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ECC1B_MYCS2
ID   ECC1B_MYCS2             Reviewed;         593 AA.
AC   A0QNJ2;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=ESX-1 secretion system protein EccCb1 {ECO:0000305};
GN   Name=eccCb1 {ECO:0000305};
GN   Synonyms=Ms3871 {ECO:0000303|PubMed:15314236},
GN   Sm3871 {ECO:0000303|PubMed:15687187}, snm2 {ECO:0000303|PubMed:15687187};
GN   OrderedLocusNames=MSMEG_0062, MSMEI_0063;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=15314236; DOI=10.1073/pnas.0404892101;
RA   Flint J.L., Kowalski J.C., Karnati P.K., Derbyshire K.M.;
RT   "The RD1 virulence locus of Mycobacterium tuberculosis regulates DNA
RT   transfer in Mycobacterium smegmatis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:12598-12603(2004).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=15687187; DOI=10.1128/jb.187.4.1238-1245.2005;
RA   Converse S.E., Cox J.S.;
RT   "A protein secretion pathway critical for Mycobacterium tuberculosis
RT   virulence is conserved and functional in Mycobacterium smegmatis.";
RL   J. Bacteriol. 187:1238-1245(2005).
RN   [6]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=22233444; DOI=10.1111/j.1365-2958.2011.07958.x;
RA   Wirth S.E., Krywy J.A., Aldridge B.B., Fortune S.M., Fernandez-Suarez M.,
RA   Gray T.A., Derbyshire K.M.;
RT   "Polar assembly and scaffolding proteins of the virulence-associated ESX-1
RT   secretory apparatus in mycobacteria.";
RL   Mol. Microbiol. 83:654-664(2012).
CC   -!- FUNCTION: Part of the ESX-1 / type VII specialized secretion system
CC       (T7SS), which exports several proteins including EsxA and EsxB
CC       (PubMed:15687187). Plays a role in DNA conjugation, in both donor and
CC       recipient strains (PubMed:15314236). {ECO:0000269|PubMed:15314236,
CC       ECO:0000269|PubMed:15687187}.
CC   -!- SUBUNIT: Part of the ESX-1 / type VII secretion system (T7SS), which is
CC       composed of cytosolic and membrane components. The ESX-1 membrane
CC       complex is composed of EccB1, EccCa1, EccCb1, EccD1 and EccE1 (By
CC       similarity). {ECO:0000250|UniProtKB:P9WNB1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Note=Localizes at or
CC       near the cell pole in (on average) 1 discrete spot that also
CC       colocalizes with SaeC. Localization requires SaeC and other ESX-1
CC       proteins and can be conferred by the ESX-1 locus of M.tuberculosis
CC       (PubMed:22233444). {ECO:0000269|PubMed:22233444}.
CC   -!- DISRUPTION PHENOTYPE: Increases efficiency of DNA conjugation when
CC       disrupted in donor strain (PubMed:15314236). Loss of secretion of EsxA
CC       and EsxB, but not their expression (PubMed:15687187).
CC       {ECO:0000269|PubMed:15314236, ECO:0000269|PubMed:15687187}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:15314236}.
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DR   EMBL; CP000480; ABK71362.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP36545.1; -; Genomic_DNA.
DR   RefSeq; WP_011726639.1; NZ_SIJM01000058.1.
DR   RefSeq; YP_884480.1; NC_008596.1.
DR   AlphaFoldDB; A0QNJ2; -.
DR   SMR; A0QNJ2; -.
DR   STRING; 246196.MSMEI_0063; -.
DR   PRIDE; A0QNJ2; -.
DR   EnsemblBacteria; ABK71362; ABK71362; MSMEG_0062.
DR   EnsemblBacteria; AFP36545; AFP36545; MSMEI_0063.
DR   GeneID; 66738252; -.
DR   KEGG; msg:MSMEI_0063; -.
DR   KEGG; msm:MSMEG_0062; -.
DR   PATRIC; fig|246196.19.peg.60; -.
DR   eggNOG; COG1674; Bacteria.
DR   OMA; HRQEVWG; -.
DR   OrthoDB; 7548at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR023837; EccCb-like_Actinobacteria.
DR   InterPro; IPR002543; FtsK_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01580; FtsK_SpoIIIE; 2.
DR   SMART; SM00382; AAA; 2.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR03925; T7SS_EccC_b; 1.
DR   PROSITE; PS50901; FTSK; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Nucleotide-binding; Reference proteome; Repeat;
KW   Transport.
FT   CHAIN           1..593
FT                   /note="ESX-1 secretion system protein EccCb1"
FT                   /id="PRO_0000438314"
FT   DOMAIN          66..260
FT                   /note="FtsK 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT   DOMAIN          350..546
FT                   /note="FtsK 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT   BINDING         85..92
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT   BINDING         377..384
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
SQ   SEQUENCE   593 AA;  65057 MW;  D1ECF00ABBCBEC03 CRC64;
     MSTEAEPRVL REVVLSQLAT GESRAYKMWL PPLTDPTPVN ELVERDYQRR PLRFGLGIMD
     EPRRHRQEVW GVDVSAAGGN IAVGGAPQTG KSTFLQTLVV SAAATHTPRQ VQFYCVDLGG
     GGLMYLEDLP HVGGVATRAE PDRVNRVVAE VKAVLRAREQ VFKQYRVGSI ASYREMRDDP
     NNPASQDPFG DVFLVIDGWP AFVAEFPDLE PAVQDIAGQG LAYGVHVIIT TPRWTELKSR
     VRDYLGTKIE FRLGDVNETQ IDRITREIPA NRPGRAVSLE KHHLMMGVPR LDGVHSADNI
     VEAISSAVQQ IADRHTDQAP QVRVLPERIY LHQLDPNPPG PDSDYRTRWQ VPLGVRESDL
     TVAYNQMHLT PHLLIFGAPK SGKTRIAHAV AQAICKRNSP QQVRFMLADY RSGLLDAVPQ
     SHLLDAGAIN RNSATLEEAI KALAVNLKKR LPPPDLTTAQ LRARSWWSGP DVVLLVDDWH
     MVTAAAGMVS PMAPLGPLLP AAADIGLHVI VTCQMSMAHR ATMDKFVGAA YGAGSPTLFL
     SGEKNDFPSR DIIVKKRPPG QAFLVGPDGK EVIQAAYVDP PEEEVFSPPS EGS
 
 
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