR PDBsum; 2L61; -.
DR PDBsum; 2L62; -.
DR PDBsum; 2MFP; -.
DR AlphaFoldDB; P30569; -.
DR BMRB; P30569; -.
DR SMR; P30569; -.
DR PRIDE; P30569; -.
DR EnsemblPlants; TraesCAD_scaffold_029776_01G000200.1; TraesCAD_scaffold_029776_01G000200.1; TraesCAD_scaffold_029776_01G000200.
DR EnsemblPlants; TraesCAD_scaffold_098637_01G000200.1; TraesCAD_scaffold_098637_01G000200.1; TraesCAD_scaffold_098637_01G000200.
DR EnsemblPlants; TraesCLE_scaffold_055646_01G000400.1; TraesCLE_scaffold_055646_01G000400.1; TraesCLE_scaffold_055646_01G000400.
DR EnsemblPlants; TraesCLE_scaffold_166105_01G000100.1; TraesCLE_scaffold_166105_01G000100.1; TraesCLE_scaffold_166105_01G000100.
DR EnsemblPlants; TraesCS1B02G216800.1; TraesCS1B02G216800.1.cds1; TraesCS1B02G216800.
DR EnsemblPlants; TraesCS1D02G206300.1; TraesCS1D02G206300.1.cds1; TraesCS1D02G206300.
DR EnsemblPlants; TraesPAR_scaffold_114908_01G000100.1; TraesPAR_scaffold_114908_01G000100.1; TraesPAR_scaffold_114908_01G000100.
DR EnsemblPlants; TraesPAR_scaffold_210349_01G000100.1; TraesPAR_scaffold_210349_01G000100.1; TraesPAR_scaffold_210349_01G000100.
DR EnsemblPlants; TraesROB_scaffold_091640_01G000400.1; TraesROB_scaffold_091640_01G000400.1; TraesROB_scaffold_091640_01G000400.
DR EnsemblPlants; TraesROB_scaffold_100017_01G000200.1; TraesROB_scaffold_100017_01G000200.1; TraesROB_scaffold_100017_01G000200.
DR EnsemblPlants; TraesWEE_scaffold_108089_01G000100.1; TraesWEE_scaffold_108089_01G000100.1; TraesWEE_scaffold_108089_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_109422_01G000100.1; TraesWEE_scaffold_109422_01G000100.1; TraesWEE_scaffold_109422_01G000100.
DR Gramene; TraesCAD_scaffold_029776_01G000200.1; TraesCAD_scaffold_029776_01G000200.1; TraesCAD_scaffold_029776_01G000200.
DR Gramene; TraesCAD_scaffold_098637_01G000200.1; TraesCAD_scaffold_098637_01G000200.1; TraesCAD_scaffold_098637_01G000200.
DR Gramene; TraesCLE_scaffold_055646_01G000400.1; TraesCLE_scaffold_055646_01G000400.1; TraesCLE_scaffold_055646_01G000400.
DR Gramene; TraesCLE_scaffold_166105_01G000100.1; TraesCLE_scaffold_166105_01G000100.1; TraesCLE_scaffold_166105_01G000100.
DR Gramene; TraesCS1B02G216800.1; TraesCS1B02G216800.1.cds1; TraesCS1B02G216800.
DR Gramene; TraesCS1D02G206300.1; TraesCS1D02G206300.1.cds1; TraesCS1D02G206300.
DR Gramene; TraesPAR_scaffold_114908_01G000100.1; TraesPAR_scaffold_114908_01G000100.1; TraesPAR_scaffold_114908_01G000100.
DR Gramene; TraesPAR_scaffold_210349_01G000100.1; TraesPAR_scaffold_210349_01G000100.1; TraesPAR_scaffold_210349_01G000100.
DR Gramene; TraesROB_scaffold_091640_01G000400.1; TraesROB_scaffold_091640_01G000400.1; TraesROB_scaffold_091640_01G000400.
DR Gramene; TraesROB_scaffold_100017_01G000200.1; TraesROB_scaffold_100017_01G000200.1; TraesROB_scaffold_100017_01G000200.
DR Gramene; TraesWEE_scaffold_108089_01G000100.1; TraesWEE_scaffold_108089_01G000100.1; TraesWEE_scaffold_108089_01G000100.
DR Gramene; TraesWEE_scaffold_109422_01G000100.1; TraesWEE_scaffold_109422_01G000100.1; TraesWEE_scaffold_109422_01G000100.
DR OMA; GTGCTCV; -.
DR EvolutionaryTrace; P30569; -.
DR Proteomes; UP000019116; Unplaced.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR InterPro; IPR000316; Metallthion_15.
DR Pfam; PF02068; Metallothio_PEC; 1.
DR PRINTS; PR00877; MTPLANTPEC.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Metal-binding;
KW Metal-thiolate cluster; Reference proteome; Zinc.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.2"
FT CHAIN 2..81
FT /note="EC protein I/II"
FT /id="PRO_0000197426"
FT CONFLICT 51
FT /note="R -> G (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 5..7
FT /evidence="ECO:0007829|PDB:2L61"
FT STRAND 14..16
FT /evidence="ECO:0007829|PDB:2L61"
FT HELIX 20..22
FT /evidence="ECO:0007829|PDB:2L61"
FT TURN 37..39
FT /evidence="ECO:0007829|PDB:2KAK"
FT STRAND 58..61
FT /evidence="ECO:0007829|PDB:2KAK"
FT HELIX 74..77
FT /evidence="ECO:0007829|PDB:2KAK"
SQ SEQUENCE 81 AA; 7713 MW; A08668901106B7A7 CRC64;
MGCDDKCGCA VPCPGGTGCR CTSARSGAAA GEHTTCGCGE HCGCNPCACG REGTPSGRAN
RRANCSCGAA CNCASCGSAT A
