ECCA1_MYCS2
ID ECCA1_MYCS2 Reviewed; 574 AA.
AC A0QNI9; I7FVG3;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=ESX-1 secretion system protein EccA1 {ECO:0000303|PubMed:19876390};
DE AltName: Full=ESX conserved component A1 {ECO:0000303|PubMed:19876390};
DE AltName: Full=Type VII secretion system protein EccA1 {ECO:0000305};
DE Short=T7SS protein EccA1 {ECO:0000305};
GN Name=eccA1 {ECO:0000303|PubMed:19876390};
GN Synonyms=Ms3868 {ECO:0000303|PubMed:15314236},
GN Sm3868 {ECO:0000303|PubMed:15687187};
GN OrderedLocusNames=MSMEG_0059, MSMEI_0060;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=15314236; DOI=10.1073/pnas.0404892101;
RA Flint J.L., Kowalski J.C., Karnati P.K., Derbyshire K.M.;
RT "The RD1 virulence locus of Mycobacterium tuberculosis regulates DNA
RT transfer in Mycobacterium smegmatis.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:12598-12603(2004).
RN [5]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=15687187; DOI=10.1128/jb.187.4.1238-1245.2005;
RA Converse S.E., Cox J.S.;
RT "A protein secretion pathway critical for Mycobacterium tuberculosis
RT virulence is conserved and functional in Mycobacterium smegmatis.";
RL J. Bacteriol. 187:1238-1245(2005).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=MKD8;
RX PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT Mycobacterium smegmatis.";
RL Mol. Microbiol. 69:794-808(2008).
RN [7]
RP NOMENCLATURE.
RX PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT "Systematic genetic nomenclature for type VII secretion systems.";
RL PLoS Pathog. 5:E1000507-E1000507(2009).
CC -!- FUNCTION: Part of the ESX-1 / type VII specialized secretion system
CC (T7SS), which exports several proteins including EsxA and EsxB
CC (PubMed:15687187). Plays a role in DNA conjugation, in both donor and
CC recipient strains (PubMed:15314236, PubMed:18554329). EccA1 exhibits
CC ATPase activity and may provide energy for the export of ESX-1
CC substrates (By similarity). {ECO:0000250|UniProtKB:P9WPH9,
CC ECO:0000269|PubMed:15314236, ECO:0000269|PubMed:18554329}.
CC -!- SUBUNIT: Part of the ESX-1 / type VII secretion system (T7SS), which is
CC composed of cytosolic and membrane components (By similarity).
CC {ECO:0000250|UniProtKB:P9WPH9, ECO:0000305|PubMed:19876390}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B2HSU9}.
CC -!- DISRUPTION PHENOTYPE: Increases efficiency of DNA conjugation when
CC disrupted in donor strain (PubMed:15314236). Loss of DNA conjugation
CC when disrupted in recipient strain (PubMed:18554329) No effect on
CC secretion of EsxA or EsxB (PubMed:15687187). EsxB secretion was not
CC seen in this disruption in strain MDK8 (PubMed:18554329).
CC {ECO:0000269|PubMed:15314236, ECO:0000269|PubMed:15687187,
CC ECO:0000269|PubMed:18554329}.
CC -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC distinct donor and recipient strains; mc(2)155 is a donor strain while
CC MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC transfer do not always alter DNA transfer in a recipient strain.
CC {ECO:0000305|PubMed:15314236}.
CC -!- SIMILARITY: Belongs to the CbxX/CfxQ family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AFP36542.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CP000480; ABK74506.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP36542.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_003891386.1; NZ_SIJM01000058.1.
DR RefSeq; YP_884477.1; NC_008596.1.
DR AlphaFoldDB; A0QNI9; -.
DR SMR; A0QNI9; -.
DR STRING; 246196.MSMEI_0060; -.
DR TCDB; 3.A.24.5.1; the type vii or esx protein secretion system (t7ss) family.
DR PRIDE; A0QNI9; -.
DR EnsemblBacteria; ABK74506; ABK74506; MSMEG_0059.
DR EnsemblBacteria; AFP36542; AFP36542; MSMEI_0060.
DR GeneID; 66738249; -.
DR KEGG; msg:MSMEI_0060; -.
DR KEGG; msm:MSMEG_0059; -.
DR PATRIC; fig|246196.19.peg.57; -.
DR eggNOG; COG0464; Bacteria.
DR OMA; VEAGEQN; -.
DR OrthoDB; 1115436at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR Gene3D; 1.25.40.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041627; AAA_lid_6.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR000641; CbxX/CfxQ.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR023835; T7SS_EccA.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF17866; AAA_lid_6; 1.
DR PRINTS; PR00819; CBXCFQXSUPER.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR03922; T7SS_EccA; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Nucleotide-binding; Reference proteome; Transport.
FT CHAIN 1..574
FT /note="ESX-1 secretion system protein EccA1"
FT /id="PRO_0000438310"
FT BINDING 335..342
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000305"
SQ SEQUENCE 574 AA; 62148 MW; 20482F23E84D753D CRC64;
MVNHLAGMFG SAVGMMSNSR ARALEIFTEI TNYDESACDA WVGRIGCGDT DRVTLFRAWY
SRSNFGQLAG AAEISMNGVG ARIPIGGIYS RDITYPINSP LAITMGFAVH EASEGNYTDA
MEALEDAPAA GSEHLVSWVR AVIYGAGQRW TEVIDQVRGA ERWPDKFLAA AAGVAHGVAA
ANLGLFTEAD RRLTEANDTP VAQACAPVIA WYLAMARRSQ GNEESAQVLL EWLQANFPEP
KVTAALRDPA YRLETTTAEK IAARSDPWDP SSVVADTSAR DKLLVEAQAE LDRQIGLGRV
KEQIEAYRAA TQMARIRAAR GMKVAQTSKH MIFAGPPGTG KTTIARVVAN ILAGLGVIAE
PKLIETSRKD FVAEYEGQSA VKTSKTIDRA LGGVLFIDEA YTLVQERNGQ TDPFGAEALD
TLLARMENDR DRLVVIIAGY SNDIDRLLEV NDGLRSRFAT RIEFDSYSPD EIVEISKVIA
TANDSRLDDT AAKRVLEAAT TLSQRSLNGK PALDIAGNGR YARQLVEAGE QNRDMRLARS
LDFDSLGVDQ LSEINGDDMA AAIASVHSRL NIGE
