ECC1B_MYCS2
ID ECC1B_MYCS2 Reviewed; 593 AA.
AC A0QNJ2;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=ESX-1 secretion system protein EccCb1 {ECO:0000305};
GN Name=eccCb1 {ECO:0000305};
GN Synonyms=Ms3871 {ECO:0000303|PubMed:15314236},
GN Sm3871 {ECO:0000303|PubMed:15687187}, snm2 {ECO:0000303|PubMed:15687187};
GN OrderedLocusNames=MSMEG_0062, MSMEI_0063;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=15314236; DOI=10.1073/pnas.0404892101;
RA Flint J.L., Kowalski J.C., Karnati P.K., Derbyshire K.M.;
RT "The RD1 virulence locus of Mycobacterium tuberculosis regulates DNA
RT transfer in Mycobacterium smegmatis.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:12598-12603(2004).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=15687187; DOI=10.1128/jb.187.4.1238-1245.2005;
RA Converse S.E., Cox J.S.;
RT "A protein secretion pathway critical for Mycobacterium tuberculosis
RT virulence is conserved and functional in Mycobacterium smegmatis.";
RL J. Bacteriol. 187:1238-1245(2005).
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=22233444; DOI=10.1111/j.1365-2958.2011.07958.x;
RA Wirth S.E., Krywy J.A., Aldridge B.B., Fortune S.M., Fernandez-Suarez M.,
RA Gray T.A., Derbyshire K.M.;
RT "Polar assembly and scaffolding proteins of the virulence-associated ESX-1
RT secretory apparatus in mycobacteria.";
RL Mol. Microbiol. 83:654-664(2012).
CC -!- FUNCTION: Part of the ESX-1 / type VII specialized secretion system
CC (T7SS), which exports several proteins including EsxA and EsxB
CC (PubMed:15687187). Plays a role in DNA conjugation, in both donor and
CC recipient strains (PubMed:15314236). {ECO:0000269|PubMed:15314236,
CC ECO:0000269|PubMed:15687187}.
CC -!- SUBUNIT: Part of the ESX-1 / type VII secretion system (T7SS), which is
CC composed of cytosolic and membrane components. The ESX-1 membrane
CC complex is composed of EccB1, EccCa1, EccCb1, EccD1 and EccE1 (By
CC similarity). {ECO:0000250|UniProtKB:P9WNB1}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Note=Localizes at or
CC near the cell pole in (on average) 1 discrete spot that also
CC colocalizes with SaeC. Localization requires SaeC and other ESX-1
CC proteins and can be conferred by the ESX-1 locus of M.tuberculosis
CC (PubMed:22233444). {ECO:0000269|PubMed:22233444}.
CC -!- DISRUPTION PHENOTYPE: Increases efficiency of DNA conjugation when
CC disrupted in donor strain (PubMed:15314236). Loss of secretion of EsxA
CC and EsxB, but not their expression (PubMed:15687187).
CC {ECO:0000269|PubMed:15314236, ECO:0000269|PubMed:15687187}.
CC -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC distinct donor and recipient strains; mc(2)155 is a donor strain while
CC MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC transfer do not always alter DNA transfer in a recipient strain.
CC {ECO:0000305|PubMed:15314236}.
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DR EMBL; CP000480; ABK71362.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP36545.1; -; Genomic_DNA.
DR RefSeq; WP_011726639.1; NZ_SIJM01000058.1.
DR RefSeq; YP_884480.1; NC_008596.1.
DR AlphaFoldDB; A0QNJ2; -.
DR SMR; A0QNJ2; -.
DR STRING; 246196.MSMEI_0063; -.
DR PRIDE; A0QNJ2; -.
DR EnsemblBacteria; ABK71362; ABK71362; MSMEG_0062.
DR EnsemblBacteria; AFP36545; AFP36545; MSMEI_0063.
DR GeneID; 66738252; -.
DR KEGG; msg:MSMEI_0063; -.
DR KEGG; msm:MSMEG_0062; -.
DR PATRIC; fig|246196.19.peg.60; -.
DR eggNOG; COG1674; Bacteria.
DR OMA; HRQEVWG; -.
DR OrthoDB; 7548at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR023837; EccCb-like_Actinobacteria.
DR InterPro; IPR002543; FtsK_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01580; FtsK_SpoIIIE; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR03925; T7SS_EccC_b; 1.
DR PROSITE; PS50901; FTSK; 2.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Nucleotide-binding; Reference proteome; Repeat;
KW Transport.
FT CHAIN 1..593
FT /note="ESX-1 secretion system protein EccCb1"
FT /id="PRO_0000438314"
FT DOMAIN 66..260
FT /note="FtsK 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT DOMAIN 350..546
FT /note="FtsK 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT BINDING 85..92
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT BINDING 377..384
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
SQ SEQUENCE 593 AA; 65057 MW; D1ECF00ABBCBEC03 CRC64;
MSTEAEPRVL REVVLSQLAT GESRAYKMWL PPLTDPTPVN ELVERDYQRR PLRFGLGIMD
EPRRHRQEVW GVDVSAAGGN IAVGGAPQTG KSTFLQTLVV SAAATHTPRQ VQFYCVDLGG
GGLMYLEDLP HVGGVATRAE PDRVNRVVAE VKAVLRAREQ VFKQYRVGSI ASYREMRDDP
NNPASQDPFG DVFLVIDGWP AFVAEFPDLE PAVQDIAGQG LAYGVHVIIT TPRWTELKSR
VRDYLGTKIE FRLGDVNETQ IDRITREIPA NRPGRAVSLE KHHLMMGVPR LDGVHSADNI
VEAISSAVQQ IADRHTDQAP QVRVLPERIY LHQLDPNPPG PDSDYRTRWQ VPLGVRESDL
TVAYNQMHLT PHLLIFGAPK SGKTRIAHAV AQAICKRNSP QQVRFMLADY RSGLLDAVPQ
SHLLDAGAIN RNSATLEEAI KALAVNLKKR LPPPDLTTAQ LRARSWWSGP DVVLLVDDWH
MVTAAAGMVS PMAPLGPLLP AAADIGLHVI VTCQMSMAHR ATMDKFVGAA YGAGSPTLFL
SGEKNDFPSR DIIVKKRPPG QAFLVGPDGK EVIQAAYVDP PEEEVFSPPS EGS