ID   ECC1B_MYCSE             Reviewed;         593 AA.
AC   L8FPJ2; A0A2U9PH60;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=ESX-1 secretion system protein EccCb1;
GN   Name=eccCb1;
GN   ORFNames=0062 {ECO:0000303|PubMed:18554329},
GN   D806_000590 {ECO:0000312|EMBL:AWT51053.1}, D806_0062;
OS   Mycolicibacterium smegmatis (strain MKD8) (Mycobacterium smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=1214915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RX   PubMed=23618714; DOI=10.1128/genomea.00148-13;
RA   Gray T.A., Palumbo M.J., Derbyshire K.M.;
RT   "Draft genome sequence of MKD8, a conjugal recipient Mycobacterium
RT   smegmatis strain.";
RL   Genome Announc. 1:E0014813-E0014813(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RA   Derbyshire K., Gray T.A., Champion M.;
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MKD8;
RX   PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA   Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT   "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT   Mycobacterium smegmatis.";
RL   Mol. Microbiol. 69:794-808(2008).
CC   -!- FUNCTION: Part of the ESX-1 / type VII specialized secretion system
CC       (T7SS), which exports several proteins including EsxA and EsxB
CC       (PubMed:18554329). Plays a role in DNA conjugation, in both donor and
CC       recipient strains (PubMed:18554329). {ECO:0000269|PubMed:18554329}.
CC   -!- SUBUNIT: Part of the ESX-1 / type VII secretion system (T7SS), which is
CC       composed of cytosolic and membrane components. The ESX-1 membrane
CC       complex is composed of EccB1, EccCa1, EccCb1, EccD1 and EccE1 (By
CC       similarity). {ECO:0000250|UniProtKB:P9WNB1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Loss of DNA conjugation when disrupted in
CC       recipient strain, strain does not secrete EsxB (PubMed:18554329).
CC       {ECO:0000269|PubMed:18554329}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:18554329}.
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DR   EMBL; CP027541; AWT51053.1; -; Genomic_DNA.
DR   RefSeq; WP_003891389.1; NZ_CP027541.1.
DR   AlphaFoldDB; L8FPJ2; -.
DR   SMR; L8FPJ2; -.
DR   DIP; DIP-61400N; -.
DR   IntAct; L8FPJ2; 1.
DR   EnsemblBacteria; AWT51053; AWT51053; D806_000590.
DR   PATRIC; fig|1214915.3.peg.62; -.
DR   HOGENOM; CLU_003134_0_0_11; -.
DR   Proteomes; UP000011200; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR023837; EccCb-like_Actinobacteria.
DR   InterPro; IPR002543; FtsK_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01580; FtsK_SpoIIIE; 2.
DR   SMART; SM00382; AAA; 2.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR03925; T7SS_EccC_b; 1.
DR   PROSITE; PS50901; FTSK; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Nucleotide-binding; Repeat; Transport.
FT   CHAIN           1..593
FT                   /note="ESX-1 secretion system protein EccCb1"
FT                   /id="PRO_0000438315"
FT   DOMAIN          66..260
FT                   /note="FtsK 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT   DOMAIN          350..546
FT                   /note="FtsK 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT   BINDING         85..92
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT   BINDING         377..384
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
SQ   SEQUENCE   593 AA;  64972 MW;  4E0ED6B07AF5FCDC CRC64;
     MSTEAEPRVL REVVLSQLAT GESRAYKMWL PPLTDPTPVN ELVERDYQRR PLRFGLGIMD
     EPRRHRQEVW GVDVSAAGGN IAVGGAPQTG KSTFLQTLVV SAAATHTPRQ VQFYCVDLGG
     GGLMYLEDLP HVGGVATRAE PDRVNRVVAE VKAVLRAREQ VFKQYRVGSI ASYREMRDDP
     SNPASQDPFG DVFLVIDGWP AFVAEFPDLE PAVQDIAGQG LAYGVHVIIT TPRWTELKSR
     VRDYLGTKIE FRLGDVNETQ IDRITREIPA NRPGRAVSLE KHHLMMGVPR LDGVHSADNI
     VEAISSAVQQ IAGRHTDQAP QVRVLPERIY LHQLDPNPPG PDSDYRTRWQ VPLGVRESDL
     TVAYNQMHLT PHLLIFGAPK SGKTRIAHAV AQAICKRNSP QQVRFMLADY RSGLLDAVPQ
     SHLLDAGAIN RNSATLEEAI KALAVNLKKR LPPPDLTTAQ LRARSWWSGP DVVLLVDDWH
     MVTAAAGMVS PMAPLGPLLP AAADIGLHVI VTCQMSMAHR ATMDKFVGAA YGAGSPTLFL
     SGEKNDFPSR DIIVKKRPPG QAFLVGPDGK EVIQAAYVDP PEEEVFSPPS EGS